公开(公告)号：US11680905B2

公开(公告)日：2023-06-20

申请号：US17572926

申请日：2022-01-11

Applicant: LIFE TECHNOLOGIES HOLDINGS PTE LIMITED ,  LIFE TECHNOLOGIES CORPORATION

Inventor： Ming Song Chen ,  Chee Woei Chong ,  Wuh Ken Loh ,  Wern Yuh Foo ,  Kuan Moon Boo ,  Norbert Leclerc ,  Kristina Uhlendorf ,  Reiner Koellner ,  Torsten Fuchs ,  Stefan Breitfelder

IPC: G01N21/64

CPC classification number: G01N21/6452 ,  G01N21/6428 ,  G01N21/6458 ,  G01N2021/6478

Abstract: A biological analysis system can include an excitation module and an emission module. The excitation module can include a collimator element for receiving excitation light from the excitation light source and transmitting collimated excitation light in a first direction, and a plurality of excitation mirrors arrayed along the excitation light path, each excitation mirror disposed at an acute angle relative to the first direction and configured to reflect collimated excitation light along a second direction. The emission module can be positioned to receive excitation light transmitted along the second direction and can include a sample block comprising a plurality of sample receptacles positioned to receive a beam of collimated excitation light, and a plurality of photodetectors configured to receive emission light transmitted from a respective sample receptacle in a direction transverse to the second direction of the excitation light path.

公开(公告)号：US20230175046A1

公开(公告)日：2023-06-08

申请号：US18104599

申请日：2023-02-01

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： David LIGHT ,  Wolfgang HINZ ,  Ronald CICERO ,  Christina INMAN ,  Paul KENNEY ,  Alexander MASTROIANNI ,  Roman ROZHKOV ,  Yufang WANG ,  Jeremy GRAY ,  Marc GLAZER ,  Dmitriy GREMYACHINSKIY

IPC: C12Q1/6837 ,  C12Q1/6869 ,  C12Q1/6834 ,  C12Q1/6806

CPC classification number: C12Q1/6837 ,  C12Q1/6869 ,  C12Q1/6834 ,  C12Q1/6806

Abstract: A method of forming a polymer matrix array includes applying an aqueous solution into wells of a well array. The aqueous solution includes polymer precursors. The method further includes applying an immiscible fluid over the well array to isolate the aqueous solution within the wells of the well array and polymerizing the polymer precursors isolated in the wells of the well array to form the polymer matrix array. An apparatus includes a sensor array, a well array corresponding to the sensor array, and an array of polymer matrices disposed in the well array.

公开(公告)号：US20230167210A1

公开(公告)日：2023-06-01

申请号：US18101785

申请日：2023-01-26

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Geir FONNUM ,  Grete Irene MODAHL ,  Nini Hofsloekken KJUS ,  Astrid Evenroed MOLTEBERG ,  Diem Thuy Thi TRAN ,  Jo AASERUD ,  M. Talha GOKMEN ,  Steven M. MENCHEN ,  Carl FULLER ,  Luisa ANDRUZZI ,  Wolfgang HINZ

IPC: C08F130/08 ,  C07F7/18 ,  C08F8/12 ,  C12Q1/686 ,  C08F2/20 ,  C08F2/26 ,  C08F230/08 ,  C08F6/24 ,  C08F222/38 ,  C08F8/32 ,  C08F8/34 ,  C12P19/34 ,  C12Q1/6874 ,  G01N27/414 ,  C08J9/20

CPC classification number: C08F130/08 ,  C07F7/1804 ,  C08F8/12 ,  C12Q1/686 ,  C07F7/188 ,  C08F2/20 ,  C08F2/26 ,  C08F230/085 ,  C08F6/24 ,  C08F222/385 ,  C08F8/32 ,  C08F8/34 ,  C12P19/34 ,  C12Q1/6874 ,  G01N27/4145 ,  C08J9/20 ,  Y10T428/2982 ,  C08F2800/20 ,  C08F220/603

Abstract: A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of mer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.

公开(公告)号：US11654408B2

公开(公告)日：2023-05-23

申请号：US16421168

申请日：2019-05-23

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Trevor G. Williams

IPC: B01F27/2111 ,  B01F35/513 ,  B01F35/41 ,  C12M1/02 ,  C12M1/00 ,  C12M1/10 ,  B01F27/07 ,  B01F27/2121 ,  B01F101/44

CPC classification number: B01F35/4111 ,  B01F27/071 ,  B01F27/2111 ,  B01F27/2121 ,  B01F35/513 ,  C12M1/007 ,  C12M1/02 ,  C12M1/10 ,  B01F2101/44

Abstract: A method for operating a bioreactor includes passing a drive shaft into a first tubular connector and a second tubular connector projecting from the first tubular connector, the first tubular connector and the second tubular connector being at least partially disposed within a container, the first tubular connector being more flexible than the second tubular connector, the second tubular connector having a length that comprises at least 20% of a combined length of the first tubular connector and the second tubular connector. Rotating the drive shaft so as to rotate the first tubular connector and the second tubular connector within the container.

