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公开(公告)号：US20170066804A1
公开(公告)日：2017-03-09
申请号：US15356818
申请日：2016-11-21
Applicant: AJINOMOTO CO., INC. , SPIBER INC.
Inventor： Kentaro Nakase
IPC: C07K14/435 , C12N15/70 , C12P21/02
CPC classification number: C07K14/43518 , C12N15/09 , C12N15/70 , C12P21/02
Abstract: A method for producing a fibroin-like protein is described. A fibroin-like protein is produced by culturing Escherichia coli having a gene encoding the fibroin-like protein in a medium, inducing expression of the gene encoding the fibroin-like protein, and collecting the fibroin-like protein, wherein the accumulation of an organic acid at the time of inducing the expression is reduced.
Abstract translation: 描述了一种生产丝心蛋白样蛋白的方法。 通过在培养基中培养具有编码丝心蛋白样蛋白质的基因的大肠杆菌，诱导编码丝心蛋白样蛋白质的基因的表达，收集丝心蛋白样蛋白质来生成丝心蛋白样蛋白质，其中有机物 诱导表达时的酸降低。
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公开(公告)号：US09023142B2
公开(公告)日：2015-05-05
申请号：US14346893
申请日：2012-10-29
Applicant: Spiber Inc.
Inventor： Junichi Sugahara , Kazuhide Sekiyama , Ryota Sato , Kaori Sekiyama , Mizuki Ishikawa , Shinya Murata , Kazuko Otomo
Abstract: A polypeptide solution of the present invention is a polypeptide solution in which a polypeptide derived from natural spider silk proteins is dissolved in a solvent. The solvent contains at least one selected from the following (i)-(iii): (i) DMSO; (ii) DMSO with an inorganic salt; and (iii) DMF with an inorganic salt. Further, in the present invention, an artificial polypeptide fiber is obtained by: using the polypeptide solution as a dope solution; and extruding the dope solution from a spinneret into a desolvation bath so as to eliminate the solvent from the dope solution and form a fiber to produce an undrawn yarn. Moreover, in the present invention, a polypeptide is purified by subjecting the polypeptide solution to heat treatment and thereafter removing an undissolved substance therefrom. Thus, the present invention provides the polypeptide solution whose solute has high solubility and solvent itself is low cost, and that allows dissolution at high temperatures and has high safety: a method for producing an artificial polypeptide fiber: and a method for purifying a polypeptide.
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公开(公告)号：US08979992B2
公开(公告)日：2015-03-17
申请号：US14346893
申请日：2012-10-29
Applicant: Spiber Inc.
Inventor： Junichi Sugahara , Kazuhide Sekiyama , Ryota Sato , Kaori Sekiyama , Mizuki Ishikawa , Shinya Murata , Kazuko Otomo
Abstract: A polypeptide solution of the present invention is a polypeptide solution in which a polypeptide derived from natural spider silk proteins is dissolved in a solvent. The solvent contains at least one selected from the following (i)-(iii): (i) DMSO; (ii) DMSO with an inorganic salt; and (iii) DMF with an inorganic salt. Further, in the present invention, an artificial polypeptide fiber is obtained by: using the polypeptide solution as a dope solution; and extruding the dope solution from a spinneret into a desolvation bath so as to eliminate the solvent from the dope solution and form a fiber to produce an undrawn yarn. Moreover, in the present invention, a polypeptide is purified by subjecting the polypeptide solution to heat treatment and thereafter removing an undissolved substance therefrom. Thus, the present invention provides the polypeptide solution whose solute has high solubility and solvent itself is low cost, and that allows dissolution at high temperatures and has high safety: a method for producing an artificial polypeptide fiber: and a method for purifying a polypeptide.
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公开(公告)号：US20250163112A1
公开(公告)日：2025-05-22
申请号：US18294011
申请日：2022-08-02
Applicant: Spiber Inc.
Inventor： Hideki Kagata , Kazuki Sakata
IPC: C07K14/435 , C08J9/26 , C08J9/28
Abstract: The present disclosure relates to a porous body containing a polypeptide derivative, in which the polypeptide derivative contains a block copolymer containing a first segment containing a polypeptide skeleton, and one or a plurality of second segments that are bonded to the first segment, the polypeptide skeleton is a recombinant polypeptide skeleton or a hydrophobic polypeptide skeleton, and the second segment contains a molecular group having a plasticizing function for the polypeptide skeleton.
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公开(公告)号：US20250075423A1
公开(公告)日：2025-03-06
申请号：US18294005
申请日：2022-08-02
Applicant: Spiber Inc.
Inventor： Hideki Kagata , Kazuki Sakata
IPC: D06N3/12 , C07K14/435 , D06N3/14 , D06N3/18
Abstract: The present disclosure relates to a synthetic leather including a base material layer containing a fiber base material, in which at least a part of the synthetic leather contains a polypeptide derivative, the polypeptide derivative contains a block copolymer containing a first segment containing a polypeptide skeleton, and one or a plurality of second segments that are bonded to the first segment, the polypeptide skeleton is a recombinant polypeptide skeleton or a hydrophobic polypeptide skeleton, and the second segment contains a molecular group having a plasticizing function for the polypeptide skeleton.
