公开(公告)号：US5652337A

公开(公告)日：1997-07-29

申请号：US479666

申请日：1995-06-07

Applicant: Hermann Oppermann ,  Engin Ozkaynak ,  Thangavel Kuberasampath ,  David C. Rueger ,  Roy H. L. Pang ,  Charles M. Cohen

Inventor： Hermann Oppermann ,  Engin Ozkaynak ,  Thangavel Kuberasampath ,  David C. Rueger ,  Roy H. L. Pang ,  Charles M. Cohen

IPC: A01N1/02 ,  A61F2/00 ,  A61K6/00 ,  A61K38/00 ,  A61L27/22 ,  A61L27/24 ,  C07K14/51 ,  C07K14/625 ,  C07K16/22 ,  C12Q1/68 ,  C07K19/51

CPC classification number: C12Q1/6881 ,  A01N1/02 ,  A61L27/227 ,  A61L27/24 ,  C07K14/51 ,  C07K14/625 ,  C07K16/22 ,  A61F2310/00365 ,  A61K38/00 ,  C12Q2600/156 ,  C12Q2600/158 ,  G01N2500/10

Abstract: Disclosed are (1) nucleic acid and amino acid sequences for a novel morphogenic protein; (2) methods for producing and expressing the protein in a biologically active form; and (3) methods for utilizing the protein to induce tissue morphogenesis in a mammal, including methods for increasing a progenitor cell population in a mammal, methods for stimulating progenitor cells to differentiate and maintain their differentiated phenotype in vivo or in vitro, methods for inducing tissue-specific growth in vivo and methods for the replacement of diseased or damaged tissue in vivo.

Abstract translation: 公开了（1）新型形态发生蛋白的核酸和氨基酸序列; （2）以生物活性形式生产和表达蛋白质的方法; 和（3）利用蛋白质诱导哺乳动物组织形态发生的方法，包括增加哺乳动物祖细胞群的方法，用于刺激祖细胞在体内或体外分化和维持其分化表型的方法，诱导方法 体内的组织特异性生长和体内替换患病或受损组织的方法。

公开(公告)号：US5652118A

公开(公告)日：1997-07-29

申请号：US480528

申请日：1995-06-07

Applicant: Engin Ozkaynak ,  Hermann Oppermann

Inventor： Engin Ozkaynak ,  Hermann Oppermann

IPC: A01N1/02 ,  A61F2/00 ,  A61K6/00 ,  A61K38/00 ,  A61K38/17 ,  A61K38/18 ,  A61K48/00 ,  A61L27/22 ,  A61L27/24 ,  C07K14/51 ,  C07K14/625 ,  C07K16/22 ,  C12Q1/68 ,  C12N5/10 ,  C07H21/04 ,  C12N15/12 ,  C12N15/63

CPC classification number: A61K6/0032 ,  A01N1/0226 ,  A61K38/17 ,  A61K38/1703 ,  A61K38/1875 ,  A61L27/227 ,  A61L27/24 ,  C07K14/51 ,  C07K14/625 ,  C07K16/22 ,  C12Q1/6881 ,  A61F2310/00365 ,  A61K48/00 ,  C12Q2600/156 ,  C12Q2600/158 ,  G01N2500/10

Abstract: Disclosed are (1) nucleic acid and amino acid sequences for a novel morphogenic protein; (2) methods for producing and expressing the protein in a biologically active form; and (3) methods for utilizing the protein to induce tissue morphogenesis in a mammal, including methods for increasing a progenitor cell population in a mammal, methods for stimulating progenitor cells to differentiate and maintain their differentiated phenotype in vivo or in vitro, methods for inducing tissue-specific growth in vivo and methods for the replacement of diseased or damaged tissue in vivo.

Abstract translation: 公开了（1）新型形态发生蛋白的核酸和氨基酸序列; （2）以生物活性形式生产和表达蛋白质的方法; 和（3）利用蛋白质诱导哺乳动物组织形态发生的方法，包括增加哺乳动物祖细胞群的方法，用于刺激祖细胞在体内或体外分化和维持其分化表型的方法，诱导方法 体内的组织特异性生长和体内替换患病或受损组织的方法。

公开(公告)号：US5610021A

公开(公告)日：1997-03-11

申请号：US206864

申请日：1994-03-04

Applicant: David C. Rueger ,  William K. Jones ,  Ronald F. Tucker ,  Hermann Oppermann ,  Engin Ozkaynak ,  Kuber T. Sampath

Inventor： David C. Rueger ,  William K. Jones ,  Ronald F. Tucker ,  Hermann Oppermann ,  Engin Ozkaynak ,  Kuber T. Sampath

IPC: A61K9/00 ,  A61K38/00 ,  A61L27/22 ,  C07K16/22 ,  G01N33/53 ,  C07K16/18 ,  G01N33/541 ,  G01N33/68

CPC classification number: A61K9/0024 ,  A61L27/227 ,  C07K16/22 ,  A61K38/00 ,  A61L2430/02

Abstract: Disclosed are novel compositions of osteogenic proteins constituting soluble forms of these proteins, and methods and compositions for distinguishing between soluble and mature forms of these proteins.

