公开(公告)号：US11996169B2

公开(公告)日：2024-05-28

申请号：US17808880

申请日：2022-06-24

Applicant: Life Technologies Corporation

Inventor： Nivedita Sumi Majumdar

IPC: G16B45/00 ,  G06F16/16 ,  G06F16/26 ,  G06T11/20 ,  G16B20/00 ,  G16B40/00

CPC classification number: G16B45/00 ,  G06F16/168 ,  G06F16/26 ,  G06T11/206 ,  G16B20/00 ,  G16B40/00

Abstract: In one exemplary embodiment, a method computer-implemented method of generating an outlier wheel data visualization for a graphical user interface (GUI) is provided. The method includes receiving emission data from a plurality of amplification reactions. The method further includes generating an outlier wheel data visualization using the emission data including a plurality of lines, where each line included in the outlier wheel data visualization represents emission data from an amplification reaction of the plurality of amplification reactions, and each line having a length and a visual indicator. The length of each line represents growth of intensity of fluorescence of the emission data and the visual indicator indicates the associated cycle number of the emission data. Further, each line is associated with an angular position so that the plurality of lines is configured in a circular shape. The method includes displaying the outlier wheel data visualization on a GUI.

公开(公告)号：US20240159702A1

公开(公告)日：2024-05-16

申请号：US18353017

申请日：2023-07-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan M. ROTHBERG ,  Wolfgang HINZ ,  Kim L. JOHNSON ,  James BUSTILLO

IPC: G01N27/414 ,  C12Q1/6818 ,  C12Q1/6874 ,  G01N33/543 ,  H01L27/088 ,  H01L29/78

CPC classification number: G01N27/4148 ,  C12Q1/6818 ,  C12Q1/6874 ,  G01N27/4145 ,  G01N33/54373 ,  G01N33/5438 ,  H01L27/088 ,  H01L29/78 ,  H01L29/42324 ,  H01L2924/01006 ,  H01L2924/01013 ,  H01L2924/01015 ,  H01L2924/01073 ,  H01L2924/14 ,  H01L2924/1433 ,  Y10T436/22

Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis.

公开(公告)号：US11982620B2

公开(公告)日：2024-05-14

申请号：US17441258

申请日：2020-03-17

Applicant: Life Technologies Corporation

Inventor： Shaohong Wang

IPC: G01N21/64 ,  G01N21/17 ,  G01N21/33

CPC classification number: G01N21/645 ,  G01N21/33 ,  G01N2021/174 ,  G01N2021/6463

Abstract: An optical detection system for a capillary electrophoresis instrument is disclosed. The system includes an ultraviolet (UV) source and an absorption measurement optical path. In an embodiment, the optical path comprises a first plurality of optical elements arranged to obtain a plurality of respective UV beamlets from a UV beam emitted by the UV source and to direct the respective UV beamlets transversely through respective capillaries of a plurality of capillaries and to an absorption detector positioned to detect respective signals for use in obtaining respective UV absorption measurements corresponding to the respective capillaries.

公开(公告)号：US11959074B2

公开(公告)日：2024-04-16

申请号：US17525737

申请日：2021-11-12

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Chiu Tai Andrew Wong ,  Kylan Szeto ,  Shanti Shankar ,  Mark Beauchemin

IPC: C12N15/10 ,  C12Q1/6825 ,  C12Q1/6874 ,  G01N35/10

CPC classification number: C12N15/1065 ,  C12Q1/6825 ,  C12Q1/6874 ,  G01N35/1081

Abstract: A method for sequencing a target polynucleotide includes detecting a first series of nucleotide incorporations complementary to at least a portion of the target polynucleotide. The first series of nucleotide incorporations forms a first complementary polynucleotide. The target nucleotide is secured to a substrate disposed in a sequencing zone of an assembly. The method further includes moving the substrate to which the target nucleotide is secured to a templating zone of the assembly; removing the first complementary polynucleotide when the substrate is disposed at the templating zone of the assembly, the target polynucleotide remaining secured to the substrate; following the removing, moving the substrate to which the target polynucleotide is secured to the sequencing zone; and detecting a second series of nucleotide incorporations complementary to at least a portion of the target polynucleotide, the second series of nucleotide incorporations forming a second complementary polynucleotide.

公开(公告)号：US20240102003A1

公开(公告)日：2024-03-28

申请号：US18345856

申请日：2023-06-30

Applicant: Thermo Fisher Scientific Baltics UAB ,  Life Technologies Corporation

Inventor： Arvydas Lubys ,  Inga Cikotiene ,  Zana Kapustina ,  Arturas Berezniakovas ,  Justina Sulgaite ,  Simona Zeimyte ,  Mark Andersen ,  Michael Allen ,  Sihong Chen

IPC: C12N15/10 ,  C12N15/115 ,  C12Q1/6806

CPC classification number: C12N15/1065 ,  C12N15/115 ,  C12Q1/6806 ,  C12N2310/3517

Abstract: The present disclosure describes oligonucleotide-tethered nucleotides, methods of making them, and methods of using them. The oligonucleotide-tethered nucleotides comprise, in some embodiments, a nucleotide linked to an oligonucleotide of from about 3 to about 100 nucleotides in length. These oligonucleotide-tethered nucleotides can be used to label a plurality of different types of nucleic acids in a plurality of different situations with a known oligonucleotide, which can serve as a barcode in some embodiments. The resulting oligonucleotide-labeled nucleic acids oligo-nucleotides can be used in a variety of nucleic acid sequencing methods.

