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    Bead manipulation techniques
    43.
    发明授权
    Bead manipulation techniques 有权
    珠操纵技术

    公开(公告)号：US09050606B2

    公开(公告)日：2015-06-09

    申请号：US14491231

    申请日：2014-09-19

    Applicant: ADVANCED LIQUID LOGIC, INC.

    Inventor： Vamsee K. Pamula ,  Arjun Sudarsan ,  Ramakrishna Sista

    IPC: G01N15/06 ,  G01N33/00 ,  G01N33/48 ,  B03C5/00 ,  G01N33/543 ,  B03C1/01 ,  B03C1/28 ,  B03C5/02

    CPC classification number: B03C7/026 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0887 ,  B01L2400/0424 ,  B01L2400/0427 ,  B03C1/01 ,  B03C1/288 ,  B03C5/00 ,  B03C5/005 ,  B03C5/026 ,  B03C2201/26 ,  G01N33/54326

    Abstract: The invention provides a method of redistributing magnetically responsive beads in a droplet. The method may include conducting on a droplet operations surface one or more droplet operations using the droplet without removing the magnetically responsive beads from the region of the magnetic field. The droplet operations may in some cases be electrode-mediated. The droplet operations may redistribute and/or circulate the magnetically responsive beads within the droplet. In some cases, the droplet may include a sample droplet may include a target analyte. The redistributing of the magnetically responsive beads may cause target analyte to bind to the magnetically responsive beads. In some cases, the droplet may include unbound substances in a wash buffer. The redistributing of the magnetically responsive beads causes unbound substances to be freed from interstices of an aggregated set or subset of the magnetically responsive beads.

    Abstract translation: 本发明提供了一种在液滴中重新分布磁响应珠的方法。 该方法可以包括在液滴操作表面上使用液滴进行一个或多个液滴操作，而不从磁场的区域移除磁响应珠。 在某些情况下，液滴操作可能是电极介导的。 液滴操作可以在液滴内重新分布和/或循环磁响应珠。 在一些情况下，液滴可以包括样品液滴可以包括靶分析物。 磁响应珠的重新分布可能导致目标分析物结合磁响应珠。 在一些情况下，液滴可以包括洗涤缓冲液中的未结合物质。 磁响应珠子的重新分布导致未结合物质从磁性响应珠粒的聚集或子集的空隙中释放出来。

    Droplet Dispensing Device and Methods
    44.
    发明申请
    Droplet Dispensing Device and Methods 审中-公开
    滴液分配装置及方法

    公开(公告)号：US20150075985A1

    公开(公告)日：2015-03-19

    申请号：US14541825

    申请日：2014-11-14

    Applicant: ADVANCED LIQUID LOGIC, INC.

    Inventor： Michael G. Pollack ,  Vamsee K. Pamula ,  Vijay Srinivasan

    IPC: B01L3/00 ,  G01N27/447

    CPC classification number: B01L3/502761 ,  B01F13/0071 ,  B01F13/0076 ,  B01L3/502792 ,  B01L2200/0605 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2200/16 ,  B01L2300/0819 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/088 ,  B01L2300/089 ,  B01L2400/0427 ,  B01L2400/043 ,  G01N27/447

    Abstract: The invention provides nonlimiting examples of structures for and methods of dispensing droplets in a droplet actuator. The droplet actuator structures and methods of the invention exhibit numerous advantages over droplet actuators of the prior art. In various embodiments, the structures and methods of the invention provide, among other things, improved efficiency, throughput, scalability, and/or droplet uniformity, as compared with existing droplet actuators. Further, in some embodiments, the droplet actuators provide configurations for improved methods of loading and/or unloading fluid and/or droplets. In yet other embodiments, the droplet actuators provide fluid loading configurations for loading numerous fluid reservoirs in a substantially simultaneous and/or substantially sequential manner.

    Abstract translation: 本发明提供了用于在液滴致动器中分配液滴的结构的方法和方法的非限制性实例。 本发明的液滴致动器结构和方法与现有技术的液滴致动器相比表现出许多优点。 在各种实施例中，与现有的液滴致动器相比，本发明的结构和方法尤其提供了改进的效率，吞吐量，可扩展性和/或液滴均匀性。 此外，在一些实施例中，液滴致动器提供用于加载和/或卸载流体和/或液滴的改进方法的构造。 在其他实施例中，液滴致动器提供用于以基本上同时和/或基本顺序的方式装载多个流体储存器的流体加载构造。

    DROPLET-BASED AFFINITY ASSAYS
    46.
    发明申请
    DROPLET-BASED AFFINITY ASSAYS 有权
    基于DROPLET的亲和力测定

    公开(公告)号：US20130252262A1

    公开(公告)日：2013-09-26

    申请号：US13890715

    申请日：2013-05-09

    Applicant: ADVANCED LIQUID LOGIC INC.