Abstract translation: 公开了构成这些蛋白质的可溶形式的成骨蛋白质的新型组合物，以及用于区分这些蛋白质的可溶性和成熟形式的方法和组合物。

公开(公告)号：US5585237A

公开(公告)日：1996-12-17

申请号：US143497

申请日：1993-10-25

Applicant: Hermann Oppermann ,  Haimanti Dorai ,  Paul Kaplan

Inventor： Hermann Oppermann ,  Haimanti Dorai ,  Paul Kaplan

IPC: C12N15/09 ,  C07K14/51 ,  C07K16/28 ,  C07K16/44 ,  C12N1/15 ,  C12N1/19 ,  C12N5/10 ,  C12N15/85 ,  C12P21/02 ,  C12Q1/68 ,  C12N15/64

CPC classification number: C12N15/85 ,  C07K14/51 ,  C07K16/2863 ,  C07K16/44 ,  C12N2800/108 ,  C12N2830/002 ,  C12N2830/55 ,  C12N2840/20 ,  C12N2840/44

Abstract: Disclosed herein are improved methods and compositions for achieving enhanced protein production expressed from non-native gene constructs, including single chain sFv and derivative sequences. The methods and compositions are particularly useful for creating stably transfected, contitutively expressing immortalized mammalian cell lines that exhibit high recombinant protein productivity while maintaining a low copy number per cell of the non-native recombinant DNA sequence encoding the protein of interest.

Abstract translation: 本文公开了用于实现由非天然基因构建体（包括单链sFv和衍生序列）表达的增强的蛋白质产生的改进的方法和组合物。 所述方法和组合物特别可用于产生显示高重组蛋白质生产力的稳定转染的，替代表达的永生化哺乳动物细胞系，同时保持编码目标蛋白质的非天然重组DNA序列的每个细胞的拷贝数低。

公开(公告)号：US5534254A

公开(公告)日：1996-07-09

申请号：US133804

申请日：1993-10-07

Applicant: James S. Huston ,  L. L. Houston ,  David B. Ring ,  Hermann Oppermann

Inventor： James S. Huston ,  L. L. Houston ,  David B. Ring ,  Hermann Oppermann

IPC: C12N15/09 ,  A61K39/395 ,  A61K49/00 ,  A61K51/00 ,  A61K51/08 ,  A61P35/00 ,  C07H21/04 ,  C07K16/22 ,  C07K16/28 ,  C07K16/30 ,  C07K16/32 ,  C07K16/46 ,  C07K19/00 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N15/13 ,  C12N15/62 ,  C12N15/66 ,  C12P21/02 ,  C12P21/08 ,  C12R1/19 ,  A61K39/00

CPC classification number: C07K16/32 ,  C07K16/22 ,  C07K16/30 ,  C07K16/3015 ,  C07K16/3069 ,  A61K2039/505 ,  C07K2317/565 ,  C07K2317/567 ,  C07K2317/622 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/33 ,  C07K2319/55

Abstract: Disclosed is a formulation for targeting an epitope on an antigen expressed in a mammal. The formulation comprises a pharmaceutically acceptable carrier together with a dimeric biosynthetic construct for binding at least one preselected antigen. The biosynthetic construct contains two polypeptide chains, each of which define single-chain Fv (sFv) binding proteins and have C-terminal tails that facilitate the crosslinking of two sFv polypeptides. The resulting dimeric constructs have a conformation permitting binding of a said preselected antigen by the binding site of each said polypeptide chain when administered to said mammal. The formulation has particular utility in in vivo imaging and drug targeting experiments.

