公开(公告)号：US20120018306A1

公开(公告)日：2012-01-26

申请号：US13249425

申请日：2011-09-30

Applicant: Vijay Srinivasan ,  Vamsee K. Pamula ,  Michael G. Pollack

Inventor： Vijay Srinivasan ,  Vamsee K. Pamula ,  Michael G. Pollack

IPC: C25B15/00 ,  C25B7/00

CPC classification number: B01L3/502784 ,  B01J19/0046 ,  B01J2219/00466 ,  B01J2219/00495 ,  B01J2219/00587 ,  B01J2219/00725 ,  B01L3/502761 ,  B01L2200/0642 ,  B01L2200/0647 ,  B01L2300/0819 ,  B01L2300/0829 ,  B01L2300/0864 ,  B01L2300/0887 ,  B01L2300/0893 ,  B01L2300/18 ,  B01L2400/0427 ,  B01L2400/043 ,  C07K1/1136 ,  C30B7/00 ,  C30B29/58 ,  Y10T436/2575

Abstract: Sample processing droplet actuators, systems and methods are provided. According to one embodiment, a stamping device including a droplet microactuator is provided and includes: (a) a first plate including a path or network of control electrodes for transporting droplets on a surface thereof; (b) a second plate mounted in a substantially parallel orientation with respect to the first plate providing an interior volume between the plates, the second plate including one or more stamping ports for transporting some portion or all of a droplet from the interior volume to an exterior location; (c) a port for introducing fluid into the interior volume between the plates; and (d) a path or network of reference electrodes corresponding to the path or network of control electrodes. Associated systems and methods including the stamping device are also provided.

Abstract translation: 提供样品处理液滴致动器，系统和方法。 根据一个实施例，提供了一种包括液滴微致动器的冲压装置，其包括：（a）包括用于在其表面上输送液滴的控制电极的路径或网络的第一板; （b）相对于第一板以基本上平行的方向安装的第二板，提供板之间的内部容积，第二板包括一个或多个冲压端口，用于将一些部分或全部液滴从内部容积输送到 外部位置; （c）用于将流体引入板之间的内部容积的端口; 和（d）对应于控制电极的路径或网络的参考电极的路径或网络。 还提供了包括冲压装置在内的相关系统和方法。

公开(公告)号：US08041463B2

公开(公告)日：2011-10-18

申请号：US12707081

申请日：2010-02-17

Applicant: Michael G. Pollack ,  Philip Paik

Inventor： Michael G. Pollack ,  Philip Paik

IPC: G05D7/00 ,  G05D11/00 ,  G01N7/00 ,  B05C11/00

CPC classification number: B01L3/502792 ,  B01L2200/025 ,  B01L2200/04 ,  B01L2300/089 ,  B01L2400/0427 ,  G01N21/59 ,  G01N2021/035 ,  G01N2035/1046

Abstract: A droplet actuator drive including a detection apparatus for sensing a property of a droplet on a droplet actuator; circuitry for controlling the detection apparatus electronically coupled to the detection apparatus; a droplet actuator cartridge connector arranged so that when a droplet actuator cartridge electronically is coupled thereto: the droplet actuator cartridge is aligned with the detection apparatus; and the detection apparatus can sense the property of the droplet on a droplet actuator; circuitry for controlling a droplet actuator coupled to the droplet actuator connector; and the droplet actuator circuitry may be coupled to a processor.

