公开(公告)号：US5325295A

公开(公告)日：1994-06-28

申请号：US688220

申请日：1991-04-22

Applicant: Joseph C. Fratantoni ,  Betty J. Poindexter

Inventor： Joseph C. Fratantoni ,  Betty J. Poindexter

IPC: G01N15/00 ,  G01N21/82 ,  G06F15/00 ,  G01N33/48

CPC classification number: G01N21/82 ,  G01N2015/0084 ,  G01N2201/0407 ,  G01N2201/0446

Abstract: A rapid method for the simultaneous measurement of aggregation or agglutination in plurality of microtest wells or other sample-holding vessels comprises providing a plurality of light beams having discrete focal points adapted to be focused on the contents of a plurality of wells or other vessels and a plurality of photodetectors therefor, directing the light beams through the wells or other vessels and contents thereof, continuously agitating the wells or other vessels and contents thereof with a circular movement effective to permit aggregation or agglutination while preserving the integrity of the material, producing a plurality of output signals from the detectors are proportional to the light absorbencies of the beams as they pass through the wells or other vessels and contents thereof, repeatedly sampling the signals at a predetermined time interval and determining changes in optical densities from the signals obtained after each predetermined time interval by comparing each signal sampled after each predetermined time interval with the first signal sampled for the same microtest well or vessel, whereby any change in optical density which is greater than about 0.05 units is said to indicate that aggregation or agglutination occurred in the well or other vessel. The present method is suitably applied to the measurement of aggregation in platelets.

Abstract translation: 用于同时测量多个微测井或其他样品保持容器中的聚集或凝集的快速方法包括提供多个光束，其具有适于聚焦在多个孔或其它容器的内容物上的离散焦点，以及 多个光电检测器，用于引导光束通过孔或其它容器及其内容物，以圆周运动连续地搅动孔或其它容器及其内容物，以有效地允许聚集或凝集，同时保持材料的完整性，产生多个 来自检测器的输出信号与光束在通过孔或其他容器及其内容物时的光吸收率成比例，以预定的时间间隔重复采样信号，并根据每个预定值之后获得的信号确定光密度的变化 通过比较每个信号的时间间隔 在每个预定时间间隔之后采样，对于相同的微测井或容器采样第一信号，由此大于约0.05单位的光密度的任何变化据说表明孔或其它容器中发生聚集或凝集。 本方法适用于血小板聚集的测定。

公开(公告)号：US5073029A

公开(公告)日：1991-12-17

申请号：US481450

申请日：1990-02-16

Applicant: James P. Eberly ,  Ralph A. Magnotti, Jr.

Inventor： James P. Eberly ,  Ralph A. Magnotti, Jr.

IPC: G01N21/27 ,  G01N21/25 ,  G01N21/77

CPC classification number: G01N21/253 ,  G01N21/255 ,  G01N2201/0407 ,  G01N2201/0446 ,  G01N2201/0621

Abstract: An optoelectronic device is provided which permits rapid sequential measurement of the optical density of multiple samples. The device employs a plurality of light-emitting diodes (LEDs) as light sources and a plurality of photodiodes as light detectors. In a preferred embodiment, arrays of LEDs paired with photodiodes are adapted to measure the optical density of samples contained in multiwell plastic plates (microplates) conventionally used for immunoassays.

Abstract translation: 提供了一种允许快速连续测量多个样品的光密度的光电子器件。 该装置使用多个发光二极管（LED）作为光源和多个光电二极管作为光检测器。 在优选实施例中，与光电二极管配对的LED阵列适于测量通常用于免疫测定的多孔塑料板（微孔板）中包含的样品的光密度。

公开(公告)号：US4892409A

公开(公告)日：1990-01-09

申请号：US218874

申请日：1988-07-14

Applicant: Harry F. Smith

Inventor： Harry F. Smith

IPC: G01N21/25

CPC classification number: G01N21/253 ,  G01N2201/0407 ,  G01N2201/0446 ,  G01N2201/0461 ,  G01N2201/065

Abstract: The invention relates to photometric apparatus and method for determining a characteristic of individual ones of a plurality of samples contained within a plurality of sample chambers. The photometric apparatus includes an enclosure; a substantially uniform source of radiation coupled to the enclosure such that a plurality of sample chambers, disposed within the enclosure, are simultaneously illuminated by the uniform source; and apparatus for detecting an amount of radiation which is transmitted through individual ones of the sample chambers, the detecting apparatus having an output signal having a magnitude which is a function of the amount of radiation which is transmitted through an individual one of the sample chambers. The substantially uniform source of radiation may include at least one source of radiation having an output comprising wavelengths within a first range of wavelengths and an optically integrating sphere having a radiation input port coupled to the output of the source of radiation and a radiation output port coupled to the enclosure. The photometric apparatus may further include radiation directing devices interposed between the radiation output port and the plurality of sample chambers for simultaneously directing radiation emanating from the output port into each of the sample chambers. The directed radiation may be either focussed or collimated.

Abstract translation: 本发明涉及用于确定包含在多个样品室内的多个样品中的各个样品的特性的光度测定装置和方法。 测光装置包括外壳; 耦合到外壳的基本上均匀的辐射源，使得设置在外壳内的多个样品室同时被均匀光源照射; 以及用于检测通过单个样本室传输的辐射量的装置，该检测装置具有输出信号，该输出信号的幅度是通过单独一个样本室传输的辐射量的函数。 基本上均匀的辐射源可以包括至少一个辐射源，其具有包括在第一波长范围内的波长的输出和具有耦合到辐射源的输出的辐射输入端口的光学积分球，以及辐射输出端口 到外壳。 测光装置还可以包括插入在辐射输出端口和多个样品室之间的辐射导向装置，用于同时将从输出端口发出的辐射引导到每个样品室中。 定向辐射可以是聚焦的或准直的。