公开(公告)号：US20240101980A1

公开(公告)日：2024-03-28

申请号：US18521466

申请日：2023-11-28

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Daniel MAZUR ,  Sihong CHEN ,  Peter VANDER HORN ,  Eileen TOZER ,  Guobin LUO ,  Joshua SHIRLEY ,  Kevin HEINEMANN

IPC: C12N9/12 ,  C12Q1/686

CPC classification number: C12N9/1252 ,  C12Q1/686 ,  C12Y207/07007

Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, recombinant polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides recombinant polymerases that yield lower systematic error rates and/or improved accuracy, when used in sequencing by synthesis reactions as compared to a control polymerase. In one aspect, the disclosure relates to recombinant polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In another aspect, the recombinant polymerases are useful for the amplification of nucleic acid templates during PCR, emPCR, isothermal amplification, recombinase polymerase amplification, rolling circle amplification, strand displacement amplification and proximity ligation amplification. In some aspects, the disclosure relates to recombinant polymerases useful for the generation of nucleic acid libraries and/or nucleic acid templates.

公开(公告)号：US20240094110A1

公开(公告)日：2024-03-21

申请号：US18240441

申请日：2023-08-31

Applicant: Life Technologies Corporation

Inventor： Michael D Ward ,  Gregory Kaduchak ,  Dmitriy Strelkov

IPC: G01N15/14

CPC classification number: G01N15/1436 ,  G01N2015/0065

Abstract: Provided are systems and methods that allow for brightfield imaging in a flow cytometer, allowing for collection of fluorescence and high-quality image date. The disclosed technology also gives rise to an illumination pattern that allows a user to create different oblique or structured illumination profiles within a static system. With the disclosed approach, a user can illuminate a sample from a first direction (e.g., with laser illumination configured to give rise to one or more of fluorescence information and scattering information), collect scattering information from a second direction, collect fluorescence information from a third direction, and capture an image of the sample from a fourth direction. (Two or more of the foregoing can be accomplished simultaneously.) Also as described elsewhere herein, an illumination used to illuminate the sample for visual image capture can be communicated to the same through a lens that also collects fluorescence from the sample.

公开(公告)号：US20240067939A1

公开(公告)日：2024-02-29

申请号：US18221099

申请日：2023-07-12

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Chieh-Yuan LI ,  David RUFF ,  Shiaw-Min CHEN ,  Jennifer O'NEIL ,  Rachel KASINSKAS ,  Jonathan ROTHBERG ,  Bin LI ,  Kai Qin LAO

IPC: C12N9/12 ,  C12Q1/6844 ,  C12Q1/6853 ,  C12Q1/6855 ,  C12Q1/686

CPC classification number: C12N9/1252 ,  C12Q1/6846 ,  C12Q1/6853 ,  C12Q1/6855 ,  C12Q1/686 ,  C12Q1/6874

Abstract: The present disclosure provides methods, compositions, kits and systems for nucleic acid amplification. In some embodiments, nucleic acid amplification methods include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, nucleic acid amplification methods include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the nucleic acid amplification method employs an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel and/or in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer which can include a sieving agent and/or a diffusion-reducing agent.

公开(公告)号：US11890557B2

公开(公告)日：2024-02-06

申请号：US17740710

申请日：2022-05-10

Applicant: Life Technologies Corporation

Inventor： Charles R. Rhodes

IPC: B01D29/27 ,  C12M1/12 ,  C12M1/00 ,  C12M1/26 ,  B01D35/027

CPC classification number: B01D29/27 ,  C12M23/14 ,  C12M23/28 ,  C12M25/16 ,  C12M29/04 ,  C12M33/14 ,  C12M47/02 ,  B01D35/027

Abstract: A filter bag assembly includes: a flexible first sheet; a flexible second sheet overlying and secured to the first sheet so that a compartment is formed therebetween; a porous filter sheet disposed between the first sheet and the second sheet so as to divide the compartment into a first compartment and a second compartment; a first port secured to the second sheet so as to communicate directly with the first compartment; and a second port secured to the second sheet so as to communicate directly with the second compartment, the second port being spaced apart from the first port. The porous filter sheet is disposed so that fluid entering the first compartment through the first port must pass through the filter sheet before entering the second compartment or exiting through the second port.

公开(公告)号：US11879156B2

公开(公告)日：2024-01-23

申请号：US17818869

申请日：2022-08-10

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Phil Waggoner ,  James A. Ball ,  Wolfgang Hinz ,  Michael L. Minto ,  Scott Parker ,  David M. Cox ,  Alexander Mastroianni ,  Jeremy Gray ,  Marc Glazer ,  Kimberly Gorrell

IPC: G01N27/414 ,  C12Q1/6869

CPC classification number: C12Q1/6869 ,  G01N27/4145 ,  C12Q1/6869 ,  C12Q2527/119 ,  C12Q2527/125 ,  C12Q2565/607

Abstract: A sensor apparatus includes a substrate, a semiconductor device disposed over the substrate, the semiconductor device having a surface electrode structure, and a saccharide coating formed over the surface electrode structure. The saccharide coating can be removed prior to use. The semiconductor device can further include a well and optionally a bead disposed in the well.