公开(公告)号：US12202857B2

公开(公告)日：2025-01-21

申请号：US17314541

申请日：2021-05-07

Applicant: Waters Technologies Corporation

Inventor： Wenjing Li ,  Beatrice Muriithi ,  Anna Boardman ,  Matthew A. Lauber

IPC: C07K1/12 ,  G01N33/68

Abstract: The present disclosure relates to a method of digesting a sample comprising protein, the method comprising: adding the sample to an apparatus containing a buffer and a solid support surface comprising a surface coating, wherein the surface coating immobilizes enzyme while reducing undesired interactions between the sample and the solid support surface; immobilizing enzymes on the surface coating for digestion of the protein of the sample; and heating the sample to complete a heat-assisted digestion of the protein.

公开(公告)号：US12181452B2

公开(公告)日：2024-12-31

申请号：US17204021

申请日：2021-03-17

Applicant: Waters Technologies Corporation

Inventor： Stephen J. Shiner ,  Cheryl Boissel ,  Matthew A. Lauber ,  Kevin Wyndham ,  Michael O. Fogwill

IPC: G01N30/60 ,  B01D15/22 ,  B05D1/00 ,  B05D7/14 ,  G01N30/02

Abstract: A chromatographic device including a coated metallic frit is disclosed. The coating is provided over the fluid exposed surfaces of the frit, thereby covering metallic surfaces to prevent interaction with an analyte in the flowstream. This technology relates to the use of a vapor deposition coated frit together with an uncoated frit in a liquid flow path for improved chromatography. More specifically, this technology relates to liquid chromatographic devices for separating analytes in a sample having a single coated frit within an uncoated metallic fluidic flow path (i.e., the column tube or channel is formed of stainless steel, titanium (pure or alloyed), or some mixture of stainless steel and titanium) and does not include the coating applied to the frit.

公开(公告)号：US20240416259A1

公开(公告)日：2024-12-19

申请号：US18742319

申请日：2024-06-13

Applicant: Waters Technologies Corporation

Inventor： Lavelay Kizekai ,  Michael Dion ,  Stephen Shiner ,  Balasubrahmanyam Addepalli ,  Matthew A. Lauber

IPC: B01D15/34 ,  G01N21/47

Abstract: The present technology provides a description of a flow column dampener device capable of decreasing the MALS noise from solvent contaminants and transient ripples induced by the HPLC pump prior to the evaluation and characterization of the biophysical properties of macromolecules. This dampener typically includes a particle bed column that can trap and remove the noise-inducing soluble particulates from mobile phase, while simultaneously dampening the noise induced by pump-induced ripples. The types of sorbents, sorbent particle size range and column size formats of the device provides significant reduction in MALS noise prior to the evaluation. This device enables confident characterization of cell and gene therapy products with accurate measurements of the physical properties of the macromolecule analytes such as cell and gene therapy products.

公开(公告)号：US11998862B2

公开(公告)日：2024-06-04

申请号：US18179835

申请日：2023-03-07

Applicant: Waters Technologies Corporation

Inventor： Raymond P. Fisk ,  Timothy J. Becker ,  Mary Elizabeth Lame ,  Frank John Marszalkowski ,  Matthew A. Lauber

IPC: B01L3/00 ,  B01D15/12 ,  B01D15/14 ,  B01D15/22 ,  B01D15/38 ,  B01D15/42 ,  B01L3/02

CPC classification number: B01D15/3809 ,  B01D15/12 ,  B01D15/14 ,  B01D15/22 ,  B01D15/424 ,  B01L3/5023 ,  B01L3/0275 ,  B01L3/5021 ,  B01L2200/0631 ,  B01L2200/0668 ,  B01L2300/0681 ,  B01L2300/069 ,  B01L2400/0409 ,  B01L2400/0433

Abstract: The present disclosure pertains to sample preparation devices useful for affinity capture and purification that include one or more internal structures that comprise a reservoir, a well, a fluid passageway, sorbent particles, and a filter element that blocks passage of the affinity sorbent particles, which sample preparation devices combine the attributes of both dispersive and flow through designs into a single sample preparation device. The present disclosure also pertains to kits that contain and methods that use such sample preparation devices.

公开(公告)号：US20240084279A1

公开(公告)日：2024-03-14

申请号：US18366035

申请日：2023-08-07

Applicant: Waters Technologies Corporation

Inventor： Balasubrahmanyam Addepalli ,  Abraham S. Finny ,  Matthew A. Lauber

IPC: C12N9/50

CPC classification number: C12N9/50 ,  C12Y304/21004 ,  C12Y304/2105 ,  C12Y304/24033

Abstract: This present disclosure relates to engineered protease enzymes, including trypsin, Lys-C and Asp-N proteases, that have enhanced autolysis resistance. Also disclosed herein are methods of using such engineered enzymes for improving detection of target analyte proteins in an analytical assay.

