公开(公告)号：US11353461B2

公开(公告)日：2022-06-07

申请号：US16312800

申请日：2017-06-19

Applicant: WATERS TECHNOLOGIES CORPORATION

Inventor： Darryl W. Brousmiche ,  Matthew A. Lauber

IPC: G01N33/00 ,  G01N33/58 ,  G01N33/50 ,  G01N33/68

Abstract: Methods for derivatization of biomolecules including glycans or other biopolymers with one or more fluorescent, MS active compounds by reductive amination or rapid tagging in order to produce derivatized glycan having a pKa>7 and between about 200 Å2 and about 1000 Å2 of nonpolar surface area are described.

公开(公告)号：US20220081468A1

公开(公告)日：2022-03-17

申请号：US17477331

申请日：2021-09-16

Applicant: Waters Technologies Corporation

Inventor： Andrew Wyatt Schmudlach ,  Susan Rzewuski ,  Matthew A. Lauber ,  Yeliz Tunc Sarisozen ,  Nicole L. Lawrence ,  Darryl W. Brousmiche

IPC: C07K1/22 ,  C07C277/06 ,  C07C227/40 ,  B01J20/285 ,  B01J20/28 ,  B01J20/283 ,  B01J20/26 ,  B01J20/10 ,  C12N7/00 ,  B01D15/34 ,  B01J20/281

Abstract: The present disclosure is directed to methods for performing size exclusion chromatography. Embodiments of the present disclosure feature methods for improving separations of proteinaceous analytes in size exclusion chromatography, for example, by using low concentrations of amino acids or derivatives thereof in the mobile phase.

公开(公告)号：US20210396632A1

公开(公告)日：2021-12-23

申请号：US17466389

申请日：2021-09-03

Applicant: Waters Technologies Corporation

Inventor： Wenjing Li ,  Beatrice Muriithi ,  Anna Boardman ,  Matthew A. Lauber

IPC: G01N1/40 ,  G01N1/28 ,  G01N1/31 ,  C12M1/40

Abstract: The present disclosure relates to a kit for sample preparation, the kit including a solid support surface with a polymer coating covering the solid support surface, wherein the polymer coating reduces undesired interactions between the sample and the solid support surface, a buffer comprising arginine and methionine, and a vessel for containing the solid support surface and the buffer.

公开(公告)号：US11150248B2

公开(公告)日：2021-10-19

申请号：US16314554

申请日：2017-06-19

Applicant: WATERS TECHNOLOGIES CORPORATION

Inventor： Matthew A. Lauber

IPC: G01N33/68 ,  C07K1/13 ,  C07K1/36 ,  G01N1/40 ,  G01N1/28 ,  G01N30/06 ,  G01N30/78 ,  G01N30/02 ,  B01D15/30 ,  B01D63/16

Abstract: Methods for preparing labeled glycosylamines from a complex matrix are provided. The methodology includes the steps of: denaturing glycoproteins in a complex matrix to form a denatured complex matrix mixture; loading the denatured complex matrix mixture onto a MWCO filtration device; adding a glycosidase enzymatic solution onto the MWCO filtration device to form a deglycosylated complex matrix mixture comprising glycosylamines; collecting glycosylamines released from the MWCO filtration device; and derivatizing glycosylamines with a rapid tagging reagent to form a plurality of labeled glycosylamines suitable for detection in various liquid chromatography systems and detectors.

公开(公告)号：US11092574B2

公开(公告)日：2021-08-17

申请号：US14677719

申请日：2015-04-02

Applicant: Waters Technologies Corporation

Inventor： Matthew A. Lauber ,  Stephan M. Koza ,  Pamela C. Iraneta ,  Kevin D. Wyndham

IPC: G01N30/06 ,  G01N30/72 ,  B01D15/30 ,  C08F220/54 ,  C08F220/56 ,  B01J20/28 ,  B01J20/32 ,  C08F222/38 ,  B01J20/286 ,  G01N30/00 ,  G01N30/52 ,  G01N30/02 ,  C08F230/08 ,  C08L33/14

Abstract: The invention relates to poly-amide bonded hydrophilic interaction chromatography (HILIC) stationary phases and novel HILIC methods for use in the characterization of large biological molecules modified with polar groups, known to those skilled in the art as glycans. The invention particularly provides novel, poly-amide bonded materials designed for efficient separation of large biomolecules, e.g. materials having a large percentage of larger pores (i.e. wide pores). Furthermore, the invention advantageously provides novel HILIC methods that can be used in combination with the stationary phase materials described herein to effectively separate protein and peptide glycoforms by eliminating previously unsolved problems, such as on-column aggregation of protein samples, low sensitivity of chromatographic detection of the glycan moieties, and low resolution of peaks due to restricted pore diffusion and long intra/inter-particle diffusion distances.

