公开(公告)号：US4990450A

公开(公告)日：1991-02-05

申请号：US189135

申请日：1988-05-02

Applicant: Tatsurokuro Tochikura ,  Hidehiko Kumagai ,  Kenji Yamamoto ,  Setsu Kadowaki

Inventor： Tatsurokuro Tochikura ,  Hidehiko Kumagai ,  Kenji Yamamoto ,  Setsu Kadowaki

IPC: C12N9/24 ,  C12N9/38 ,  C12R1/05

CPC classification number: C12N9/2468 ,  C12Y302/01097 ,  Y10S435/829

Abstract: This invention provides endo-.alpha.-N-acetylgalactosaminidase (endo-.alpha.-GalNAcase) from a microorganism belonging to the genus Alcarigenes. This endo-.alpha.-GalNAcase is very useful in the analysis of the structure and function of mucin-type sugar chains of glycoproteins, as it is an enzyme that cleaves O-glycosidic linkages of sugar chains of glycoproteins, releasing the sugar chain from said protein.

公开(公告)号：US4894336A

公开(公告)日：1990-01-16

申请号：US237109

申请日：1988-07-13

Applicant: Karl Petzoldt ,  Helmut Dahl ,  Werner Skuballa

Inventor： Karl Petzoldt ,  Helmut Dahl ,  Werner Skuballa

IPC: C07C405/00 ,  C07D317/72 ,  C07D319/08 ,  C12P7/12 ,  C12P7/26 ,  C12P7/42 ,  C12P7/62 ,  C12P17/02 ,  C12P41/00 ,  C12R1/01 ,  C12R1/05 ,  C12R1/15 ,  C12R1/20 ,  C12R1/785 ,  C12R1/80 ,  C12R1/84 ,  C12R1/85 ,  C12R1/865 ,  C12R1/885

CPC classification number: C07D317/72 ,  C07C405/0083 ,  C07D319/08 ,  C12P17/02 ,  C12P41/004 ,  C12P7/26 ,  C12P7/42 ,  C12P7/62

Abstract: Process for the production of optically active (+)-bicyclo[3.3.0]octanol derivatives of formula (+)-I, in which R.sub.1 and R.sub.2 represent jointly an oxygen atom or the double-bond residue --O--X--O-- with X as a straight or branched-chain alkylene with 1-7 C-atoms, or R.sub.1 and R.sub.2 represent separately the residue OR.sub.5 with R.sub.5 as a straight or branched-chain alkyl with 1-7 C-atoms, and R.sub.3 the residue COOZ with Z as a hydrogen atom, straight or branched chain alkyl with 1-7 C atoms, cycloalkyl with 3-6 C atoms, phenyl or aralkyl with 7-10 atoms or R.sub.3 is the residue --(CH.sub.2).sub.n --O--COR.sub.4 with n having the meaning 1-4 and R.sub.4 as a straight or branched-chain alkyl with 1-7 C atoms, cycloalkyl with 3-6 C atoms, phenyl or aralkyl with 7-10 C atoms. The process is characterized in that racemic 3.alpha.-cyloxy-cis-bicyclo[3.3.0]-octane derivatives of formula (+)-II, wherein R.sub.1, R.sub.2 R.sub.3 and R.sub.4 have the above meanings, are subjected enzymatically or microbiologically to a stereospecific acylate hydrolsis and the (+)-bicyclo[3.3.0]-octanol derivative produced is separated from the unsaponified bicyclo[3.3.0]-octanol acylate of formula (-)-II or the unsaponified enantiomers (+)-II are separated from the saponified bicyclo[3.3.0]octanol derivative (-)-I and then subjected to chemical acylate hydrolysis.

Abstract translation: PCT No.PCT / DE87 / 00513 Sec。 371日期：1988年7月13日 102（e）日期1988年7月13日PCT提交1987年11月12日PCT公布。 出版物WO88 / 03567 日期：1988年5月19日。制备式（+） - I的光学活性（+） - 双环[3.3.0]辛醇衍生物的方法，其中R 1和R 2共同表示氧原子或双键残基-OXO - X为具有1-7个C原子的直链或支链亚烷基，或者R1和R2分别表示残基OR5与R5作为具有1-7个C原子的直链或支链烷基，R3表示残基 具有Z作为氢原子的COOZ，具有1-7个C原子的直链或支链烷基，具有3-6个C原子的环烷基，具有7-10个原子的苯基或芳烷基或R3是残基 - （CH2）nO-COR4，其中n 具有1-4和R4为具有1-7个C原子的直链或支链烷基，具有3-6个C原子的环烷基，具有7-10个C原子的苯基或芳烷基。 该方法的特征在于其中R1，R2R3和R4具有上述含义的式（+） - II的外消旋3α-甲氧基 - 顺式 - 双 - 二环[3.3.0] - 辛烷衍生物经酶促或微生物方式进行立体特异性 将酰化物水解和（+） - 双环[3.3.0] - 辛醇衍生物与式（ - ） - II的未皂化的双环[3.3.0] - 辛醇酰化物分离，或未分离的对映异构体（+） - 从皂化的双环[3.3.0]辛醇衍生物（ - ） - I中，然后进行化学酰化物水解。