Abstract translation: 液滴致动器驱动器，其包括用于感测液滴致动器上的液滴的特性的检测装置; 用于控制检测装置的电路，电子耦合到所述检测装置; 液滴致动器盒连接器布置成使得当液滴致动器盒电子地耦合到其上时：液滴致动器盒与检测装置对准; 并且检测装置可以感测液滴致动器上的液滴的特性; 用于控制耦合到液滴致动器连接器的液滴致动器的电路; 并且液滴致动器电路可以耦合到处理器。

公开(公告)号：US20110114490A1

公开(公告)日：2011-05-19

申请号：US12985409

申请日：2011-01-06

Applicant: Vamsee K. Pamula ,  Arjun Sudarsan ,  Ramakrishna Sista

Inventor： Vamsee K. Pamula ,  Arjun Sudarsan ,  Ramakrishna Sista

IPC: B01J8/08

CPC classification number: B01F11/0071 ,  B01F13/0071 ,  B01F13/0076 ,  B01L3/502715 ,  B01L3/502761 ,  B01L3/502784 ,  B01L3/502792 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/027 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/089 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0436 ,  B01L2400/0442 ,  B03C1/01 ,  B03C1/288 ,  B03C2201/18 ,  G01N2035/1046

Abstract: The invention provides a method of redistributing magnetically responsive beads in a droplet. The method may include providing a droplet including magnetically responsive beads. The droplet may be provided within a region of a magnetic field having sufficient strength to attract the magnetically responsive beads to an edge of the droplet or towards an edge of the droplet, or otherwise regionalize or aggregate beads within the droplet. The method may also include conducting on a droplet operations surface one or more droplet operations using the droplet without removing the magnetically responsive beads from the region of the magnetic field. The droplet operations may in some cases be electrode-mediated. The droplet operations may redistribute and/or circulate the magnetically responsive beads within the droplet. In some cases, the droplet may include a sample droplet may include a target analyte. The redistributing of the magnetically responsive beads may cause target analyte to bind to the magnetically responsive beads. In some cases, the droplet may include unbound substances in a wash buffer. The redistributing of the magnetically responsive beads causes unbound substances to be freed from interstices of an aggregated set or subset of the magnetically responsive beads.

Abstract translation: 本发明提供了一种在液滴中重新分布磁响应珠的方法。 该方法可以包括提供包括磁响应珠的液滴。 液滴可以设置在具有足够强度的磁场的区域内，以将磁响应珠吸引到液滴的边缘或朝向液滴的边缘，或以其他方式区分或聚集液滴内的珠粒。 该方法还可以包括在液滴操作表面上使用液滴进行一个或多个液滴操作，而不从磁场的区域移除磁响应珠。 在某些情况下，液滴操作可能是电极介导的。 液滴操作可以在液滴内重新分布和/或循环磁响应珠。 在一些情况下，液滴可以包括样品液滴可以包括靶分析物。 磁响应珠的重新分布可能导致目标分析物结合磁响应珠。 在一些情况下，液滴可以包括洗涤缓冲液中的未结合物质。 磁响应珠子的重新分布导致未结合物质从磁性响应珠粒的聚集或子集的空隙中释放出来。

公开(公告)号：US20110100823A1

公开(公告)日：2011-05-05

申请号：US12960583

申请日：2010-12-06

Applicant: Michael G. Pollack ,  Philip Y. Paik ,  Vamsee K. Pamula

Inventor： Michael G. Pollack ,  Philip Y. Paik ,  Vamsee K. Pamula

IPC: G01N21/76 ,  G01N27/447

CPC classification number: C12Q1/68 ,  B01L7/52 ,  B01L2300/0645 ,  B01L2300/1822 ,  B01L2300/1894 ,  B01L2400/0424 ,  B01L2400/0427 ,  C12Q1/6869 ,  G01N27/44791 ,  Y10T436/25 ,  C12Q2565/629 ,  C12Q2565/301 ,  C12Q2563/149

Abstract: The present invention relates to a droplet-based nucleic acid amplification apparatus and system. According to one embodiment, a droplet microactuator is provided made using a first substrate including a fluorescing material and including a detection region for detecting a fluorescence signal from a droplet, which detection region is coated with a light absorbing, low fluorescence or non-fluorescing material.