    Inventor： Vijay Srinivasan ,  Vamsee K. Pamula ,  Michael G. Pollack ,  Richard B. Fair

    IPC: G01N27/72

    CPC classification number: G01N27/72 ,  G01N27/745 ,  G01N33/54326

    Abstract: A method of detecting a target analyte in a sample, including executing electrowetting-mediated droplet operations and thereby: combining one or more immunoassay reagent droplets comprising magnetically-responsive beads having affinity for the target analyte with one or more sample droplets potentially comprising the target analyte to yield a first combined droplet; beginning with the combined droplet, effecting a droplet-based washing protocol to wash the magnetically-responsive beads to yield a first washed droplet comprising the washed magnetically responsive beads; and combining the droplet comprising the washed magnetically responsive beads with a droplet comprising a reporter molecule having affinity for target analyte captured on the magnetically-responsive beads to yield a second combined droplet; beginning with the second combined droplet, effecting a droplet-based washing protocol to wash the magnetically-responsive beads to yield a second washed droplet comprising the washed magnetically responsive beads; detecting a signal from the second washed droplet which corresponds to the presence, absence and/or quantity of the analyte in the sample.

    Abstract translation: 一种检测样品中的目标分析物的方法，包括执行电润湿介导的液滴操作，从而：将包含对靶分析物具有亲和性的磁响应珠粒的一种或多种免疫测定试剂液滴与潜在地包含靶分析物的一个或多个样品液 以产生第一组合液滴; 从组合的液滴开始，实现基于液滴的洗涤方案以洗涤磁响应珠，以产生包含洗涤的磁响应珠的第一洗涤液滴; 以及将包含洗涤的磁响应珠粒的液滴与包含对在磁响应珠上捕获的靶分析物具有亲和力的报道分子的液滴组合以产生第二组合液滴; 从第二组合液滴开始，实现基于液滴的洗涤方案以洗涤磁响应珠，产生包含洗涤的磁响应珠的第二洗涤液滴; 检测来自第二洗涤液滴的信号，其对应于样品中分析物的存在，不存在和/或数量。

    Capacitance detection in a droplet actuator
    48.
    发明授权
    Capacitance detection in a droplet actuator 有权

    公开(公告)号：US10183292B2

    公开(公告)日：2019-01-22

    申请号：US14968094

    申请日：2015-12-14

    Applicant: Advanced Liquid Logic, Inc.

    Inventor： Ryan A. Sturmer ,  Michael G. Pollack ,  Vamsee K. Pamula ,  Vijay Srinivasan ,  Philip Y. Paik

    IPC: B01L3/00 ,  G01N27/22 ,  G01V3/02

    Abstract: A method, circuit and apparatus for detecting capacitance on a droplet actuator, inter alia, for determining the presence, partial presence or absence of a droplet at an electrode on a droplet actuator by: (a) providing a droplet actuator comprising: (i) a substrate comprising electrodes arranged on the substrate for conducting droplet operations on a surface of the substrate; (ii) a capacitance detection circuit for detecting capacitance at the droplet operations surface at one or more of the electrodes; (b) detecting capacitance at the droplet operations surface at one or more of the electrodes; and (c) determining from the capacitance the presence, partial presence or absence of a droplet at the droplet operations surface at the electrode.

    Bead Manipulation Techniques
    50.
    发明申请
    Bead Manipulation Techniques 审中-公开
    珠操作技术

    公开(公告)号：US20160271623A1

    公开(公告)日：2016-09-22

    申请号：US15165883

    申请日：2016-05-26

    Applicant: ADVANCED LIQUID LOGIC, INC.

    Inventor： Vamsee K. Pamula ,  Prasanna Thwar ,  Arjun Sudarsan ,  Ramakrishna Sista

    IPC: B03C7/02 ,  B01L3/00

    CPC classification number: B03C7/026 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0887 ,  B01L2400/0424 ,  B01L2400/0427 ,  B03C1/01 ,  B03C1/288 ,  B03C5/00 ,  B03C5/005 ,  B03C5/026 ,  B03C2201/26 ,  G01N33/54326

    Abstract: The invention provides a method of redistributing magnetically responsive beads in a droplet. The method may include conducting on a droplet operations surface one or more droplet operations using the droplet without removing the magnetically responsive beads from the region of the magnetic field. The droplet operations may in some cases be electrode-mediated. The droplet operations may redistribute and/or circulate the magnetically responsive beads within the droplet. In some cases, the droplet may include a sample droplet may include a target analyte. The redistributing of the magnetically responsive beads may cause target analyte to bind to the magnetically responsive beads. In some cases, the droplet may include unbound substances in a wash buffer. The redistributing of the magnetically responsive beads causes unbound substances to be freed from interstices of an aggregated set or subset of the magnetically responsive beads.

    Abstract translation: 本发明提供了一种在液滴中重新分布磁响应珠的方法。 该方法可以包括在液滴操作表面上使用液滴进行一个或多个液滴操作，而不从磁场的区域移除磁响应珠。 在某些情况下，液滴操作可能是电极介导的。 液滴操作可以在液滴内重新分布和/或循环磁响应珠。 在一些情况下，液滴可以包括样品液滴可以包括靶分析物。 磁响应珠的重新分布可能导致目标分析物结合磁响应珠。 在一些情况下，液滴可以包括洗涤缓冲液中的未结合物质。 磁响应珠子的重新分布导致未结合物质从磁性响应珠粒的聚集或子集的空隙中释放出来。

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