公开(公告)号：US4891317A

公开(公告)日：1990-01-02

申请号：US719505

申请日：1985-04-03

Applicant: R. Malcolm Brown, Jr. ,  Debra S. Brown ,  Michael R. Gretz

Inventor： R. Malcolm Brown, Jr. ,  Debra S. Brown ,  Michael R. Gretz

IPC: C08B15/00 ,  A01G7/04 ,  C08B1/00 ,  C12N13/00 ,  C12P19/04 ,  C12R1/01 ,  C12R1/02 ,  C12R1/05 ,  C12R1/38 ,  C12R1/41

CPC classification number: C12N13/00 ,  A01G7/04 ,  C08B1/00 ,  C12P19/04 ,  Y10S435/822

Abstract: A method of producing cellulose of amorphous character by subjecting cellulose-producing organisms to a magnetic field substantially greater than 0.5 gauss and preferably at least about 500 gauss. The cellulose produced in the presence of a magnetic field is of an amorphous nature with increased water absorptivity and decreased crystallinity.

Abstract translation: 通过使产生纤维素的生物体达到基本上大于0.5高斯，优选至少约500高斯的磁场，制备无定形特性的纤维素的方法。 在磁场存在下产生的纤维素具有无定形性质，具有增加的吸水率和降低的结晶度。

公开(公告)号：US4739046A

公开(公告)日：1988-04-19

申请号：US767388

申请日：1985-08-19

Applicant: Nicholas R. Di Luzio

Inventor： Nicholas R. Di Luzio

IPC: C12P19/04 ,  A61K31/715 ,  A61P31/00 ,  A61P31/04 ,  A61P35/00 ,  A61P37/00 ,  C07H3/06 ,  C08B1/00 ,  C08B37/00 ,  C08B37/18 ,  C12R1/05 ,  C12R1/46 ,  C12R1/645 ,  C12R1/72 ,  C12R1/84 ,  C12R1/85 ,  C12R1/865 ,  A61K31/66

CPC classification number: C08B37/0024 ,  Y10S514/908

Abstract: A new class of soluble phosphorylated glucans is described as well as the process for making the same. According to a preferred embodiment, the soluble phosphorylated glucan is derived from the yeast Saccharomyces cerevisiae. The soluble phosphorylated glucans are useful for prophylactic and therapeutic applications against neoplastic, bacteria, viral, fungal and parasitic diseases. Additionally, they may be administered as a non-toxic adjuvant, in combination with chemotherapy. The soluble phosphorylated glucans are also useful for stimulating macrophage cells, either in vivo or in vitro, to produce a cytotoxic/cyctostatic factor effective against cancer cells.

Abstract translation: 描述了一类新的可溶性磷酸化葡聚糖以及其制备方法。 根据优选的实施方案，可溶性磷酸化葡聚糖衍生自酿酒酵母酿酒酵母（Saccharomyces cerevisiae）。 可溶性磷酸化葡聚糖可用于预防和治疗抗肿瘤，细菌，病毒，真菌和寄生虫病的应用。 另外，它们可以作为无毒佐剂与化学疗法组合施用。 可溶性磷酸化葡聚糖也可用于在体内或体外刺激巨噬细胞以产生对癌细胞有效的细胞毒性/细胞因子。

公开(公告)号：US4705604A

公开(公告)日：1987-11-10

申请号：US746381

申请日：1985-06-19

Applicant: Noel Vanlautem ,  Jacques Gilain

Inventor： Noel Vanlautem ,  Jacques Gilain

IPC: C12P7/62 ,  C12R1/05 ,  B01D3/36 ,  C07C69/66 ,  C08G63/06

CPC classification number: C12P7/625

Abstract: A process for extracting poly-beta-hydroxybutyrates from an aqueous suspension of microorganisms, in which an azeotropic distillation, and an extraction of the polymer by means of the solvent used, are carried out simultaneously.The poly-beta-hydroxybutyrates obtained can be used in medicine.

