公开(公告)号：US20110256580A1

公开(公告)日：2011-10-20

申请号：US13141727

申请日：2009-12-16

Applicant: Sanggoo Kim ,  Jin-Woo Jung ,  Chan-Soo Park

Inventor： Sanggoo Kim ,  Jin-Woo Jung ,  Chan-Soo Park

IPC: G01N21/75 ,  C12Q1/02 ,  G01N30/16 ,  G01N21/64

CPC classification number: G01N30/6095 ,  B01L3/502784 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0883 ,  B01L2400/0487 ,  G01N30/7233 ,  G01N33/54366 ,  G01N33/6845 ,  Y10T436/143333

Abstract: The present invention relates to a method for screening a new drug candidate using a liquid chromatography/microfluidic device/mass spectrometry system, and to a liquid chromatography/microfluidic device/mass spectrometry system. The present invention involves the detection of an interaction between molecules on a real-time basis through adjustment of a microreaction between traces of natural material or synthesized new drug candidates and a target material (protein or cell, etc.), thus developing materials for new drug candidates at a lower cost and with high efficiency, while improving quality of life and reducing medical costs. The present invention can be valuably used in increasing new scientific technology through the convergence of nanotechnology, biotechnology, and analytical chemistry technology.

Abstract translation: 本发明涉及使用液相色谱/微流体装置/质谱系统以及液相色谱/微流体装置/质谱系统筛选新的候选药物的方法。 本发明涉及通过调节天然材料痕迹或合成新药物候选物和靶材料（蛋白质或细胞等）之间的微反应来实时检测分子之间的相互作用，从而开发新的材料 药物候选人以较低的成本和高效率，同时提高生活质量和降低医疗成本。 通过纳米技术，生物技术和分析化学技术的融合，本发明可以有价值地用于增加新的科学技术。

公开(公告)号：US07889034B2

公开(公告)日：2011-02-15

申请号：US11568024

申请日：2005-11-29

Applicant: Byoung-Seob Lee ,  Dong-Lak Kim ,  Hyun-Sik Kim ,  Jong-Shin Yoo

Inventor： Byoung-Seob Lee ,  Dong-Lak Kim ,  Hyun-Sik Kim ,  Jong-Shin Yoo

IPC: H01F6/00 ,  G01V3/00

CPC classification number: G01R33/3815

Abstract: Disclosed is a method for designing a superconducting magnet for generating high magnetic fields with high uniformity for controlling a stray field to be within an allowable range and acquiring structural and magnetic stability by optimizing the arrangement of positions and shapes of coils configuring the superconducting magnet. Volumes of a main coil and a shielding coil are set to be variables, and the critical value of a wires related on the current and magnetic field, the heat transfer depth, and the quench strain are defined to be restriction conditions so that linear programming is applied to determine an initial shape of the shielding coil and division of the main coil based on the sum of total volumes, that is, an objective function. The initial shapes of the main coil and the shielding coil determined through the linear programming are revised and the shape of a shimming coil is determined by using non-linear programming based on the objective function.

Abstract translation: 公开了一种设计用于产生高均匀性的高磁场的超导磁体的方法，用于通过优化构成超导磁体的线圈的位置和形状的布置来优化将杂散磁场控制在允许范围内并获得结构和磁稳定性。 主线圈和屏蔽线圈的体积被设置为变量，并且将与电流和磁场相关的导线的临界值，传热深度和骤冷应变定义为限制条件，使得线性规划是 用于确定屏蔽线圈的初始形状和基于总体积的总和即目标函数的主线圈的划分。 修改通过线性规划确定的主线圈和屏蔽线圈的初始形状，并且通过使用基于目标函数的非线性规划来确定匀场线圈的形状。

