公开(公告)号：US20200207817A1

公开(公告)日：2020-07-02

申请号：US16633589

申请日：2018-07-25

Applicant: Spiber Inc. ,  Riken

Inventor： Keisuke Morita ,  Yunosuke Abe ,  Takehisa Maekawa ,  Koichi Kotaka ,  Junichi Sugahara ,  Keiji Numata

IPC: C07K14/435 ,  D01F4/02 ,  C12N15/63

Abstract: The present invention relates to a modified fibroin including a domain sequence represented by Formula 1: [(A)n motif-REP]m or Formula 2: [(A)n motif-REP]m-(A)n motif, in which the domain sequence has an amino acid sequence with a reduced content of a glutamine residue equivalent to an amino acid in which one or a plurality of glutamine residues in REP are deleted or substituted with other amino acid residues, as compared with a naturally occurring fibroin. [In Formula 1 and Formula 2, (A)n motif represents an amino acid sequence consisting of 4 to 27 amino acid residues, and the number of alanine residues with respect to the total number of amino acid residues in the (A)n motif is 80% or more. REP represents an amino acid sequence consisting of 10 to 200 amino acid residues. m represents an integer of 10 to 300. A plurality of (A)n motifs may be the same amino acid sequence or different amino acid sequences.]

公开(公告)号：US20210340193A1

公开(公告)日：2021-11-04

申请号：US17280125

申请日：2019-09-30

Applicant: Spiber Inc.

Inventor： Tetsuo Asakura ,  Yunosuke Abe ,  Hideto Ishii

IPC: C07K14/435 ,  D01F4/02

Abstract: A production method of a protein composition, the method comprising a step of hydrolyzing an ester group by bringing a raw material composition containing an esterified protein into contact with an acidic or basic medium.

公开(公告)号：US20210040648A1

公开(公告)日：2021-02-11

申请号：US16965626

申请日：2019-01-31

Applicant: Spiber Inc.

Inventor： Yunosuke Abe ,  Hirotada Ando

IPC: D01F4/02 ,  D01D5/06

Abstract: The present invention relates to a method for manufacturing a protein fiber, the method including a step of bringing a spinning raw liquid containing a spider silk protein and a solvent into contact with a coagulation liquid to coagulate the spider silk protein, in which the coagulation liquid contains a ketone.

公开(公告)号：US20230039765A1

公开(公告)日：2023-02-09

申请号：US17762912

申请日：2020-09-25

Applicant: Spiber Inc.

Inventor： Hideto Ishii ,  Mizuki Igarashi ,  Tatsuki Adachi ,  Yunosuke Abe

IPC: C07K14/435 ,  D02J1/22 ,  D01F4/02 ,  D01D5/06

Abstract: An object of the present invention is to provide a method capable of producing a protein fiber in which shrinkage at the time of first contact with moisture after production is more reliably suppressed and generation of crimp is also suppressed. A method for producing a protein fiber according to the present invention includes: a pre-shrinking step of shrinking a protein fibril in a relaxed state after drawing and before being wound; a winding step of winding the protein fibril subjected to the pre-shrinking step to obtain a wound product; and a post-shrinking step of shrinking the protein fibril subjected to the winding step in a relaxed state.

公开(公告)号：US20210214404A1

公开(公告)日：2021-07-15

申请号：US17262350

申请日：2019-07-24

Applicant: Spiber Inc. ,  Kojima Industries Corporation

Inventor： Masato Matsuo ,  Hideto Ishii ,  Yunosuke Abe

IPC: C07K14/435 ,  A41G3/00

Abstract: The present invention pertains to an artificial hair fiber that includes an artificial fibroin fiber containing a modified fibroin and that expands when being in a wet state and shrinks when being dried after the wet state.

公开(公告)号：US20210040649A1

公开(公告)日：2021-02-11

申请号：US16965661

申请日：2019-01-31

Applicant: Spiber Inc.

