METHOD FOR TREATING CELLULOSE-CONTAINING BIOMASS
    72.
    发明申请
    METHOD FOR TREATING CELLULOSE-CONTAINING BIOMASS 审中-公开
    用于处理含细菌生物质的方法

    公开(公告)号:US20160251690A1

    公开(公告)日:2016-09-01

    申请号:US15027617

    申请日:2014-09-09

    CPC classification number: C12P19/14 C12M45/02 C12P19/02 C12P2201/00 C13K1/02

    Abstract: A treatment method for biomass, which is a pretreatment method including continuously performing hydrothermal treatment using a screw extruder to produce a biomass composition for saccharification from cellulose-containing biomass and containing only a small quantity of furfural, the treatment method sequentially including: pulverizing the cellulose-containing biomass so as to have a maximum grain size of 1,000 μm or less and adjusting a water content ratio thereof to from 30% to 80%; performing hydrothermal treatment in which an element including a seal ring and at least one set of a kneading disc and/or a left-hand screw is arranged immediately upstream of the seal ring, at a temperature of (1) from 150° C. to 200° C. or (2) from 200° C. to 215° C. for 0.1 minute to 15 minutes while performing kneading pulverization having a grinding effect; and cooling a treated product to 100° C. or less to recover the treated product.

    Abstract translation: 一种生物质处理方法,其特征在于,所述预处理方法包括使用螺杆挤出机连续进行水热处理,以生产由含纤维素生物质糖化并仅含有少量糠醛的生物质组合物,所述处理方法依次包括:将所述纤维素 含有生物质,使其最大粒径为1000μm以下,将其含水率调整为30〜80%。 进行水热处理,其中包括密封环和至少一组捏合盘和/或左旋螺杆的元件紧邻密封环的上游,在(1)从150℃到 200℃或(2)从200℃至215℃进行0.1分钟至15分钟,同时进行具有研磨效果的捏合粉碎; 并将处理的产物冷却至100℃或更低以回收经处理的产物。

    HYDROTHERMAL-MECHANICAL CONVERSION OF LIGNOCELLULOSIC BIOMASS TO ETHANOL OR OTHER FERMENTATION PRODUCTS
    73.
    发明申请
    HYDROTHERMAL-MECHANICAL CONVERSION OF LIGNOCELLULOSIC BIOMASS TO ETHANOL OR OTHER FERMENTATION PRODUCTS 审中-公开
    木糖醇生物质对乙醇或其他发酵产品的水力机械转化

    公开(公告)号:US20160244788A1

    公开(公告)日:2016-08-25

    申请号:US15047608

    申请日:2016-02-18

    Abstract: A low-cost process is provided to render lignocellulosic biomass accessible to cellulase enzymes, to produce fermentable sugars. Some variations provide a process to produce ethanol from lignocellulosic biomass (such as sugarcane bagasse or corn stover), comprising introducing a lignocellulosic biomass feedstock to a single-stage digestor; exposing the feedstock to a reaction solution comprising steam or liquid hot water within the digestor, to solubilize the hemicellulose in a liquid phase and to provide a cellulose-rich solid phase; refining the cellulose-rich solid phase, together with the liquid phase, in a mechanical refiner, thereby providing a mixture of refined cellulose-rich solids and the liquid phase; enzymatically hydrolyzing the mixture in a hydrolysis reactor with cellulase enzymes, to generate fermentable sugars; and fermenting the fermentable sugars to produce ethanol. Many alternative process configurations are described. The disclosed processes may be employed for other fermentation products.