公开(公告)号：US07713660B2

公开(公告)日：2010-05-11

申请号：US12084103

申请日：2006-10-20

Applicant: Hae Jin Kim ,  Jin Bae Lee

Inventor： Hae Jin Kim ,  Jin Bae Lee

IPC: H01M4/50 ,  H01M4/58 ,  C01G45/02 ,  C01G47/00 ,  C01G57/00

CPC classification number: C01G45/02 ,  B82Y30/00 ,  C01P2002/72 ,  C01P2004/02 ,  C01P2004/04 ,  C01P2004/13 ,  C01P2004/16 ,  C01P2004/60 ,  C01P2006/40 ,  C01P2006/60 ,  C04B35/053 ,  C04B35/111 ,  C04B38/00 ,  C04B2111/00008 ,  C30B7/00 ,  C30B29/16 ,  C30B29/602 ,  H01M4/0404 ,  H01M4/043 ,  H01M4/505 ,  H01M4/621 ,  H01M4/625 ,  H01M10/052 ,  Y02E60/122 ,  Y02P70/54 ,  C04B38/0054

Abstract: The present invention relates to methods for manufacturing manganese oxide nanotubes/nanorods using an anodic aluminum oxide (AAO) template. In the inventive methods, the manganese oxide nanotubes/nanorods are manufactured in mild conditions using only a manganese oxide precursor and an anodic aluminum oxide template without using any solvent. The nanotubes/nanorods having uniform size can be easily obtained by adsorbing the manganese oxide precursor onto the surface of the anodic aluminum oxide template by a vacuum forming process using a vacuum filtration apparatus so as to maintain the shape of nanotubes/nanorods and drying the manganese oxide nanotubes. The manganese oxide nanotubes/nanorods made according to the inventive methods can be used as economic hydrogen reservoirs, the electrode of lithium secondary batteries, or the energy reservoirs of vehicles or other transport means.

Abstract translation: 本发明涉及使用阳极氧化铝（AAO）模板制造氧化锰纳米管/纳米棒的方法。 在本发明的方法中，仅使用锰氧化物前体和阳极氧化铝模板，而不使用任何溶剂，在温和条件下制造氧化锰纳米管/纳米棒。 通过使用真空过滤装置的真空成型方法将锰氧化物前体吸附到阳极氧化铝模板的表面上，可以容易地获得具有均匀尺寸的纳米管/纳米棒，以保持纳米管/纳米棒的形状并干燥锰 氧化物纳米管。 根据本发明方法制备的氧化锰纳米管/纳米棒可以用作经济氢气储存器，锂二次电池的电极，或车辆的能量储存器或其他运输工具。

公开(公告)号：US07579460B2

公开(公告)日：2009-08-25

申请号：US11307457

申请日：2006-02-08

Applicant: Yeong Hee Ahn ,  Jong Shin Yoo ,  Soohyun Kim

Inventor： Yeong Hee Ahn ,  Jong Shin Yoo ,  Soohyun Kim

IPC: C08B37/08 ,  C07B37/10 ,  C07B37/00 ,  C07H3/06

CPC classification number: C07H5/06

Abstract: The present invention relates to the method for tagging of carbohydrates with active methylene compound. Particularly, it relates to the method for tagging of carbohydrates with active methylene compound comprising the step of preparing carbohydrate conjugate in which carbohydrate and methylene compound are combined by mixing carbohydrate mixture and methylene compound under aqueous polar aprotic solvent containing amine base catalyst. The tagging method of the present invention does not need many kinds of chemical reagent and the reactions can be taken even in the presence of certain amount of impurities. So, it can be used for the analysis of oligosaccharide present in the various kinds of samples.

Abstract translation: 本发明涉及用活性亚甲基化合物标记碳水化合物的方法。 特别涉及用活性亚甲基化合物标记碳水化合物的方法，包括制备碳水化合物缀合物的步骤，其中碳水化合物和亚甲基化合物通过混合碳水化合物混合物和亚甲基化合物在含有胺基催化剂的极性非质子溶剂的水溶液下混合。 本发明的标记方法不需要多种化学试剂，即使在存在一定量的杂质的情况下也可以进行反应。 因此，可用于各种样品中存在的寡糖的分析。

公开(公告)号：US20090138206A1

公开(公告)日：2009-05-28

申请号：US12282440

申请日：2007-02-23

Applicant: Gun Wook Park ,  Kyung-Hoon Kwon ,  Jin Young Kim ,  Jong Shin Yoo ,  Young Mok Park ,  Seung Il Kim

Inventor： Gun Wook Park ,  Kyung-Hoon Kwon ,  Jin Young Kim ,  Jong Shin Yoo ,  Young Mok Park ,  Seung Il Kim

IPC: G06F19/00 ,  G01N33/68

CPC classification number: G01N33/6848 ,  H01J49/004

Abstract: The present invention relates to a method of analyzing protein modification. The method of invention for analyzing protein distribution and characteristics on one-dimensional gel provides the way to analyze proteins of samples on one-dimensional gel quantitatively and provides information on interactions among proteins and further can be effectively used for the development of a novel diagnostic and therapeutic method for a disease by screening a disease marker protein.