公开(公告)号：US5482858A

公开(公告)日：1996-01-09

申请号：US139171

申请日：1993-10-19

Applicant: James S. Huston ,  Hermann Oppermann

Inventor： James S. Huston ,  Hermann Oppermann

IPC: C07K16/00 ,  C07K16/46 ,  C12N15/62 ,  C12P21/08 ,  C12N1/21 ,  C12N5/10 ,  C12N15/09

CPC classification number: C07K16/464 ,  C07K16/00 ,  C12N15/62 ,  C07K2317/24 ,  C07K2317/622 ,  C07K2317/624 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/705 ,  Y10S424/80

Abstract: Disclosed are a family of synthetic proteins having binding affinity for a preselected antigen, and multifunctional proteins having such affinity. The proteins are characterized by one or more sequences of amino acids constituting a region which behaves as a biosynthetic antibody binding site (BABS). The sites comprise V.sub.H -V.sub.L or V.sub.L -V.sub.H -like single chains wherein the V.sub.H and V.sub.L -like sequences are attached by a polypeptide linker, or individual V.sub.H or V.sub.L -like domains. The binding domains comprise linked CDR and FR regions, which may be derived from separate immunoglobulins. The proteins may also include other polypeptide sequences which function, e.g., as an enzyme, toxin, binding site, or site for attachment to an immobilization media or radioactive atom. Methods are disclosed for producing the proteins, for designing BABS having any specificity that can be elicited by in vivo generation of antibody, for producing analogs thereof, and for producing multifunctional synthetic proteins which are self-targeted by virtue of their binding site region.

Abstract translation: 公开了对预选抗原具有结合亲和力的合成蛋白质家族，以及具有这种亲和力的多功能蛋白质。 蛋白质的特征在于构成表现为生物合成抗体结合位点（BABS）的区域的一个或多个氨基酸序列。 所述位点包含VH-VL或VL-VH样单链，其中VH和VL样序列通过多肽接头或单独的VH或VL样结构域连接。 结合域包含连接的CDR和FR区，其可以衍生自单独的免疫球蛋白。 蛋白质还可以包括其它多肽序列，其功能例如作为酶，毒素，结合位点或用于附着到固定化介质或放射性原子的位点。 公开了用于制备蛋白质的方法，用于设计具有可通过体内产生抗体引起的任何特异性的BABS，用于产生其类似物，以及用于生产通过其结合位点区域自身靶向的多功能合成蛋白质。

公开(公告)号：US5354557A

公开(公告)日：1994-10-11

申请号：US993387

申请日：1992-12-18

Applicant: Hermann Oppermann ,  Engin Ozkaynak ,  Thangavel Kuberasampath ,  David C. Rueger ,  Roy H. L. Pang

Inventor： Hermann Oppermann ,  Engin Ozkaynak ,  Thangavel Kuberasampath ,  David C. Rueger ,  Roy H. L. Pang

IPC: A61C8/00 ,  A61F2/00 ,  A61K6/00 ,  A61K9/00 ,  A61K38/00 ,  A61K38/18 ,  A61L27/22 ,  A61L27/24 ,  A61L27/34 ,  A61L27/36 ,  A61L27/56 ,  C07K14/51 ,  C07K13/00 ,  A61K37/00 ,  C07K15/00 ,  C12P21/00

CPC classification number: A61L27/24 ,  A61K38/1875 ,  A61K9/0024 ,  A61L27/227 ,  A61L27/34 ,  A61L27/56 ,  C07K14/51 ,  A61C8/0006 ,  A61F2310/00365 ,  A61L2430/02

Abstract: Disclosed are (1) osteogenic devices comprising a matrix containing substantially pure natural-sourced mammalian osteogenic protein; (2) DNA and amino acid sequences for novel polypeptide chains useful as subunits of dimeric osteogenic proteins; (3) vectors carrying sequences encoding these novel polypeptide chains and host cells transfected with these vectors; (4) methods of producing these polypeptide chains using recombinant DNA technology; (5) antibodies specific for these novel polypeptide chains; (6) osteogenic devices comprising these recombinantly produced proteins in association with an appropriate carrier matrix; and (7) methods of using the osteogenic devices to mimic the natural course of endochondral bone formation in mammals.

Abstract translation: 公开的是（1）包含含有基本上纯天然来源的哺乳动物成骨蛋白质的基质的成骨装置; （2）可用作二聚成骨蛋白亚基的新型多肽链的DNA和氨基酸序列; （3）携带编码这些新型多肽链的序列的载体和用这些载体转染的宿主细胞; （4）使用重组DNA技术生产这些多肽链的方法; （5）对这些新型多肽链具有特异性的抗体; （6）包含这些重组产生的蛋白质与合适的载体基质相结合的成骨装置; 和（7）使用成骨装置来模拟哺乳动物软骨内骨形成的自然过程的方法。

公开(公告)号：US5258494A

公开(公告)日：1993-11-02

申请号：US995345

申请日：1992-12-22

Applicant: Hermann Oppermann ,  Thangavel Kuberasampath ,  David C. Rueger ,  Engin Ozkaynak