    Abstract translation: 提供了一种低成本的方法来使木质纤维素生物质可获得纤维素酶,以产生可发酵的糖。 一些变型提供了从木质纤维素生物质(例如甘蔗渣或玉米秸秆)生产乙醇的方法,包括将木质纤维素生物质原料引入单级消化器; 将原料暴露于消化器内包含蒸汽或液体热水的反应溶液中,以将半纤维素溶解在液相中并提供富含纤维素的固相; 在机械磨浆机中将富含纤维素的固相与液相一起精炼,从而提供精制富含纤维素的固体和液相的混合物; 在水解反应器中用纤维素酶酶水解水解产生可发酵糖; 并发酵可发酵糖以产生乙醇。 描述了许多替代的工艺配置。 所公开的方法可用于其它发酵产物。

    METHOD OF PRODUCING A SUGAR LIQUID
    77.
    发明申请
    METHOD OF PRODUCING A SUGAR LIQUID 审中-公开
    生产糖液的方法

    公开(公告)号:US20160208300A1

    公开(公告)日:2016-07-21

    申请号:US14913404

    申请日:2014-08-21

    Abstract: A method of producing a sugar liquid includes steps (1) providing a pretreatment product of a first cellulose-containing biomass; (2) adding a second cellulose-containing biomass or a pretreatment product thereof in an amount ranging from 1 to 50% based on the solid content weight of the first cellulose-containing biomass pretreatment product of step (1), and carrying out hydrolysis by a filamentous fungus-derived cellulase; and (3) subjecting a hydrolysate of step (2) to solid-liquid separation to obtain a solution component and a hydrolysis residue, and filtering the solution component through an ultrafiltration membrane to recover the filamentous fungus-derived cellulase as a retentate and recover a sugar liquid as a permeate.

    Abstract translation: 制备糖液的方法包括步骤(1),提供第一含纤维素生物质的预处理产物; (2)基于步骤(1)的第一含纤维素的生物质预处理产物的固体含量,添加第一含纤维素的生物质或其预处理产物,其量为1至50%,并通过 丝状真菌衍生的纤维素酶; (3)将步骤(2)的水解产物进行固液分离,得到溶液成分和水解残渣,并通过超滤膜过滤溶液成分,回收丝状真菌衍生的纤维素酶作为滞留物,并回收 糖液作为渗透物。

    METHODS OF PROCESSING LIGNOCELLULOSIC BIOMASS USING SINGLE-STAGE AUTOHYDROLYSIS PRETREATMENT AND ENZYMATIC HYDROLYSIS
    79.
    发明申请
    METHODS OF PROCESSING LIGNOCELLULOSIC BIOMASS USING SINGLE-STAGE AUTOHYDROLYSIS PRETREATMENT AND ENZYMATIC HYDROLYSIS 有权
    使用单级自动溶解预处理和酶水解处理线粒体生物量的方法

    公开(公告)号:US20160160253A1

    公开(公告)日:2016-06-09

    申请号:US14908913

    申请日:2014-02-05

    Applicant: INBICON A/S

    Abstract: Methods of processing lignocellulosic biomass to fermentable sugars are provided which rely on hydrothermal pretreatment. Soft lignocellulosic biomass feedstock is pretreated in a single-stage pressurized hydrothermal pretreatment to very low severity. The pre-treated biomass is hydrolysed, typically as a whole slurry, using enzymatic hydrolysis catalysed by an enzyme mixture comprising endoglucanase, exoglucanase, β-glucosidase, endoxylanase, and β-xylosidase activities at activity levels in nkat/g glucan of endoglucanase of at least 1100, exoglucanase of at least 280, β-glucosidase of at least 3000, endoxylanase of at least 1400, and β-xylosidase of at least 75, so as to produce a hydrolysate in which the yield of C5 monomers is at least 55% of the original xylose and arabinose content of the feedstock prior to pretreatment.

    Abstract translation: 提供了依靠水热预处理将木质纤维素生物质加工成可发酵糖的方法。 软木质纤维素生物质原料在单级加压水热预处理中预处理至非常低的严重性。 使用由含有内切葡聚糖酶,外切葡聚糖酶,葡萄糖苷酶,内切木聚糖酶和β-木糖苷酶活性的酶混合物催化的酶水解,预处理的生物质通常作为整体浆液水解,其活性水平在内切葡聚糖酶的nkat / g葡聚糖 至少为1100,至少280的外切葡聚糖酶,至少为3000的葡萄糖苷酶,至少为1400的内切木聚糖酶,至少为75的木糖苷酶,以及至少75的β-木糖苷酶,以产生其中C5单体的产率为 在预处理之前,原料中原始木糖和阿拉伯糖含量的至少55%。

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