公开(公告)号：US11572563B2

公开(公告)日：2023-02-07

申请号：US16913805

申请日：2020-06-26

Applicant: Waters Technologies Corporation

Inventor： Xiaoxiao Liu ,  Matthew A. Lauber

IPC: C12N15/115 ,  C07K16/28 ,  G01N33/561 ,  G01N33/68

Abstract: A method of capturing human immunoglobulin Fc domains in a biofluid sample is provided. The method includes providing an affinity capture device. The affinity capture device includes a surface having an aptamer that is at least 80% identical to SEQ ID NO 1 immobilized onto the surface of the affinity capture device. The biofluid sample is diluted with a binding buffer. The binding buffer includes (A) tris(hydroxymethyl)aminomethane (Tris), trimethylamine (TES), 2-ethanesulfonic acid (MES), or 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES); (B) a magnesium cation at a concentration between about 10 μM to about 20 mM; and (C) a total monovalent cation concentration from 0 to no greater than 100 mM. The human immunoglobulin Fc domains in the biofluid sample are adsorbed to the aptamer with the binding buffer.

公开(公告)号：US20220381788A1

公开(公告)日：2022-12-01

申请号：US17734690

申请日：2022-05-02

Applicant: Waters Technologies Corporation

Inventor： Darryl W. Brousmiche ,  Matthew A. Lauber

IPC: G01N33/58 ,  G01N33/50

Abstract: Methods for derivatization of biomolecules including glycans or other biopolymers with one or more fluorescent, MS active compounds by reductive amination or rapid tagging in order to produce derivatized glycan having a pKa >7 and between about 200 Å and about 1000 Å of nonpolar surface area are described.

公开(公告)号：US20220275022A1

公开(公告)日：2022-09-01

申请号：US17592260

申请日：2022-02-03

Applicant: Waters Technologies Corporation

Inventor： Matthew A. Lauber ,  Xiaoxiao Liu

IPC: C07K1/22 ,  B01J20/32 ,  B01J20/286 ,  C07K1/16

Abstract: In some aspects, the present disclosure pertains to sample treatment methods that comprise: contacting an acidic elution solution that is free of primary amine, secondary amine and thiol groups with a sorbent having bound target antibody and separating the elution solution from the sorbent, thereby releasing bound target antibody from the sorbent and forming a first collection fraction that comprises the elution solution and released target antibody; contacting the sorbent with a neutralization buffer solution that is free of primary amine, secondary amine and thiol groups and separating the neutralization buffer solution from the sorbent, thereby forming a second collection fraction that comprises the neutralization buffer solution; and forming a neutralized solution that comprises the first collection fraction and the second collection fraction. In other aspects, the present disclosure pertains to kits for performing such sample treatment methods.

公开(公告)号：US11414785B2

公开(公告)日：2022-08-16

申请号：US16990568

申请日：2020-08-11

Applicant: Waters Technologies Corporation

Inventor： Matthew A. Lauber ,  Xiaoxiao Liu ,  Beatrice Muriithi ,  Edouard S. P. Bouvier

IPC: C40B40/10 ,  C12N15/10

Abstract: The present disclosure relates to protein arrays and methods of using the same for the detection, quantification and characterization of biomolecules that specifically bind to the array among various other biomolecules in a biological sample. Specifically, the present disclosure relates to protein arrays that include a plurality of immunoglobulin molecules derived from shark single-domain heavy chain antibody lacking light-chains but including at least one variable antigen-binding domain with a binding site for an antigen. The immunoglobulin molecules are immobilized on a substrate via a linker. Further encompassed herein are diagnostic devices and kits, comprising the protein array and methods of using same.

公开(公告)号：US11371996B2

公开(公告)日：2022-06-28

申请号：US15522908

申请日：2015-10-28

Applicant: Waters Technologies Corporation

Inventor： Matthew A. Lauber ,  Darryl W. Brousmiche ,  Stephan M. Koza

IPC: C12P19/28 ,  G01N33/58 ,  G01N33/68

Abstract: Methods of analyzing glycosylated biomolecules include the steps of producing a deglycosylation mixture of biomolecules deglycosylated by natural or synthetic enzymatic or chemical techniques; providing a reagent solution having a labeling reagent in a polar aprotic, non-nucleophilic organic solvent; and mixing the deglycosylation mixture with the reagent solution in an excess of labeling reagent to produce derivatized glycosylamines. The method steps can be carried out purposefully without depletion of protein matter. A quenching solution can be added to the reaction mixture so that the pH of the reaction mixture is shifted to above 10. The yield of derivatized glycosylamines can be in an amount of about 80 to about 100 mole percent of the reaction mixture with minimal overlabeling, less than 0.2 mole percent. The derivizated glycosylamines can be separated from the reaction mixture and detected by chromatographic detection, fluorescence detection, mass spectrometry (“MS”), or Ultra Violet (“UV”) detection and/or a combination thereof.