Inventor： Hermann Oppermann ,  Thangavel Kuberasampath ,  David C. Rueger ,  Engin Ozkaynak

IPC: A61C8/00 ,  A61F2/00 ,  A61K9/00 ,  A61K38/00 ,  A61L27/22 ,  A61L27/24 ,  A61L27/34 ,  A61L27/36 ,  A61L27/56 ,  C07K14/51 ,  C12N15/18 ,  C07K5/00 ,  A61K37/02 ,  C07K7/00 ,  C07K15/00

CPC classification number: A61K9/0024 ,  A61L27/227 ,  A61L27/24 ,  A61L27/34 ,  A61L27/3608 ,  A61L27/365 ,  A61L27/3654 ,  A61L27/56 ,  C07K14/51 ,  A61C8/0006 ,  A61F2310/00365 ,  A61K38/00 ,  A61L2430/02 ,  Y10S530/84

Abstract: Disclosed are 1) osteogenic devices comprising a matrix containing osteogenic protein and methods of inducing endochondral bone growth in mammals using the devices; 2) amino acid sequence data, amino acid composition, solubility properties, structural features, homologies and various other data characterizing osteogenic proteins, 3) methods of producing osteogenic proteins using recombinant DNA technology, and 4) osteogenically and chondrogenically active synthetic protein constructs.

公开(公告)号：US09815146B2

公开(公告)日：2017-11-14

申请号：US14001486

申请日：2012-02-28

Applicant: Hermann Oppermann ,  Matthias Hutter ,  Christian Ehrhardt

Inventor： Hermann Oppermann ,  Matthias Hutter ,  Christian Ehrhardt

IPC: B23K35/26 ,  B23K35/30 ,  B23K35/36 ,  B23K35/362 ,  B23K35/02 ,  B23K35/32 ,  H01L23/00

CPC classification number: B23K35/025 ,  B23K35/0244 ,  B23K35/26 ,  B23K35/262 ,  B23K35/264 ,  B23K35/3006 ,  B23K35/3013 ,  B23K35/302 ,  B23K35/3033 ,  B23K35/322 ,  B23K35/3612 ,  B23K35/3613 ,  B23K35/362 ,  H01L24/29 ,  H01L24/32 ,  H01L2224/29294 ,  H01L2224/29309 ,  H01L2224/29311 ,  H01L2224/29318 ,  H01L2224/29339 ,  H01L2224/29344 ,  H01L2224/29347 ,  H01L2224/29355 ,  H01L2224/29364 ,  H01L2224/29369 ,  H01L2224/29499 ,  H01L2224/32507 ,  H01L2924/00013 ,  H01L2924/01029 ,  H01L2924/01327 ,  H01L2924/13055 ,  H01L2924/15787 ,  Y10T403/479 ,  H01L2924/00014 ,  H01L2224/13099 ,  H01L2224/13599 ,  H01L2224/05599 ,  H01L2224/05099 ,  H01L2224/29099 ,  H01L2224/29599 ,  H01L2924/00

Abstract: The invention relates to a paste, preferably for joining components of power electronics modules, the paste comprising a solder powder, a metal powder and a binder, wherein the binder binds solder powder and metal powder before a first heating. According to the invention, the binder is free of flux or is a flux having only low activation. In this way, a joining layer which exhibits only few included voids and good mechanical and electrical stability can be provided between a first and a second component.

公开(公告)号：US08673773B2

公开(公告)日：2014-03-18

申请号：US12743009

申请日：2008-11-14

Applicant: Hermann Oppermann ,  Lothar Dietrich ,  Gunter Engelmann ,  Wolf Jürgen

Inventor： Hermann Oppermann ,  Lothar Dietrich ,  Gunter Engelmann ,  Wolf Jürgen

IPC: H01L21/4763

CPC classification number: C25F3/02 ,  C25D5/022 ,  C25D5/48 ,  H01L21/321

Abstract: A method for producing a nanoporous layer comprises applying a plating base with adhesion strengthening onto a substrate, depositing a layer made of gold and silver onto the substrate, the composition being in the range of 20% to 40% gold and 80% to 60% silver, and selectively removing the silver in order to produce a nanoporous gold layer.

Abstract translation: 一种纳米多孔层的制造方法，其特征在于，在基材上涂敷具有附着强化的镀敷基材，在基材上沉积由金，银构成的层，金属含量在20〜40％的范围，80〜60％ 银，并且选择性地除去银以产生纳米多孔金层。