公开(公告)号：US11035832B2

公开(公告)日：2021-06-15

申请号：US16312760

申请日：2017-06-19

Applicant: WATERS TECHNOLOGIES CORPORATION

Inventor： Darryl W. Brousmiche ,  Matthew A. Lauber

IPC: G01N30/06 ,  B01D15/12 ,  B01D15/32 ,  B01D15/38 ,  G01N33/58 ,  G01N30/04 ,  G01N30/72 ,  H01J49/16 ,  G01N30/88

Abstract: Solutions, detection methods and chromatographic systems are provided for electrospray ionization of glycans modified with amphipathic, strongly basic moieties. The solutions for use in electrospray ionization comprise a plurality of glycans having an amphipathic moiety, a basic residue of pKa>5 and a Log P value between 1 and 3, and one or more volatile components selected from the group consisting of an amine, ammonia, ammonia salt, diethylamine, or trimethylamine. The solutions also have a pH between about 3 to about 6, and ionic strength of between about 0 mM to about 500 mM. The solutions are useful in detecting modified glycans in electrospray ionization and in various chromatographic systems.

公开(公告)号：US10857516B2

公开(公告)日：2020-12-08

申请号：US15921811

申请日：2018-03-15

Applicant: WATERS TECHNOLOGIES CORPORATION

Inventor： Matthew A. Lauber ,  Daniel P. Walsh

IPC: B01J20/286 ,  B01J20/32 ,  C07F7/08 ,  B01J20/289 ,  B01J20/10 ,  B01J20/22 ,  B01J20/283

Abstract: Provided herein are stationary phase compositions comprising a chromatographic surface of porous or non-porous core material comprising a surface modifier for use in chromatographic separations.

公开(公告)号：US20200332028A1

公开(公告)日：2020-10-22

申请号：US16095919

申请日：2017-04-21

Applicant: Waters Technologies Corporation

Inventor： Matthew A. Lauber ,  Scott A. McCall ,  Babajide Okandeji ,  Pamela C. Iraneta

IPC: C08B37/00 ,  B01D15/32 ,  B01D15/38 ,  B01D15/36 ,  G01N33/58

Abstract: The present invention provides novel methods for the chromatographic analysis of glycans using high purity chromatographic materials comprising a chromatographic surface wherein the chromatographic surface comprises a hydrophobic surface group and one or more ionizable modifier and a labeling reagent which is capable of providing amphipathic and strongly basic labeling moieties to a sample to be analyzed.

公开(公告)号：US20200271629A1

公开(公告)日：2020-08-27

申请号：US16800454

申请日：2020-02-25

Applicant: Waters Technologies Corporation

Inventor： Matthew A. Lauber ,  Martin Gilar ,  Mathew H. DeLano ,  Michael Donegan

IPC: G01N30/26 ,  B01D15/22 ,  B01D15/38 ,  B01D15/36 ,  B01D15/18

Abstract: The present disclosure relates to the determination of analytes in a sample using chromatography. The present disclosure provides methods of separating an analyte from a sample. The method includes introducing a sample comprising the analytes into a chromatographic system. The chromatographic system has a flow path disposed in an interior of the chromatographic system, at least a portion of the flow path having an active coating, and a chromatographic column having a stationary phase material in an interior of the chromatographic column that facilitates separation of the analytes in the sample through interaction with at least one analyte in the sample. The active coating is selected to interact with at least one analyte in the sample through (1) a repulsive force, (2) a secondary interaction, or (3) a retention mechanism distinct from the interaction with the stationary phase material.

公开(公告)号：US20200016511A1

公开(公告)日：2020-01-16

申请号：US16506801

申请日：2019-07-09

Applicant: WATERS TECHNOLOGIES CORPORATION

Inventor： Matthew A. Lauber ,  Babajide O. Okandeji

IPC: B01D15/18 ,  B01D15/36 ,  B01D15/14

Abstract: In various aspects, the present disclosure pertains to materials (e.g., kits, column assemblies, liquid chromatography systems, etc.) methods for performing liquid chromatography that employ a first column (e.g., a trapping column) and a second column (e.g., an analytical column). The first column comprises a first chromatographic material having a first chromatographic surface that comprises first hydrophobic surface groups and first ionizable surface groups having a first pKa value. The second column comprises a second chromatographic material having a second chromatographic surface that comprises second hydrophobic surface groups and (a) permanently ionized surface groups or (b) second ionizable surface groups having a second pKa value. The first hydrophobic surface groups have a hydrophobicity that is less than a hydrophobicity of the second hydrophobic surface groups. Moreover, where the second chromatographic surface comprises second ionizable surface groups, the first pKa value may differ from the second pKa value by 1-12 units.