公开(公告)号：US20150031080A1

公开(公告)日：2015-01-29

申请号：US14513087

申请日：2014-10-13

Applicant: NOVOZYMES, INC.

Inventor： Mark Wogulis

IPC: C12N9/42 ,  C12P19/14 ,  C12N15/82 ,  C12P19/02

CPC classification number: C12N9/2437 ,  C12N15/8257 ,  C12P19/02 ,  C12P19/14 ,  C12P2203/00 ,  C12Y302/01091 ,  Y02P20/52

Abstract: The present invention relates to variants of a parent cellobiohydrolase II. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

Abstract translation: 本发明涉及亲本纤维二糖水解酶II的变体。 本发明还涉及编码这些变体的多核苷酸; 核酸构建体，载体和包含所述多核苷酸的宿主细胞; 以及使用这些变体的方法。

公开(公告)号：US20150024446A1

公开(公告)日：2015-01-22

申请号：US14510582

申请日：2014-10-09

Applicant: Novozymes, Inc.

Inventor： Stephen Brown ,  Sheryl Luttringer ,  Debbie Yaver ,  Alan Berry

IPC: C12P7/46 ,  C12N15/80

CPC classification number: C12P7/46 ,  C07K14/38 ,  C12N15/80

Abstract: The present invention relates to methods of producing a C4 dicarboxylic acid, comprising: (a) cultivating a filamentous fungal host cell comprising a polynucleotide selected from the group consisting of a heterologous first polynucleotide encoding a C4 dicarboxylic acid transporter, a heterologous second polynucleotide encoding a malate dehydrogenase, and a heterologous third polynucleotide encoding a pyruvate carboxylase; wherein the filamentous fungal host cell is capable of secreting increased levels of the C4 dicarboxylic acid compared to the filamentous fungal host cell without the heterologous polynucleotide when cultivated under the same conditions; and (b) recovering the C4 dicarboxylic acid. The present invention also relates to methods for increasing C4 dicarboxylic acid production, filamentous fungal host cells and malate dehydrogenase variants.

Abstract translation: 本发明涉及生产C4二羧酸的方法，其包括：（a）培养丝状真菌宿主细胞，所述丝状真菌宿主细胞包含选自以下的多核苷酸：编码C4二羧酸转运蛋白的异源第一多核苷酸，编码 苹果酸脱氢酶和编码丙酮酸羧化酶的异源第三多核苷酸; 其中当在相同条件下培养时，丝状真菌宿主细胞能够分泌与不含异源多核苷酸的丝状真菌宿主细胞相比增加的C4二羧酸水平; 和（b）回收C4二羧酸。 本发明还涉及增加C4二羧酸生产，丝状真菌宿主细胞和苹果酸脱氢酶变体的方法。

公开(公告)号：US20150024439A1

公开(公告)日：2015-01-22

申请号：US14500520

申请日：2014-09-29

Applicant: Novozymes, Inc.

Inventor： Kimberly Brown ,  Paul Harris ,  Elizabeth Zaretsky ,  Edward Re ,  Elena Vlasenko ,  Keith McFarland ,  Alfredo Lopez de Leon

IPC: C12P7/14 ,  C11D3/386 ,  C12P19/02 ,  C12N9/42 ,  C12P19/14

CPC classification number: C07K14/37 ,  C11D3/38645 ,  C12N1/12 ,  C12N1/14 ,  C12N1/16 ,  C12N1/20 ,  C12N9/2437 ,  C12N9/2445 ,  C12N15/52 ,  C12N15/63 ,  C12P7/14 ,  C12P19/02 ,  C12P19/14 ,  C12P2201/00 ,  C12P2203/00 ,  C12Y302/01021 ,  Y02E50/16 ,  Y02E50/17

Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.

Abstract translation: 本发明涉及具有纤维素分解增强活性的分离的多肽和编码多肽的分离的多核苷酸。 本发明还涉及包含多核苷酸的核酸构建体，载体和宿主细胞以及生产和使用多肽的方法。

公开(公告)号：US20150007369A1

公开(公告)日：2015-01-01

申请号：US14358642

申请日：2012-11-21

Applicant: NOVOZYMES, INC. ,  NOVOZYMES A/S

Inventor： Janine Lin ,  Doreen Bohan ,  Michelle Maranta ,  Leslie Beresford ,  Michael Lamsa ,  Bjarne Gram Hansen ,  Frank Winther Rasmussen ,  Matt Sweeney ,  Douglas J. Boyle, III

IPC: C12P19/14 ,  C11D3/386 ,  C12P7/14 ,  C12N9/02 ,  C12P19/02

CPC classification number: C12N9/2402 ,  C11D3/386 ,  C12N9/0004 ,  C12N9/0071 ,  C12P19/02 ,  D21C5/005 ,  D21H17/005

Abstract: The present invention relates to GH61 polypeptide variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

Abstract translation: 本发明涉及GH61多肽变体。 本发明还涉及编码这些变体的多核苷酸; 核酸构建体，载体和包含所述多核苷酸的宿主细胞; 以及使用这些变体的方法。

公开(公告)号：US20150004656A1

公开(公告)日：2015-01-01

申请号：US14486729

申请日：2014-09-15

Applicant: Novozymes A/S ,  Novozymes, Inc.

