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    Biomass fractionation process for bioproducts
    51.
    发明授权
    Biomass fractionation process for bioproducts 有权
    生物产品的生物质分馏过程

    公开(公告)号：US09580454B2

    公开(公告)日：2017-02-28

    申请号：US12926323

    申请日：2010-11-10

    Applicant: Zhirun Yuan ,  Thomas Carl Browne ,  Xiao Zhang

    Inventor： Zhirun Yuan ,  Thomas Carl Browne ,  Xiao Zhang

    IPC: C07G1/00 ,  C08H8/00 ,  C12P7/10 ,  C12P17/06 ,  C12P19/04 ,  C12P19/14 ,  C13K1/02

    CPC classification number: C12P19/14 ,  C07G1/00 ,  C08H8/00 ,  C12P7/10 ,  C12P17/06 ,  C12P19/02 ,  C12P19/04 ,  C12P2201/00 ,  C13K1/02 ,  Y02E50/16

    Abstract: A fractionation process for producing value-added products from a lignocellulosic biomass, comprises: a) mechanically refining the lignocellulosic biomass under mild refining conditions to form a refined biomass pulp with enhanced susceptibility to separation of hemicellulose, cellulose and lignin, and enhanced digestibility of carbohydrates in hydrolysis b) separating hemicellulose and sulfur-free high-quality lignin from cellulose in the refined biomass, and, optionally c) producing various bioproducts from the above said process.

    Abstract translation: 用于从木质纤维素生物质生产增值产物的分馏方法包括：a）在温和的澄清条件下机械地提炼木质纤维素生物质以形成具有增加的半纤维素，纤维素和木质素分离敏感性的精制生物质纸浆，以及提高碳水化合物的消化率 在水解中b）从精制生物质中的纤维素分离半纤维素和无硫的高品质木质素，以及任选地c）从上述方法生产各种生物产物。

    Useful halophilic, thermostable and ionic liquids tolerant cellulases
    52.
    发明申请
    Useful halophilic, thermostable and ionic liquids tolerant cellulases 有权
    有用的嗜盐，耐热和离子液体耐受性纤维素酶

    公开(公告)号：US20170029856A1

    公开(公告)日：2017-02-02

    申请号：US15164813

    申请日：2016-05-25

    Applicant: The Regents of the University of California ,  Sandia Corporation

    Inventor： Tao Zhang ,  Supratim Datta ,  Blake A. Simmons ,  Edward M. Rubin

    IPC: C12P19/14 ,  C12P19/02

    CPC classification number: C12P19/14 ,  C12N9/2437 ,  C12P19/02 ,  C12P2201/00 ,  C12P2203/00 ,  C12Y302/01091 ,  Y02P20/52

    Abstract: The present invention provides for an isolated or recombinant polypeptide comprising an amino acid sequence having at least 70% identity with the amino acid sequence of a Halorhabdus utahensis cellulase, such as Hu-CBH1, wherein said amino acid sequence has a halophilic thermostable and/or thermophilic cellobiohydrolase (CBH) activity. In some embodiments, the polypeptide has a CBH activity that is resistant to up to about 20% of ionic liquids. The present invention also provides for compositions comprising and methods using the isolated or recombinant polypeptide.

    Abstract translation: 本发明提供了一种分离的或重组的多肽，其包含与乌尔氏假单胞菌纤维素酶如Hu-CBH1的氨基酸序列具有至少70％同一性的氨基酸序列，其中所述氨基酸序列具有嗜盐热稳定剂和/或 嗜热纤维二糖水解酶（CBH）活性。 在一些实施方案中，多肽具有耐高达约20％离子液体的CBH活性。 本发明还提供包含使用分离或重组多肽的方法的组合物。

    Method and system for processing biomass
    54.
    发明授权
    Method and system for processing biomass 有权
    处理生物质的方法和系统

    公开(公告)号：US09546351B2

    公开(公告)日：2017-01-17

    申请号：US12758291

    申请日：2010-04-12

    Applicant: Fernando Roberto Paz Briz ,  Fernando Roberto Paz Alcazar

    Inventor： Fernando Roberto Paz Briz ,  Fernando Roberto Paz Alcazar

    IPC: C12M1/00

    CPC classification number: C12M45/04 ,  C12P7/10 ,  C12P2201/00 ,  C12P2203/00 ,  Y02E50/16