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Patent Agency Ranking
公开(公告)号：US20240270674A1
公开(公告)日：2024-08-15
申请号：US18560281
申请日：2022-05-12
Applicant: REFINE HOLDINGS Co., Ltd. , SPIBER INC.
Inventor： Tomoya FUJITA , Mitsuhiro IWAFUNE , Takao SUZUKI , Tomokiyo TAKEYAMA , Hideki ISHIZAWA , Akiyoshi ODA , Hiroaki TAKAHASHI , Junya OHNO , Kouji NAKASHIMA , Tadashi NOUMI , Hideto ISHII
IPC: C07C51/48
CPC classification number: C07C51/48
Abstract: Provided is a method for recovering a carboxylic acid, by which method the carboxylic acid is separated from an aqueous solution containing water and the carboxylic acid and the separated carboxylic acid is obtained as a carboxylic acid anhydride. The method includes a) a first step of bringing the aqueous solution into liquid-liquid contact with a prepared extractant, the prepared extractant containing a component for extracting the carboxylic acid and a diluent to dissolve the component, b) a second step of distilling the prepared extractant that is subjected to the first step, azeotropically distilling the diluent in the prepared extractant and water, separating the distillate into an extractant component layer mainly containing the diluent and an aqueous layer and then discharging the aqueous layer, returning the extractant component layer mainly containing the diluent to the distillation step as reflux, and discharging the prepared extractant containing the carboxylic acid through a bottom of the column; and c) a third step comprising re-distilling the prepared extractant containing the carboxylic acid discharged by the second step, and returning, to the first step, the prepared extractant from which the carboxylic acid and water are removed, the prepared extractant being discharged through a bottom of the column, where the diluent in the prepared extractant have a minimum boiling azeotrope with water.
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公开(公告)号：US12018405B2
公开(公告)日：2024-06-25
申请号：US16982612
申请日：2019-03-20
Applicant: SHIMA SEIKI MFG., LTD. , SPIBER INC.
Inventor： Shozo Torigoe , Seiji Shimoda , Akihiko Ozeki
IPC: D01F4/02 , C07K14/435 , D02G1/00 , D06M15/15 , D06M101/12
CPC classification number: D01F4/02 , C07K14/43518 , D02G1/00 , D06M15/15 , D06M2101/12
Abstract: A protein fiber having a crimp property, such that the protein fiber crimps in response to a stimulus, is immersed in a solution of protein having a different composition from the protein fiber. The protein fiber treated with the different protein composition is infiltrated into the protein and the protein fiber is made to crimp.
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公开(公告)号：US20230128695A1
公开(公告)日：2023-04-27
申请号：US17911296
申请日：2021-03-16
Applicant: Spiber Inc.
Inventor： Kazuki Sakata , Nao Kitahara , Seiji Shirakawa , Kentaro Takahashi , Leona Muto , Oliver Syed Shafaat , Hideki Kagata
Abstract: An aspect of the present disclosure provides a synthetic polymer containing a first segment containing a polypeptide skeleton and one or a plurality of second segments bonded directly to the first segment, in which the second segment contains a molecular group having a plasticizing function for the polypeptide skeleton.
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公开(公告)号：US20230039765A1
公开(公告)日：2023-02-09
申请号：US17762912
申请日：2020-09-25
Applicant: Spiber Inc.
Inventor： Hideto Ishii , Mizuki Igarashi , Tatsuki Adachi , Yunosuke Abe
IPC: C07K14/435 , D02J1/22 , D01F4/02 , D01D5/06
Abstract: An object of the present invention is to provide a method capable of producing a protein fiber in which shrinkage at the time of first contact with moisture after production is more reliably suppressed and generation of crimp is also suppressed. A method for producing a protein fiber according to the present invention includes: a pre-shrinking step of shrinking a protein fibril in a relaxed state after drawing and before being wound; a winding step of winding the protein fibril subjected to the pre-shrinking step to obtain a wound product; and a post-shrinking step of shrinking the protein fibril subjected to the winding step in a relaxed state.
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公开(公告)号：US20220378871A1
公开(公告)日：2022-12-01
申请号：US17765342
申请日：2020-09-30
Applicant: Spiber Inc. , National University Corporation Tokai National Higher Education and Research System
Inventor： Hiroo Uchida , Hizuru Nagata , Akinari Hinoki , Masataka Fujii , Yun-Hsiang Chang , Satoshi Harada , Takehiro Sato
Abstract: To provide a muscle tissue-regenerating agent containing a fibroin protein.
A muscle tissue-regenerating agent containing a modified fibroin protein.
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