公开(公告)号：US20230152271A1

公开(公告)日：2023-05-18

申请号：US17823696

申请日：2022-08-31

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan M. ROTHBERG ,  Wolfgang HINZ ,  John F. DAVIDSON ,  Antoine M. van OIJEN ,  John LEAMON ,  Martin HUBER ,  Mark James MILGREW ,  James BUSTILLO

IPC: G01N27/414 ,  C12Q1/6874 ,  H01L21/82 ,  C12Q1/6869

CPC classification number: G01N27/4145 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N27/4148 ,  H01L21/82

Abstract: Methods and apparatus relating to FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.

公开(公告)号：US11649479B2

公开(公告)日：2023-05-16

申请号：US17040250

申请日：2019-03-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Yixin Li ,  Bharti Solanki ,  Sheung-Mei Shih ,  Peter Bell ,  Darick Dayne

IPC: C12Q1/70 ,  C12Q1/686 ,  C12Q1/68 ,  C12N15/86

CPC classification number: C12Q1/68 ,  C12Q1/686 ,  C12Q1/70 ,  C12N15/86 ,  C12Q2531/125

Abstract: Provided herein are compositions, methods and uses that relate to an easy, accurate and reliable dual or duplex assay that determines both protein and nucleic acid amounts in a viral preparation in a single container and further determines full versus empty virion content.

公开(公告)号：US20230147242A1

公开(公告)日：2023-05-11

申请号：US17969419

申请日：2022-10-19

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Alexandre VLASSOV ,  Xingwang FANG ,  Madhu JASTI

IPC: C12Q1/6844

CPC classification number: C12Q1/6846 ,  C12Q2527/101

Abstract: The present disclosure generally relates to compositions, kits, and methods for the rapid detection of nucleic acid targets in a sample. In some embodiments, a detection reagent comprising at least two metal indicators is disclosed. In additional embodiments, kits and methodologies for detecting the presence or absence of a target nucleic acid sequence comprising the detection reagent comprising multiple metal indicators are provided.

公开(公告)号：US20230131684A1

公开(公告)日：2023-04-27

申请号：US17929782

申请日：2022-09-06

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Melville Davey ,  Michael Meyer ,  Marcin Sikora ,  Simon Cawley ,  Kirk Pastorian

IPC: C12Q1/6874 ,  G16B30/00

Abstract: A method of estimating a parameter related to sequencing of a sample nucleic acid template includes: receiving signal data relating to nucleotide incorporation events resulting from a series of flows of nucleotides onto an array of wells including (i) a first well containing the sample nucleic acid template and (ii) a plurality of other sample-containing wells; determining sequence information for the sample nucleic acid template using signal data from the first well; and constructing a phase-state model for a set of nucleotide flows that contributed at least in part to the sequence information, wherein the model includes a signal correction parameter that is determined using signal data from the plurality of other sample-containing wells.

公开(公告)号：US20230130038A1

公开(公告)日：2023-04-27

申请号：US17977788

申请日：2022-10-31

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Sanjay K. VASU ,  Chao Yan LIU ,  Jeffrey ROGERS ,  Maria Isabel CISNEROS ,  Jingqui LI ,  Henry C. CHIOU ,  Meredith Bauman JONES

IPC: C07K14/505 ,  C12N5/00 ,  C12N5/0793

Abstract: High-yield mammalian transient expression systems can include a cell culture media (particularly serum free, non-animal derived, and/or chemically defined media) for introducing macromolecules and compounds (e.g., nucleic acid molecules) into cells (e.g., eukaryotic cells). Cells containing such introduced materials can then be cultured in the cell culture media. In particular, the invention allows introduction of nucleic acid molecules (e.g., vectors) into cells (particularly mammalian cells) and expression of proteins encoded by the nucleic acid molecules in the cells. The invention obviates the need to change the cell culture medium each time a different procedure is performed with the cells (e.g., culturing cells vs. transfecting cells). The invention also relates to compositions and kits useful for culturing and transforming/transfecting cells.

公开(公告)号：US20230129295A1

公开(公告)日：2023-04-27

申请号：US18145688

申请日：2022-12-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Wolfgang HINZ ,  John DONOHUE ,  Daniel BEACHAM

IPC: G01N27/414 ,  C09D189/00 ,  G01N27/28 ,  G01N27/30 ,  G01N27/407

Abstract: Various cell analysis systems of the present teachings can measure the electrical and metabolic activity of single, living cells with subcellular addressability and simultaneous data acquisition for between about 10 cells to about 500,000 cells in a single analysis. Various sensor array devices of the present teachings can have sensor arrays with between 20 million to 660 million ChemFET sensors built into a massively paralleled array and can provide for simultaneous measurement of cells with data acquisition rates in the kilohertz (kHz) range. As various ChemFET sensor arrays of the present teachings can detect chemical analytes as well detect changes in cell membrane potential, various cell analysis systems of the present teachings also provide for the controlled chemical and electrical interrogation of cells.