公开(公告)号：US09528132B2

公开(公告)日：2016-12-27

申请号：US14405504

申请日：2013-06-03

Applicant: CARBIOS

Inventor： Roberto Mazzoli ,  Enrica Pessione ,  Loredana Tarraran ,  Chiara Gandini

IPC: C12N9/18 ,  C12P7/56 ,  C12N9/24 ,  C12N15/00 ,  C07H21/04 ,  C12N9/42

CPC classification number: C12P7/56 ,  C07K2319/02 ,  C07K2319/20 ,  C07K2319/70 ,  C12N9/18 ,  C12N9/2437 ,  C12N9/2445 ,  C12P2203/00 ,  C12Y301/01073

Abstract: The present invention relates to a polynucleotide encoding a recombinant scaffolding polypeptide comprising at least a signal peptide, a Cellulose Binding Domain, two cohesin domains and an S-layer Homology domain, wherein said isolated polynucleotide preferably comprises all or an active part of the nucleotide sequence as set forth in SEQ ID NO:2. The present invention further relates to vectors comprising such polynucleotides, recombinant lactic acid bacteria, and method for degrading a cellulosic biomass using such recombinant lactic acid bacteria.

Abstract translation: 本发明涉及编码重组支架多肽的多核苷酸，其包含至少信号肽，纤维素结合结构域，两个内含蛋白结构域和S层同源结构域，其中所述分离的多核苷酸优选包含核苷酸序列的全部或活性部分 如SEQ ID NO：2所示。 本发明还涉及包含这种多核苷酸的载体，重组乳酸菌和使用这种重组乳酸菌降解纤维素生物质的方法。

公开(公告)号：US20160280881A1

公开(公告)日：2016-09-29

申请号：US15034655

申请日：2014-11-04

Applicant: CARBIOS

Inventor： CÉDRIC BOISART ,  EMMANUEL MAILLE ,  FRÉDÉRIQUE GUILLAMOT

IPC: C08J11/10 ,  B09B3/00 ,  C12P7/56

CPC classification number: C08J11/105 ,  B09B3/00 ,  C08J2323/08 ,  C08J2329/04 ,  C08J2367/02 ,  C08J2367/04 ,  C08J2377/06 ,  C12P7/56 ,  Y02W30/702

Abstract: The present invention relates to a method for degrading a plastic containing non-biodegradable polymers comprising submitting said plastic to at least one enzyme for modifying a polymer of said plastic which has a methane potential less than 5 Nm3/t+/−20%, wherein at least one product resulting from the modification exhibits a methane potential greater than 10 Nm3/t+/−20%.

Abstract translation: 本发明涉及一种降解包含不可生物降解聚合物的塑料的方法，包括将所述塑料递送至至少一种酶，用于改变甲烷电位小于5Nm 3 / t +/- 20％的所述塑料的聚合物，其中在 由改性产生的至少一种产物的甲烷电势大于10Nm 3 / t +/- 20％。

公开(公告)号：US20150056673A1

公开(公告)日：2015-02-26

申请号：US14387285

申请日：2013-03-27

Applicant: CARBIOS

Inventor： Cédric Boisart

IPC: C12P7/62 ,  C12P7/56

CPC classification number: C12P7/625 ,  C12N9/54 ,  C12P7/56 ,  C12P39/00 ,  Y02P20/52

Abstract: The invention concerns a microorganism which is genetically modified so as to i) synthesize a hydrocarbon monomer by fermentation of a carbon source, and ii) depolymerize a polymer constituted at least by hydrocarbon monomer which it is capable of synthesizing. The invention also concerns a method for producing a hydrocarbon monomer using a genetically modified microorganism of this type, as well as the coculture of this microorganism with another microorganism which is capable of synthesizing a polymer of interest.

Abstract translation: 本发明涉及经遗传修饰的微生物，以便i）通过碳源的发酵来合成烃单体，和ii）至少由能够合成的烃单体构成的聚合物解聚。 本发明还涉及使用这种类型的遗传修饰的微生物来生产烃单体的方法，以及该微生物与能够合成感兴趣的聚合物的另一种微生物的共培养。