Abstract:
The present application provides methods for obtaining single cells from a sample. Methods for isolating and analyzing molecular features obtained from a single cell are also disclosed herein. For example, individual circulating tumor cells (CTCs) from a sample such as a patient's blood sample can be identified and obtained using methods disclosed herein, and picked for further analysis.
Abstract:
A diaphragm air pump comprises a pump chamber, a diaphragm and one or more piezoelectric beams or bimorphs. Fluid flows into the pump chamber and then flows out of it, the diaphragm is provided within the pump chamber, and one or more central openings are formed in the diaphragm. One or more central check valves are provided in the central openings. The diaphragm is just bonded with piezoelectric beams, not fixed to the lower housing of the pump chamber in order to get large displacement. With the provided diaphragm air pump, it is possible to actively adjust the air quantity according to the requirement for fuel cell or a part-to-be-cooled, and it is also possible to reduce noise and power consumption compared with a conventional fan type cooler or air pumps.
Abstract:
The present disclosure relates to methods of quantifying nucleic acid molecules immobilized on an electrode of an electrochemical assay chip. Such quantification can be used in testing and quality control of assay chips. Materials and instrumentation useful in performing such techniques are also disclosed.
Abstract:
A method of estimating timing of at least one of the beginning and the end of a transmitted signal segment in the presence of time delay in a signal transmission channel. Each of a sequence of signal frames is provided with a pseudo-noise (PN) m-sequences, where the PN sequences satisfy selected orthogonality and closures relations. A convolution signal is formed between a received signal and the sequence of PN segments and is subtracted from the received signal to identify the beginning and/or end of a PN segment within the received signal. PN sequences are used for timing recovery, for carrier frequency recovery, for estimation of transmission channel characteristics, for synchronization of received signal frames, and as a replacement for guard intervals in an OFDM context.
Abstract:
A method of modifying a surface of a casting involves providing a casting mould; placing a perforated mask with the mould to define a masked area of the mould; spray-coating the masked area of the mould with a coating material selected for forming a surface layer on the casting; introducing a liquid casting material to the mould; and, solidifying the liquid casting material to form a surface modified casting. The present method reduces the tendency for the coating material to spall from the mould and permits the formation of thicker coating layers on the mould. Thicker and better quality surface layers may be formed on castings.
Abstract:
An anti stiction structure for cantilever formation technique. In one embodiment, the polymer cantilever is prevented from sticking to the substrate by at amortized stick layer on the substrate during formation that is later removed as a sacrificial layer. In another embodiment, the cantilever includes downwardly extending legs.
Abstract:
A micromachined fluid handling device having improved properties. The valve is made of reinforced parylene. A heater heats a fluid to expand the fluid. The heater is formed on unsupported silicon nitride to reduce the power. The device can be used to form a valve or a pump. Another embodiment forms a composite silicone/parylene membrane. Another feature uses a valve seat that has concentric grooves for better sealing operation.