公开(公告)号：US20060205013A1

公开(公告)日：2006-09-14

申请号：US11336110

申请日：2006-01-20

Applicant: Jeo-young Shim ,  Su-hyeon Kim ,  Kyu-tae Yoo ,  Sung-ouk Jung ,  Joon-shik Park

Inventor： Jeo-young Shim ,  Su-hyeon Kim ,  Kyu-tae Yoo ,  Sung-ouk Jung ,  Joon-shik Park

IPC: G01N33/53

CPC classification number: B82Y30/00 ,  B82Y15/00 ,  G01N27/4145

Abstract: Provided is a field effect transistor (FET) type biosensor including a source electrode, a gate, and a drain electrode. A ligand that can bind to a side of a nucleic acid is added to the surface of the gate. In a conventional FET type biosensor, it is difficult to detect a signal within the debye length because a target nucleic acid is directly fixed to the surface of a gate of the conventional FET. However, in the present invention, this problem can be overcome and the debye length can be increased by treating the surface of a gate of an FET sensor with a ligand that can bind to a side of a nucleic acid. The ligand can be adsorbed onto the surface of the gate. In this case, the nucleic acid is adsorbed parallel to the surface of the gate, not perpendicular to the surface of the gate, thus generating an effective depletion region. In addition, hybridization efficiency can be increased because a hybridized sample can be injected into an FET sensor at high ionic strength.

Abstract translation: 提供了包括源电极，栅极和漏电极的场效应晶体管（FET）型生物传感器。 可以结合核酸一侧的配体加入到门的表面。 在传统的FET型生物传感器中，由于目标核酸直接固定在常规FET的栅极表面，难以检测德拜长度内的信号。 然而，在本发明中，可以克服这个问题，并且可以通过用可以结合核酸一侧的配体处理FET传感器的栅极的表面来增加德拜长度。 配体可以吸附在栅极的表面上。 在这种情况下，核酸被平行于栅极的表面吸附，而不垂直于栅极的表面，从而产生有效的耗尽区域。 此外，杂交效率可以提高，因为杂化样品可以以高离子强度注入FET传感器。

公开(公告)号：US20060188907A1

公开(公告)日：2006-08-24

申请号：US11336294

申请日：2006-01-18

Applicant: In-ho Lee ,  Jun-hong Min ,  Su-hyeon Kim ,  Kui-hyun Kim ,  Seung-yeon Yang

Inventor： In-ho Lee ,  Jun-hong Min ,  Su-hyeon Kim ,  Kui-hyun Kim ,  Seung-yeon Yang

IPC: C12Q1/68 ,  C12M1/34 ,  H01L21/00

CPC classification number: C23C18/1893 ,  B01J19/0046 ,  B01J2219/00527 ,  B01J2219/00596 ,  B01J2219/00603 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00743 ,  C23C18/1608 ,  C23C18/2086 ,  C23C18/44 ,  C40B40/06 ,  C40B40/10 ,  G01N33/54353 ,  H05K3/389 ,  H05K2203/013

Abstract: Provided is a linker functional group patterning method for biomolecule immobilization. The patterning method includes preparing a coating composition including a hydrophobic group-containing silane compound and a hydrophilic group-containing silane compound; forming a surface tension control layer by coating the coating composition on a substrate for biomoleucle immobilization; and forming a linker functional group pattern on the surface tension control layer using a coating composition including a linker functional group-containing compound followed by thermal treatment. The linker functional group pattern is formed in a uniform size and distribution and contains high-density linker functional groups.

Abstract translation: 提供了用于生物分子固定的接头官能团图案化方法。 图案化方法包括制备包含含疏水基的硅烷化合物和含亲水基团的硅烷化合物的涂料组合物; 通过将所述涂料组合物涂布在用于生物核固定的底物上来形成表面张力控制层; 以及使用包含连接官能团的化合物的涂层组合物在表面张力控制层上形成接头官能团图案，随后进行热处理。 连接体官能团图案形成均匀的大小和分布，并含有高密度接头官能团。

公开(公告)号：US07090977B2

公开(公告)日：2006-08-15

申请号：US10282251

申请日：2002-10-29

Applicant: Kyu-Sik Yun ,  Jeong-Gun Lee ,  Je-Kyun Park ,  Su-Hyeon Kim ,  Sang-Eun Lee

Inventor： Kyu-Sik Yun ,  Jeong-Gun Lee ,  Je-Kyun Park ,  Su-Hyeon Kim ,  Sang-Eun Lee

IPC: C12Q1/68

CPC classification number: C12Q1/6816 ,  Y10T436/143333 ,  C12Q2563/173

Abstract: Disclosed is a method for detecting nucleic acid hybridization by using intercalator binding to hybridized nucleic acid, wherein oxidation-reduction of transition metallic complex is induced to cause electrochemiluminescence, thereby providing a method for detecting nucleic acid hybridization without a special labeling.