Abstract translation: 从微生物的水性悬浮液中提取聚-β-羟基丁酸的方法，其中共沸蒸馏和使用的溶剂萃取聚合物，同时进行。 获得的聚-β-羟基丁酸酯可用于医药。

公开(公告)号：US4622188A

公开(公告)日：1986-11-11

申请号：US684947

申请日：1984-12-21

Applicant: Marina Adamich ,  David T. Bach

Inventor： Marina Adamich ,  David T. Bach

IPC: A61K9/127 ,  C12R1/05 ,  B01J13/02

CPC classification number: A61K9/1277 ,  Y10S436/829

Abstract: A method and apparatus for manufacturing liposomes is characterized by optically monitoring the light transmission of a liposome precursor solution and controlling the flow rate and/or temperature of the precursor solution and/or a dialysis buffer solution in accordance therewith.

Abstract translation: 用于制造脂质体的方法和装置的特征在于通过光学监测脂质体前体溶液的光透射并根据其控制前体溶液和/或透析缓冲溶液的流速和/或温度。

公开(公告)号：US4600692A

公开(公告)日：1986-07-15

申请号：US518756

申请日：1983-07-29

Applicant: Louis L. Wood ,  Gary J. Calton

Inventor： Louis L. Wood ,  Gary J. Calton

IPC: C12N9/10 ,  C12N11/02 ,  C12N11/08 ,  C12P13/06 ,  C12P13/20 ,  C12P13/22 ,  C12P19/24 ,  C12P33/02 ,  C12P37/06 ,  C12R1/01 ,  C12R1/025 ,  C12R1/05 ,  C12R1/19 ,  C12R1/285 ,  C12R1/38 ,  C12R1/645 ,  C12R1/865 ,  C12N11/04

CPC classification number: C12N11/08 ,  C12N11/02 ,  C12N9/1096 ,  C12P13/06 ,  C12P13/20 ,  C12P13/222 ,  C12P19/24 ,  C12P33/02 ,  C12P37/06

Abstract: A process is disclosed for preparing phenylalanine which comprises contacting phenylpyruvic acid or phenylpyruvate with immobilized whole cells having transaminase activity in the presence of an amine donor. The cells are preferably immobilized with a polyazetidine polymer. Ruptured or permeabilized cells, with the enzyme in the free or immobilized state, may also be used. The preparation of phenylalanine from cinnamic acid using immobilized cells having phenylalanine ammonia lyase activity is also disclosed.

Abstract translation: 公开了一种制备苯丙氨酸的方法，该方法包括使苯丙酮酸或苯丙酮酸与在胺供体存在下具有转氨酶活性的固定的全细胞接触。 细胞优选用多氮杂环丁烷聚合物固定。 也可以使用具有游离或固定状态的酶的破裂或透化细胞。 还公开了使用具有苯丙氨酸氨裂解酶活性的固定化细胞从肉桂酸制备苯丙氨酸。

公开(公告)号：US4540661A

公开(公告)日：1985-09-10

申请号：US469375

申请日：1983-02-24

Applicant: Betty R. Hannon ,  Fritz Reusser ,  Lester A. Dolak ,  Alexander D. Argoudelis ,  Thomas M. Castle

Inventor： Betty R. Hannon ,  Fritz Reusser ,  Lester A. Dolak ,  Alexander D. Argoudelis ,  Thomas M. Castle

IPC: C12P13/00 ,  C12P39/00 ,  C12N1/20 ,  C12R1/05 ,  C12R1/465

CPC classification number: C12P13/008 ,  C12R1/025 ,  C12R1/465 ,  Y10S435/829 ,  Y10S435/886

Abstract: Antibiotic U-66,026 is produced in a fermentation under controlled conditions using the microorganism Alcaligenes sp., NRRL B-15269. Enhanced fermentation of titers U-66,026 are obtained when Alcaligenes sp., NRRL B-15269, is cultivated in mixture with Streptomyces plicatus strain 395, NRRL 15273.Antibiotic U-66,026 is a useful antibiotic which has antifungal activity.

