公开(公告)号：US20150167030A1

公开(公告)日：2015-06-18

申请号：US14405504

申请日：2013-06-03

Applicant: CARBIOS

Inventor： Roberto Mazzoli ,  Enrica Pessione ,  Loredana Tarraran ,  Chiara Gandini

IPC: C12P7/56 ,  C12N9/18

CPC classification number: C12P7/56 ,  C07K2319/02 ,  C07K2319/20 ,  C07K2319/70 ,  C12N9/18 ,  C12N9/2437 ,  C12N9/2445 ,  C12P2203/00 ,  C12Y301/01073

Abstract: The present invention relates to a polynucleotide encoding a recombinant scaffolding polypeptide comprising at least a signal peptide, a Cellulose Binding Domain, two cohesin domains and an S-layer Homology domain, wherein said isolated polynucleotide preferably comprises all or an active part of the nucleotide sequence as set forth in SEQ ID NO:2. The present invention further relates to vectors comprising such polynucleotides, recombinant lactic acid bacteria, and method for degrading a cellulosic biomass using such recombinant lactic acid bacteria.

Abstract translation: 本发明涉及编码重组支架多肽的多核苷酸，其包含至少信号肽，纤维素结合结构域，两个内含蛋白结构域和S层同源结构域，其中所述分离的多核苷酸优选包含核苷酸序列的全部或活性部分 如SEQ ID NO：2所示。 本发明还涉及包含这种多核苷酸的载体，重组乳酸菌和使用这种重组乳酸菌降解纤维素生物质的方法。

公开(公告)号：US09528132B2

公开(公告)日：2016-12-27

申请号：US14405504

申请日：2013-06-03

Applicant: CARBIOS

Inventor： Roberto Mazzoli ,  Enrica Pessione ,  Loredana Tarraran ,  Chiara Gandini

IPC: C12N9/18 ,  C12P7/56 ,  C12N9/24 ,  C12N15/00 ,  C07H21/04 ,  C12N9/42

CPC classification number: C12P7/56 ,  C07K2319/02 ,  C07K2319/20 ,  C07K2319/70 ,  C12N9/18 ,  C12N9/2437 ,  C12N9/2445 ,  C12P2203/00 ,  C12Y301/01073

Abstract: The present invention relates to a polynucleotide encoding a recombinant scaffolding polypeptide comprising at least a signal peptide, a Cellulose Binding Domain, two cohesin domains and an S-layer Homology domain, wherein said isolated polynucleotide preferably comprises all or an active part of the nucleotide sequence as set forth in SEQ ID NO:2. The present invention further relates to vectors comprising such polynucleotides, recombinant lactic acid bacteria, and method for degrading a cellulosic biomass using such recombinant lactic acid bacteria.

Abstract translation: 本发明涉及编码重组支架多肽的多核苷酸，其包含至少信号肽，纤维素结合结构域，两个内含蛋白结构域和S层同源结构域，其中所述分离的多核苷酸优选包含核苷酸序列的全部或活性部分 如SEQ ID NO：2所示。 本发明还涉及包含这种多核苷酸的载体，重组乳酸菌和使用这种重组乳酸菌降解纤维素生物质的方法。