公开(公告)号：US10852521B2

公开(公告)日：2020-12-01

申请号：US16224397

申请日：2018-12-18

Applicant: LEICA BIOSYSTEMS IMAGING, INC.

Inventor： Yunlu Zou ,  Gregory Crandall ,  Allen Olson

IPC: G02B21/00 ,  G02B21/24 ,  G02B21/36

Abstract: Systems and methods for capturing a digital image of a slide using an imaging line sensor and a focusing line sensor. In an embodiment, a beam-splitter is optically coupled to an objective lens and configured to receive one or more images of a portion of a sample through the objective lens. The beam-splitter simultaneously provides a first portion of the one or more images to the focusing sensor and a second portion of the one or more images to the imaging sensor. A processor controls the stage and/or objective lens such that each portion of the one or more images is received by the focusing sensor prior to it being received by the imaging sensor. In this manner, a focus of the objective lens can be controlled using data received from the focusing sensor prior to capturing an image of a portion of the sample using the imaging sensor.

公开(公告)号：US10191264B2

公开(公告)日：2019-01-29

申请号：US15838043

申请日：2017-12-11

Applicant: Leica Biosystems Imaging, Inc.

Inventor： Yunlu Zou ,  Gregory Crandall ,  Allen Olson

IPC: G02B21/00 ,  G02B21/24 ,  G02B21/36

Abstract: Systems and methods for capturing a digital image of a slide using an imaging line sensor and a focusing line sensor. In an embodiment, a beam-splitter is optically coupled to an objective lens and configured to receive one or more images of a portion of a sample through the objective lens. The beam-splitter simultaneously provides a first portion of the one or more images to the focusing sensor and a second portion of the one or more images to the imaging sensor. A processor controls the stage and/or objective lens such that each portion of the one or more images is received by the focusing sensor prior to it being received by the imaging sensor. In this manner, a focus of the objective lens can be controlled using data received from the focusing sensor prior to capturing an image of a portion of the sample using the imaging sensor.

公开(公告)号：US09841590B2

公开(公告)日：2017-12-12

申请号：US14398443

申请日：2013-03-13

Applicant: LEICA BIOSYSTEMS IMAGING, INC.

Inventor： Yunlu Zou ,  Greg J. Crandall ,  Allen Olson

IPC: G02B21/00 ,  G02B21/36 ,  G02B21/04 ,  G02B21/24

CPC classification number: G02B21/006 ,  G02B21/245 ,  G02B21/361 ,  G02B21/365

Abstract: Systems and methods for capturing a digital image of a slide using an imaging line sensor and a focusing line sensor. In an embodiment, a beam-splitter is optically coupled to an objective lens and configured to receive one or more images of a portion of a sample through the objective lens. The beam-splitter simultaneously provides a first portion of the one or more images to the focusing sensor and a second portion of the one or more images to the imaging sensor. A processor controls the stage and/or objective lens such that each portion of the one or more images is received by the focusing sensor prior to it being received by the imaging sensor. In this manner, a focus of the objective lens can be controlled using data received from the focusing sensor prior to capturing an image of a portion of the sample using the imaging sensor.

公开(公告)号：US20150204784A1

公开(公告)日：2015-07-23

申请号：US14586337

申请日：2014-12-30

Applicant: Leica Biosystems Imaging, Inc.

Inventor： Gregory Crandall ,  Allen Olson

IPC: G01N21/64

CPC classification number: G01N21/64 ,  G01J3/0235 ,  G01J3/0264 ,  G01J3/027 ,  G01J3/4406 ,  G01N21/274 ,  G01N21/278 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/6458 ,  G01N2021/6471 ,  G02B21/365 ,  G06T5/009 ,  G06T5/40 ,  G06T7/74 ,  G06T7/80 ,  G06T2207/10024 ,  G06T2207/10056 ,  G06T2207/10064

Abstract: Systems and methods for standardizing one or more fluorescence scanning instruments to a reference system by separating the effects of drift and normalization. In an embodiment, a drift image comprising an image of a drift reference slide is captured by a system to be standardized. A drift measurement is calculated using the drift image. A first normalization image comprising an image of a normalization slide is also captured by the system to be standardized. A reference normalization image, also comprising an image of the normalization slide, is captured by a reference system. The first normalization image is compared to the reference normalization image to determine a gamma value and offset value for the system to be standardized.

Abstract translation: 通过分离漂移和归一化的影响将一个或多个荧光扫描仪器标准化到参考系统的系统和方法。 在一个实施例中，包括漂移参考滑块的图像的漂移图像由待标准化的系统捕获。 使用漂移图像计算漂移测量。 包括标准化幻灯片的图像的第一标准化图像也被要标准化的系统捕获。 还包括归一化幻灯片的图像的参考归一化图像由参考系统捕获。 将第一归一化图像与参考归一化图像进行比较，以确定要标准化的系统的伽马值和偏移值。