Inventor： Lan Tang ,  Ye Liu ,  Junxin Duan ,  Yu Zhang ,  Christian Joergensen ,  Randall Kramer

IPC: C12N9/42 ,  C12P19/02 ,  C12P19/14 ,  C12N15/01 ,  C12N15/82 ,  C12N15/113

CPC classification number: C12N9/2437 ,  C07K14/385 ,  C12N9/2434 ,  C12N15/01 ,  C12N15/1137 ,  C12N15/8218 ,  C12N15/8246 ,  C12N2310/14 ,  C12N2310/141 ,  C12P7/14 ,  C12P19/02 ,  C12P19/14 ,  C12P2203/00

Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

公开(公告)号：US20140329293A1

公开(公告)日：2014-11-06

申请号：US13765166

申请日：2013-02-12

Applicant: NOVOZYMES A/S ,  NOVOZYMES INC.

Inventor： Nikolaj Spodsberg

IPC: C12N9/24

CPC classification number: C12N9/2437 ,  C12N1/00 ,  C12N1/14 ,  C12N1/20 ,  C12P5/00 ,  C12P7/00 ,  C12P7/02 ,  C12P7/06 ,  C12P13/04 ,  C12P19/02 ,  C12P19/14 ,  C12P2203/00 ,  Y02P20/52

Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Abstract translation: 本发明涉及具有纤维二糖水解酶活性的分离的多肽和编码该多肽的多核苷酸。 本发明还涉及包含多核苷酸的核酸构建体，载体和宿主细胞以及产生和使用多肽的方法。

公开(公告)号：US08865445B2

公开(公告)日：2014-10-21

申请号：US14052176

申请日：2013-10-11

Applicant: Novozymes, Inc.

Inventor： Suchindra Maiyuran ,  Randall Kramer ,  Paul Harris

IPC: A01H5/00 ,  A01H1/00 ,  A01N63/00 ,  A23K3/00 ,  C12N9/24 ,  C12P21/00 ,  C07H21/04 ,  C11D3/386 ,  C12N9/42 ,  C12P19/14

CPC classification number: C12N9/2437 ,  C11D3/386 ,  C11D3/38645 ,  C11D3/3869 ,  C12P19/02 ,  C12P19/14 ,  C12Y302/01 ,  Y02E50/16

Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

公开(公告)号：US08859253B2

公开(公告)日：2014-10-14

申请号：US14034209

申请日：2013-09-23

Applicant: Novozymes, Inc.

Inventor： Mark Wogulis

IPC: A01H5/00 ,  A01H1/00 ,  A01N63/00 ,  A23K3/00 ,  C12N9/24 ,  C12P21/00 ,  C07H21/04 ,  C12N9/42 ,  C12P19/14

CPC classification number: C12N9/2437 ,  C12N15/8257 ,  C12P19/02 ,  C12P19/14 ,  C12P2203/00 ,  C12Y302/01091 ,  Y02P20/52

Abstract: The present invention relates to variants of a parent cellobiohydrolase II. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

公开(公告)号：US20140304859A1

公开(公告)日：2014-10-09

申请号：US14359976

申请日：2012-12-14

Applicant: Novozymes Inc.

Inventor： Ye Liu ,  Yu Zhang ,  Junxin Duan ,  Lan Tang

IPC: C12N9/42 ,  C12P19/02 ,  C12P7/04 ,  C12P7/16 ,  C12P7/14 ,  C12P7/20 ,  C12P7/18 ,  C12P5/02 ,  C12P5/00 ,  C12P3/00 ,  C12P13/20 ,  C12P13/14 ,  C12P13/04 ,  C12P13/08 ,  C12P13/06 ,  C12P7/54 ,  C12P7/40 ,  C12P7/46 ,  C12P7/60 ,  C12P7/42 ,  C12P7/56 ,  C12P7/52 ,  C12P7/58 ,  C12P19/14

CPC classification number: C12N9/244 ,  C12N9/2437 ,  C12N15/1137 ,  C12N2310/14 ,  C12P3/00 ,  C12P5/002 ,  C12P5/02 ,  C12P7/04 ,  C12P7/14 ,  C12P7/16 ,  C12P7/18 ,  C12P7/20 ,  C12P7/40 ,  C12P7/42 ,  C12P7/46 ,  C12P7/52 ,  C12P7/54 ,  C12P7/56 ,  C12P7/58 ,  C12P7/60 ,  C12P13/04 ,  C12P13/06 ,  C12P13/08 ,  C12P13/14 ,  C12P13/20 ,  C12P19/02 ,  C12P19/14 ,  C12P2203/00 ,  C12Y302/01004

Abstract: Provided are isolated polypeptides having endoglucanase activity and polynucleotides encoding the polypeptides. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Abstract translation: 提供具有内切葡聚糖酶活性的分离的多肽和编码多肽的多核苷酸。 还提供了包含多核苷酸的核酸构建体，载体和宿主细胞以及产生和使用多肽的方法。

公开(公告)号：US20140273119A1

公开(公告)日：2014-09-18

申请号：US14357611

申请日：2012-11-30

Applicant: Novozymes Inc.

Inventor： Ye Liu ,  Lan Tang ,  Yu Zhang ,  Junxin Duan

IPC: C12P19/14 ,  C12P19/02 ,  C12P7/26 ,  C12P7/40 ,  C12P7/04 ,  C12N15/113 ,  C12P7/14

CPC classification number: C12N9/2482 ,  C12N9/2434 ,  C12P7/10 ,  C12P19/02 ,  C12P19/14 ,  C12P2203/00 ,  C12Y302/01008 ,  C12Y302/01037 ,  D21C5/005

Abstract: Provided are isolated polypeptides having beta-xylosidase activity and polynucleotides encoding the polypeptides. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Abstract translation: 提供具有β-木糖苷酶活性的分离的多肽和编码多肽的多核苷酸。 还提供了包含多核苷酸的核酸构建体，载体和宿主细胞以及产生和使用多肽的方法。