    Abstract: A method and system for processing biomass. The method uses an apparatus with a housing, a rotor inside of the housing, and a plurality of protrusions extending from the rotor. The method includes placing the biomass in a fluid medium, inducing cavitation within the fluid to partially separate the biomass, sending the biomass and fluid into the housing, rotating the rotor to further separate the biomass, heating the biomass and fluid, and maintaining the acidity of the fluid and biomass at a pH of between approximately 2 to 6. The system includes apparatus each having a housing and a rotor within the housing. One apparatus has abutting, alternating height protrusions extending from the rotor. Another apparatus has grooves formed in the rotor and an end wall of the housing. Another apparatus has two rows of protrusions extending from the rotor that are spaced apart no less than approximately 6 millimeters.

    Abstract translation: 一种处理生物质的方法和系统。 该方法使用具有壳体，壳体内的转子和从转子延伸的多个突起的装置。 该方法包括将生物量放置在流体介质中，在流体内引入气穴以部分地分离生物质，将生物质和流体发送到壳体中，旋转转子以进一步分离生物质，加热生物质和流体，并保持酸度 在约2至6之间的pH下的流体和生物质。该系统包括每个在壳体内具有壳体和转子的装置。 一个装置具有从转子延伸的邻接的交替的高度突起。 另一种装置具有形成在转子中的凹槽和壳体的端壁。 另一装置具有从转子延伸的两排突起，其间隔开不小于约6毫米。

    BIOMASS TRANSFORMATION
    55.
    发明申请
    BIOMASS TRANSFORMATION 审中-公开
    生物量转换

    公开(公告)号：US20170009257A1

    公开(公告)日：2017-01-12

    申请号：US15117742

    申请日：2015-02-13

    Applicant: DEINOVE

    Inventor： FABIEN COZE ,  NAGIB WARD ,  ASSIA DREUXZIGHA ,  CLARINE LAZZARELLI ,  JEAN-PAUL LEONETTI

    IPC: C12P1/04 ,  C12P7/04

    CPC classification number: C12P1/04 ,  C12P7/04 ,  C12P7/065 ,  C12P7/10 ,  C12P2201/00 ,  Y02E50/16 ,  Y02E50/17

    Abstract: The present invention provides improved methods of transforming biomass using Deinococcus bacteria. More particularly, the invention discloses improved methods that combine suitable biomass treatment and processing conditions, allowing transformation of biomass and generation of valuable products under industrially-effective conditions

    Abstract translation: 本发明提供了使用灭球菌转化生物质的改进方法。 更具体地，本发明公开了将合适的生物质处理和处理条件相结合的改进方法，允许在工业上有效的条件下转化生物质和产生有价值的产物

    Method for the simultaneous production of ethanol and a fermented, solid product
    57.
    发明授权
    Method for the simultaneous production of ethanol and a fermented, solid product 有权
    同时生产乙醇和发酵固体产品的方法

    公开(公告)号：US09528128B2

    公开(公告)日：2016-12-27

    申请号：US14349170

    申请日：2012-10-04

    Applicant: Hamlet Protein A/S

    Inventor： Ole Kaae Hansen ,  Katrine Hvid Ellegard ,  Karl Kristian Thomsen

    IPC: A61K8/60 ,  C13K13/00 ,  C12P7/06 ,  C12P7/10 ,  C13K1/02 ,  C12P19/02 ,  C12P19/14 ,  C12P1/02 ,  C12P7/08 ,  A61K8/64 ,  A61K47/36 ,  A61K47/42

    CPC classification number: C12P7/06 ,  A23K10/12 ,  A23K10/38 ,  A23L33/10 ,  A23L33/185 ,  A23V2002/00 ,  A61K8/60 ,  A61K8/645 ,  A61K47/36 ,  A61K47/42 ,  C12P1/02 ,  C12P7/08 ,  C12P7/10 ,  C12P19/02 ,  C12P19/14 ,  C12P21/00 ,  C12P2201/00 ,  C12P2203/00 ,  C12Y302/01 ,  C12Y302/01001 ,  C12Y302/01003 ,  C12Y302/01022 ,  C13K1/02 ,  C13K13/00 ,  Y02E50/16 ,  Y02E50/17 ,  Y02P60/873