Abstract translation: 公开了通过使用嵌入剂与杂交核酸结合来检测核酸杂交的方法，其中诱导过渡金属络合物的氧化还原引起电化学发光，从而提供用于检测核酸杂交而不进行特殊标记的方法。

公开(公告)号：US20060118417A1

公开(公告)日：2006-06-08

申请号：US11281226

申请日：2005-11-16

Applicant: Young-a Kim ,  Jun-hong Min ,  Kui-hyun Kim ,  Myo-yong Lee ,  Su-hyeon Kim ,  Young-nam Kwon ,  Jeong-gun Lee ,  Joon-ho Kim

Inventor： Young-a Kim ,  Jun-hong Min ,  Kui-hyun Kim ,  Myo-yong Lee ,  Su-hyeon Kim ,  Young-nam Kwon ,  Jeong-gun Lee ,  Joon-ho Kim

IPC: C07H21/04

CPC classification number: C07H21/04

Abstract: Provided is a method of purifying nucleic acids using hydrogen bonding and an electric field, including: bringing a sample containing target nucleic acids into contact with an electrode coated with a material capable of forming hydrogen bonds with the target nucleic acids; applying a positive voltage to the electrode to move the target nucleic acids closer to the electrode so as to form hydrogen bonds with the material on the electrode; washing the electrode; and applying to the electrode a negative voltage to elute the bound target nucleic acids. According to the method, selectivity to nucleic acids and proteins increases due to hydrogen bonding, nucleic acid purification is possible within a short time through an electric field, and the bound nucleic acids can be efficiently eluted.

Abstract translation: 提供了使用氢键和电场来净化核酸的方法，包括：使含有靶核酸的样品与涂覆有能够与靶核酸形成氢键的材料接触; 向电极施加正电压以使靶核酸更靠近电极以与电极上的材料形成氢键; 清洗电极; 并向电极施加负电压以洗脱结合的靶核酸。 根据该方法，由于氢键，核酸和蛋白质的选择性增加，核酸纯化可以在短时间内通过电场进行，并且结合的核酸可以被有效地洗脱。

公开(公告)号：US20060110760A1

公开(公告)日：2006-05-25

申请号：US11269037

申请日：2005-11-07

Applicant: Su-hyeon Kim ,  In-ho Lee ,  Jun-hong Min

Inventor： Su-hyeon Kim ,  In-ho Lee ,  Jun-hong Min

IPC: C12Q1/68 ,  C12M1/34

CPC classification number: B82Y30/00 ,  B01J19/0046 ,  B01J2219/00369 ,  B01J2219/00432 ,  B01J2219/005 ,  B01J2219/0052 ,  B01J2219/00641 ,  B01J2219/00644 ,  B01J2219/00657 ,  B01J2219/00673 ,  B01J2219/00677 ,  B01J2219/00711 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00729 ,  B01J2219/00743

Abstract: A microarray including hydrogel and a plurality of probes which are immobilized in discrete regions of the hydrogel, and a method of preparing the same are provided. When using the microarray and method, a solid substrate is not required and many biomolecules can be immobilized in a small volume, thereby obtaining high sensitivity. Since gel can be cut to obtain many pieces, many microarrays can be prepared at once.

Abstract translation: 提供了包含水凝胶和固定在水凝胶的离散区域中的多个探针的微阵列及其制备方法。 当使用微阵列和方法时，不需要固体底物，并且许多生物分子可以以小体积固定，从而获得高灵敏度。 由于可以切割凝胶以获得许多片，因此可以一次制备许多微阵列。

公开(公告)号：US20050140978A1

公开(公告)日：2005-06-30

申请号：US10933084

申请日：2004-09-02

Applicant: Su-hyeon Kim ,  Jin-tae Kim

Inventor： Su-hyeon Kim ,  Jin-tae Kim

IPC: C12M1/00 ,  C12M1/34 ,  G01N21/03 ,  G01N21/64 ,  G01N21/78 ,  G01N21/88

CPC classification number: G01N21/645 ,  G01N2021/6482

Abstract: An ultra small fluorescence detector capable of detecting in real time reaction undergoing in a micro chamber having a predetermined volume and disposed on a microfluid chip is provided. The fluorescence detector for detecting in real time PCR amplification undergoing in the microfluid chip having a micro chamber with a predetermined volume includes a light source generating an excitation beam, a first optical system capable of irradiating the excitation beam having a predetermined spot size to the micro chamber, a first detector, and a second optical system reflecting a fluorescent beam derived from the excitation beam having the predetermined spot size in the micro chamber to the first detector. Accordingly, the fluorescence detector is designed such that light emitted by a light source is focused between a first mirror and an objective lens. Therefore, the spot size of an excitation beam transmitted by the objective lens is largely formed so that the excitation beam can be irradiated on the whole micro chamber of the microfluid chip, thereby detecting a fluorescent beam on a broader area.

Abstract translation: 提供了能够实时检测具有预定体积的微室中并设置在微流体芯片上的超小荧光检测器。 用于实时检测在具有预定体积的微室的微流体芯片中进行PCR扩增的荧光检测器包括产生激发光束的光源，能够将具有预定光斑尺寸的激发光束照射到微细的第一光学系统 第一检测器和第二光学系统，其将来自具有在微室中的预定光斑尺寸的激发光束的荧光束反射到第一检测器。 因此，荧光检测器被设计成使得由光源发射的光聚焦在第一反射镜和物镜之间。 因此，由物镜透射的激发光束的光斑尺寸大大地形成为使得激发光束可以照射在微流体芯片的整个微室上，从而在更广泛的区域上检测荧光束。