Abstract translation: 本发明涉及一种基于液滴的核酸扩增装置和系统。 根据一个实施例，使用包括荧光材料的第一基底并且包括用于检测来自液滴的荧光信号的检测区域来提供液滴微致动器，该检测区域被光吸收，低荧光或非荧光材料涂覆 。

公开(公告)号：US20110097763A1

公开(公告)日：2011-04-28

申请号：US12991966

申请日：2009-05-13

Applicant: Michael G. Pollack ,  Zhishan Hua ,  Allen E. Eckhardt ,  Prasanna Thwar ,  Vijay Srinivasan ,  Vamsee K. Pamula

Inventor： Michael G. Pollack ,  Zhishan Hua ,  Allen E. Eckhardt ,  Prasanna Thwar ,  Vijay Srinivasan ,  Vamsee K. Pamula

IPC: C12P19/34

CPC classification number: B01L7/525 ,  B01L3/502761 ,  B01L3/502792 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/0668 ,  B01L2200/10 ,  B01L2300/0803 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/088 ,  B01L2300/0883 ,  B01L2300/089 ,  B01L2300/1827 ,  B01L2400/0427 ,  B01L2400/043 ,  C12Q1/686 ,  C12Q2565/629

Abstract: The invention relates to certain novel approaches to reducing or eliminating the movement of contaminants from one droplet to another on a droplet actuator via liquid filler fluid. In one application, droplet actuators are used to conduct genetic analysis using polymerase chain reaction (PCR) techniques. The invention addresses the need for improved methods of performing PCR on a droplet actuator that provide for optimum amplification and detection of a sample target.

Abstract translation: 本发明涉及在液滴致动器上通过液体填充液减少或消除污染物从一个液滴到另一个液滴的移动的某些新颖方法。 在一个应用中，液滴致动器用于使用聚合酶链反应（PCR）技术进行遗传分析。 本发明解决了对提供样品靶的最佳放大和检测的在液滴致动器上进行PCR的改进方法的需要。

公开(公告)号：US20100270156A1

公开(公告)日：2010-10-28

申请号：US12682830

申请日：2008-12-23

Applicant: Vijay Srinivasan ,  Michael G. Pollack ,  Vamsee K. Pamula ,  Zhishan Hua ,  Arjun Sudarsan ,  Philip Paik

Inventor： Vijay Srinivasan ,  Michael G. Pollack ,  Vamsee K. Pamula ,  Zhishan Hua ,  Arjun Sudarsan ,  Philip Paik

IPC: C07K1/26

CPC classification number: B01L3/52 ,  B01F13/0071 ,  B01F13/0076 ,  B01L3/0241 ,  B01L3/502792 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2200/143 ,  B01L2200/16 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/089 ,  B01L2400/0427 ,  B01L2400/06 ,  B01L2400/0688 ,  F04B19/006

Abstract: A droplet actuator with a droplet formation electrode configuration associated with a droplet operations surface, wherein the electrode configuration comprises one or more electrodes configured to control volume of a droplet during formation of a sub-droplet on the droplet operations surface. Methods of making and using the droplet actuator are also provided.

Abstract translation: 具有与液滴操作表面相关联的液滴形成电极配置的液滴致动器，其中所述电极配置包括一个或多个电极，其被配置为在液滴操作表面上形成子液滴期间控制液滴的体积。 还提供了制造和使用液滴致动器的方法。

公开(公告)号：US07763471B2

公开(公告)日：2010-07-27

申请号：US11839762

申请日：2007-08-16

Applicant: Vamsee K. Pamula ,  Vijay Srinivasan ,  Michael G. Pollack

Inventor： Vamsee K. Pamula ,  Vijay Srinivasan ,  Michael G. Pollack

IPC: G01N33/00

CPC classification number: G01N23/20 ,  B01L3/06 ,  B01L3/502792 ,  B01L2200/16 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0421 ,  B01L2400/0427 ,  Y10T436/25

Abstract: Methods of using protein crystallization droplet actuators are provided. Protein sample droplets and reagent droplets are dispensed, transported, and merged to yield an array of crystallization conditions by electrowetting droplet operations in a gap comprising oil filler fluid. The oil filler fluid is doped with a surfactant that enhances droplet operations using the protein sample.

