公开(公告)号：US11254968B2

公开(公告)日：2022-02-22

申请号：US15688250

申请日：2017-08-28

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Jonathan William Larson ,  Qun Zhong ,  Darren Roy Link

IPC: C12Q1/68 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/686 ,  C12Q1/6883 ,  C12Q1/6886

Abstract: The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.

公开(公告)号：US10761090B2

公开(公告)日：2020-09-01

申请号：US15415276

申请日：2017-01-25

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Michael L. Samuels ,  Darren Roy Link

IPC: G01N33/50 ,  G01N33/543 ,  C40B40/04 ,  C40B70/00 ,  G01N33/532 ,  C40B40/00 ,  C12Q1/6874 ,  B01F13/00

Abstract: The invention generally relates to performing sandwich assays in droplets. In certain embodiments, the invention provides methods for detecting a target analyte that involve forming a compartmentalized portion of fluid including a portion of a sample suspected of containing a target analyte and a sample identifier, a first binding agent having a target identifier, and a second binding agent specific to the target analyte under conditions that produce a complex of the first and second binding agents with the target analyte, separating the complexes, and detecting the complexes, thereby detecting the target analyte.

公开(公告)号：US10351905B2

公开(公告)日：2019-07-16

申请号：US13866111

申请日：2013-04-19

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Darren Roy Link ,  Benjamin J. Miller

IPC: C12Q1/68 ,  C12Q1/686

Abstract: The invention generally relates to detecting target molecules.