    Abstract: The invention relates to a method for the simultaneous production of a fermented, solid product and ethanol comprising the following steps: 1) providing a mixture of milled or flaked or otherwise disintegrated biomass, comprising oligosaccharides and/or polysaccharides and live yeast in a dry matter ratio of from 2:1 to 100:1, and water; 2) fermenting the mixture resulting from step (1) under conditions where the water content in the initial mixture does not exceed 65% by weight, for 1-36 hours at a temperature of about 25-60° C. under anaerobic conditions; 3) incubating the fermented mixture resulting from step (2) for 0.5-240 minutes at a temperature of about 70-150° C.; and 4) separating wet fermented, solid product from the fermented mixture resulting from step (3); further comprising either a) that the fermentation in step (2) is performed in one or more interconnected paddle worm or continuous worm conveyers with inlet means for the fermentation mixture and additives and outlet means for the ferment as well as control means for rotation speed, temperature and pH, or b) that one or more processing aids are added in any of steps (1), (2) and (3) and further comprising a step of 5) separating crude ethanol from the fermented mixture in step (2) by vacuum and/or in step (3) by vacuum or by injection of steam and condensing the surplus stripping steam. The invention further relates to the products of this method as well as uses thereof.

    Abstract translation: 本发明涉及同时生产发酵的固体产物和乙醇的方法，包括以下步骤：1）提供在干物质中包含低聚糖和/或多糖和活酵母的经研磨或片状或其他崩解的生物质的混合物 比例为2：1至100：1，和水; 2）在厌氧条件下，在约25-60℃的温度下，在初始混合物中的水含量不超过65重量％的条件下，将步骤（1）产生的混合物发酵1-36小时; 3）在约70-150℃的温度下将步骤（2）得到的发酵混合物温育0.5-240分钟; 和4）从步骤（3）得到的发酵混合物中分离出湿发酵的固体产物; 进一步包括a）步骤（2）中的发酵在一个或多个相互连接的桨式蜗杆或连续的蜗杆传送器中进行，其具有用于发酵混合物的入口装置和用于发酵的添加剂和出口装置以及用于转速的控制装置， （1），（2）和（3）中任一步骤中加入一种或多种加工助剂，还包括步骤（5）在步骤（2）中从发酵混合物中分离粗乙醇， 通过真空和/或在步骤（3）中通过真空或通过注入蒸汽并冷凝多余的汽提蒸汽。 本发明还涉及该方法的产物及其用途。

    HEAT-STABLE, FE-DEPENDENT ALCOHOL DEHYDROGENASE FOR ALDEHYDE DETOXIFICATION
    59.
    发明申请
    HEAT-STABLE, FE-DEPENDENT ALCOHOL DEHYDROGENASE FOR ALDEHYDE DETOXIFICATION 审中-公开
    适用于ALDEHYDE脱氧的热稳定性酒精脱氢酶

    公开(公告)号：US20160340702A1

    公开(公告)日：2016-11-24

    申请号：US15060078

    申请日：2016-03-03

    Applicant: UT-BATTELLE, LLC

    Inventor： James G. Elkins ,  Sonya Clarkson

    IPC: C12P17/04 ,  C12P7/10

    CPC classification number: C12P17/04 ,  C12N9/0006 ,  C12P7/10 ,  C12P2201/00 ,  C12Y101/01002 ,  Y02E50/16

    Abstract: The present invention relates to microorganisms and polypeptides for detoxifying aldehydes associated with industrial fermentations. In particular, a heat-stable, NADPH- and iron-dependent alcohol dehydrogenase was cloned from Thermoanaerobacter pseudethanolicus 39E and displayed activity against a number of aldehydes including inhibitory compounds that are produced during the dilute-acid pretreatment process of lignocellulosic biomass before fermentation to biofuels. Methods to use the microorganisms and polypeptides of the invention for improved conversion of bio mass to biofuel are provided as well as use of the enzyme in metabolic engineering strategies for producing longer-chain alcohols from sugars using thermophilic, fermentative microorganisms.

    Abstract translation: 本发明涉及用于解毒与工业发酵相关的醛的微生物和多肽。 特别地，从Thermoanerobacter pseudethanolicus 39E克隆了热稳定的NADPH-和铁依赖性醇脱氢酶，并显示出对许多醛的活性，包括在发酵到生物燃料之前在木质纤维素生物质的稀酸预处理过程中产生的抑制性化合物 。 提供了使用本发明的微生物和多肽来改善生物质量转化为生物燃料的方法，以及在代谢工程策略中使用该酶从使用嗜热发酵微生物的糖生产长链醇的方法。

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