Abstract translation: 提供了使用蛋白质结晶液滴致动器的方法。 蛋白质样品液滴和试剂液滴被分配，运输和合并，以通过在包含填充油的间隙中的电润湿液滴操作产生一系列结晶条件。 油填充液掺杂有使用蛋白质样品增强液滴操作的表面活性剂。

公开(公告)号：US20100032293A1

公开(公告)日：2010-02-11

申请号：US12531809

申请日：2008-04-10

Applicant: Michael G. Pollack ,  Vamsee K. Pamula ,  Vijay Srinivasan

Inventor： Michael G. Pollack ,  Vamsee K. Pamula ,  Vijay Srinivasan

IPC: C07K1/26 ,  G01N27/00

CPC classification number: B01L3/502761 ,  B01F13/0071 ,  B01F13/0076 ,  B01L3/502792 ,  B01L2200/0605 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2200/16 ,  B01L2300/0819 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/088 ,  B01L2300/089 ,  B01L2400/0427 ,  B01L2400/043 ,  G01N27/447

Abstract: The invention provides nonlimiting examples of structures for and methods of dispensing droplets in a droplet actuator. The droplet actuator structures and methods of the invention exhibit numerous advantages over droplet actuators of the prior art. In various embodiments, the structures and methods of the invention provide, among other things, improved efficiency, throughput, scalability, and/or droplet uniformity, as compared with existing droplet actuators. Further, in some embodiments, the droplet actuators provide configurations for improved methods of loading and/or unloading fluid and/or droplets. In yet other embodiments, the droplet actuators provide fluid loading configurations for loading numerous fluid reservoirs in a substantially simultaneous and/or substantially sequential manner.

Abstract translation: 本发明提供了用于在液滴致动器中分配液滴的结构的方法和方法的非限制性实例。 本发明的液滴致动器结构和方法与现有技术的液滴致动器相比表现出许多优点。 在各种实施例中，与现有的液滴致动器相比，本发明的结构和方法尤其提供了改进的效率，吞吐量，可扩展性和/或液滴均匀性。 此外，在一些实施例中，液滴致动器提供用于加载和/或卸载流体和/或液滴的改进方法的构造。 在其他实施例中，液滴致动器提供用于以基本上同时和/或基本顺序的方式装载多个流体储存器的流体加载构造。

公开(公告)号：USD599832S1

公开(公告)日：2009-09-08

申请号：US29304063

申请日：2008-02-25

Applicant: David Schoch Chapin ,  Daniel M. Stipe

Designer： David Schoch Chapin ,  Daniel M. Stipe

公开(公告)号：US20230128722A1

公开(公告)日：2023-04-27

申请号：US18063874

申请日：2022-12-09

Applicant: Advanced Liquid Logic, Inc.

Inventor： Vamsee K. Pamula ,  Michael G. Pollack ,  Ramakrishna Sista ,  Arjun Sudarsan

IPC: G01N33/543 ,  B01L3/00 ,  B03C1/02 ,  B03C1/28 ,  B03C1/30 ,  B03C5/00 ,  C12N15/10 ,  G01N33/561 ,  B01F31/65 ,  B01F33/302 ,  B01F33/3031

Abstract: Methods are provided for separating magnetically responsive beads from a droplet in a droplet actuator. Droplet operations electrodes and a magnet are arranged in a droplet actuator to manipulate a bead-containing droplet and position it relative to a magnetic field region that attracts the magnetically responsive beads. The droplet operations electrodes are operated to control the droplet shape and transport it away from the magnetic field region to form a concentration of beads in the droplet. The continued transport of the droplet away from the magnetic field causes the concentration of beads to break away from the droplet to yield a small, concentrated bead-containing droplet immobilized by the magnet.