Abstract translation: 在受控条件下使用微生物产碱杆菌属，NRRL B-15269在发酵中产生抗生素U-66,026。 当产碱杆菌属物种NRRL B-15269与Streptomyces plicatus菌株395，NRRL 15273的混合培养时获得增强的滴度U-66,026的发酵。抗生素U-66,026是具有抗真菌活性的有用的抗生素。

公开(公告)号：US4503147A

公开(公告)日：1985-03-05

申请号：US435316

申请日：1982-10-19

Applicant: Motoo Nakajima ,  Kazuo Nakamura ,  Yoshio Shirokane ,  Kiyoshi Mizusawa

Inventor： Motoo Nakajima ,  Kazuo Nakamura ,  Yoshio Shirokane ,  Kiyoshi Mizusawa

IPC: C12N9/06 ,  C12Q1/26 ,  C12R1/05 ,  C12R1/07

CPC classification number: C12N9/0022 ,  C12Q1/26 ,  Y10S435/829 ,  Y10S435/832

Abstract: A monomethylamine-oxidizing enzyme can be obtained by cultivating in a medium a strain which belongs to Genus Bacillus and has an ability to produce a monomethylamine-oxidizing enzyme. This enzyme exhibits several beneficial properties including the ability to oxidatively deaminate the amino group of monomethylamine to produce formaldehyde, ammonia, and hydrogen peroxide. The enzyme exhibits a high substrate specificity for monomethylamine, ethylamine, and n-proplyamine while showing no substrate specificity for benzylamine, dimethylamine, trimethylamine, ethylenediamine and tryamine. In addition, the enzyme is stable through an elevated temperature range permitting faster reaction rates and therefore a shorter overall quantitative evaluation. Another property includes a low Km value which allows smaller quantities of the enzyme to be employed per sample.

Abstract translation: 通过在培养基中培养属于芽孢杆菌属的菌株并具有产生单甲胺氧化酶的能力，可以获得单甲胺氧化酶。 该酶显示出几种有益的性质，包括使一甲胺的氨基氧化脱氨以产生甲醛，氨和过氧化氢的能力。 该酶对单甲胺，乙胺和正丙胺显示出高的底物特异性，同时对苄胺，二甲胺，三甲胺，乙二胺和试胺没有底物特异性。 此外，酶在升高的温度范围内是稳定的，允许更快的反应速率，因此较短的总体定量评估。 另一种性质包括低Km值，其允许每个样品使用较少量的酶。

公开(公告)号：US4481362A

公开(公告)日：1984-11-06

申请号：US494692

申请日：1983-05-17

Applicant: Mamoru Nakai ,  Tokio Ohshima ,  Tomio Kimura ,  Tetsuo Omata ,  Noritada Iwamoto

Inventor： Mamoru Nakai ,  Tokio Ohshima ,  Tomio Kimura ,  Tetsuo Omata ,  Noritada Iwamoto

IPC: C12N9/80 ,  C12P13/04 ,  C12P13/22 ,  C12P41/00 ,  C12R1/025 ,  C12R1/05 ,  C12R1/06 ,  C12R1/07 ,  C12R1/13 ,  C12R1/145 ,  C12R1/20 ,  C12R1/225 ,  C12R1/265 ,  C12R1/32 ,  C12R1/365 ,  C12R1/38 ,  C12R1/46 ,  C12R1/465 ,  C12R1/645 ,  C12R1/65 ,  C12R1/66 ,  C12R1/72 ,  C12R1/77 ,  C12R1/78 ,  C12R1/80 ,  C12R1/84 ,  C12R1/845 ,  C12R1/85 ,  C12R1/88 ,  C12R1/885 ,  C07D209/20

CPC classification number: C12N9/80 ,  C12P13/04 ,  C12P13/227

Abstract: Biochemical optical resolution of DL-tryptophanes in which DL-tryptophane amides are interacted with the culture products, or their treated products, of a microorganism capable of producing amidase is described. L-tryptophane amides in racemic DL-tryptophane amides are asymmetrically hydrolyzed to form optically active L-tryptophanes at a high yield and almost all D-tryptophane amides remain without being subjected to hydrolysis. The resultant D-tryptophane amides are readily hydrolyzed, after separating L-tryptophanes, to form optically active D-tryptophanes at a high yield.

Abstract translation: 描述DL-色氨酰胺与能够产生酰胺酶的微生物或其处理产物相互作用的DL-色氨酸的生物化学光学拆分。 外消旋的DL-色氨酰胺中的L-色氨酰胺不对称地水解，以高产率形成光学活性的L-色氨酸，并且几乎所有的D-色氨酸酰胺保持不被水解。 得到的D-色氨酰胺在分离L-色氨酸后容易水解，以高产率形成光学活性的D-色氨酸。