Inventor： Akito Sato ,  Yunosuke Abe

IPC: D01F4/02 ,  D02G3/24 ,  D06M11/05

Abstract: An object of the present invention is to provide a method for efficiently manufacturing a protein crimped staple from protein filaments at a low cost. A manufacturing method for a protein crimped staple according to the present invention includes: a) preparing an artificial fibroin filament containing a modified fibroin; b) cutting the artificial fibroin filament to obtain an artificial fibroin staple; and c) performing crimping by bringing the artificial fibroin filament into contact with an aqueous medium to crimp the artificial fibroin filament before the cutting or bringing the artificial fibroin staple into contact with an aqueous medium to crimp the artificial fibroin staple after the cutting.

公开(公告)号：US12145971B2

公开(公告)日：2024-11-19

申请号：US16611315

申请日：2018-05-09

Applicant: Spiber Inc. ,  Kojima Industries Corporation

Inventor： Ayumi Abe ,  Keisuke Morita ,  Kenji Kurachi ,  Yunosuke Abe

IPC: C07K14/47 ,  D01D5/06 ,  D01F4/00

Abstract: The present invention relates to a polypeptide solution in which a polypeptide including an amino acid sequence derived from type I keratin that belongs to Cluster Ia is dissolved in a solvent including a formic acid or a solvent including an aprotic polar agent having a dipole moment of 3.0 D or more and an inorganic salt.

公开(公告)号：US20220324923A1

公开(公告)日：2022-10-13

申请号：US17764669

申请日：2020-09-28

Applicant: Spiber Inc.

Inventor： Kentaro Takahashi ,  Atsushi Ikeda ,  Hideki Kagata ,  Yunosuke Abe ,  Kazuki Sakata

IPC: C07K14/435 ,  D01F4/02

Abstract: An object of the present invention is to provide a method for advantageously producing a protein molded article which can solve a problem caused by esterification of a hydroxyl group contained in a protein while maintaining a sufficient strength. A method for producing a protein molded article according to the present invention includes a step of bringing a raw material molded article containing a protein in which a hydroxyl group is esterified into contact with an acidic or basic medium in a state of applying a tensile force, thus hydrolyzing an ester group.

公开(公告)号：US20220127755A1

公开(公告)日：2022-04-28

申请号：US17428136

申请日：2020-02-07

Applicant: Spiber Inc.

Inventor： Hirotada Ando ,  Hideto Ishii ,  Koichi Kotaka ,  Ryoko Sato ,  Hiroshi Kano ,  Jun Kobayashi ,  Akito Sato ,  Yunosuke Abe

IPC: D01D5/06 ,  C07K14/435 ,  D01F4/02

Abstract: An object of the present invention is to provide a method for producing an artificial structural protein fiber having a small diameter and having a stress equal to or higher than that of the related art. A method for producing an artificial structural protein fiber according to the present invention is a method for producing an artificial structural protein fiber by a wet spinning method, the method including a coagulation step of discharging a spinning dope containing an artificial structural protein and an organic solvent from a spinneret into a coagulation liquid to coagulate the artificial structural protein, wherein a bath draft in the coagulation step is more than 0.4 and 20 or less.

公开(公告)号：US20200165311A1

公开(公告)日：2020-05-28

申请号：US16611315

申请日：2018-05-09

Applicant: Spiber Inc. ,  Kojima Industries Corporation

Inventor： Ayumi Abe ,  Keisuke Morita ,  Kenji Kurachi ,  Yunosuke Abe

IPC: C07K14/47 ,  C08L89/00 ,  D01F4/00 ,  D01D5/06

Abstract: The present invention relates to a polypeptide solution in which polypeptide including an amino acid sequence derived from type I keratin that belongs to Cluster Ia is dissolved in a solvent including a formic acid or a solvent including an aprotic polar agent having a dipole moment of 3.0 D or more and an inorganic salt.