Abstract translation: 本发明涉及分析蛋白质修饰的方法。 本发明用于分析一维凝胶上的蛋白质分布和特征的方法提供了定量分析一维凝胶上样品蛋白质的方法，提供蛋白质间相互作用信息，进一步有效地用于开发新型诊断和 通过筛选疾病标记蛋白来治疗疾病的治疗方法。

公开(公告)号：US20090008549A1

公开(公告)日：2009-01-08

申请号：US12278768

申请日：2006-09-12

Applicant: Hyun Sik Kim ,  Myoung Choul Choi ,  Jong Shin Yoo

Inventor： Hyun Sik Kim ,  Myoung Choul Choi ,  Jong Shin Yoo

IPC: B01D59/44

CPC classification number: G01N27/62

Abstract: A tandem Fourier transform ion cyclotron resonance mass spectrometer is provided. In the mass spectrometer, the ions selected by a FT-ICR mass analyzer, which can perform an ion selection process and a mass measurement process with a time interval between the processes, are transmitted through an ion guide to a collision cell, which is located a predetermined distance from the FT-ICR mass analyzer, to split into fragment ions. The fragment ions are transmitted to the FT-ICR mass analyzer that measures the mass of the fragment ions. The fragment ions are generated in the collision cell 60 established separately from the FT-ICR mass analyzer 40 according to the mass spectrometer. Accordingly, It can solve various problems (e.g., the radius reduction of cyclotron motion of colliding ions, or the removal of periphery gas after generating the fragment ions) occurred in a tandem mass spectrometer using a conventional tandem-in-time mass analysis method. Also, a high resolution and with sensitivity measurement can be achieved. Moreover, when a reagent gas instead of a collision gas in the collision cell is injected, the gas phase reaction of the selected ions and the reagent gas can be observed, and the mass of the ions generated in the gas phase reaction can be measured.

Abstract translation: 提供了一种串联傅里叶变换离子回旋共振质谱仪。 在质谱仪中，可以进行离子选择处理的FT-ICR质量分析器选择的离子和在处理之间的时间间隔的质量测量处理通过离子导向器传输到位于 距离FT-ICR质量分析仪预定的距离，分裂为碎片离子。 碎片离子传输到测量碎片离子质量的FT-ICR质量分析仪。 在根据质谱仪与FT-ICR质量分析器40分开设置的碰撞单元60中产生碎片离子。 因此，可以使用常规的串联质量分析方法来解决在串联质谱仪中发生的各种问题（例如，产生碰撞离子的回旋加速器运动的半径减小或产生碎片离子之后的外围气体的去除）。 此外，可以实现高分辨率和灵敏度测量。 此外，当注入反应气体代替碰撞气体中的碰撞气体时，可以观察到所选择的离子和反应气体的气相反应，并且可以测量在气相反应中产生的离子的质量。

公开(公告)号：US07339378B2

公开(公告)日：2008-03-04

申请号：US11510233

申请日：2006-08-25

Applicant: Oc Hee Han ,  Kee Sung Han

Inventor： Oc Hee Han ,  Kee Sung Han

IPC: G01V3/00

CPC classification number: G01R33/34046 ,  G01R33/30 ,  G01R33/307

Abstract: Disclosed is a probe unit for nuclear magnetic resonance. The probe unit includes a cylindrical outer conductor, a cylindrical central conductor concentrically disposed in the outer conductor, a membrane assembly and fluid paths guiding the flow of fluid in the space between the central and outer conductors. By applying a RF current between the outer and central conductors, a sensing magnetic field is generated in a radial direction. Accordingly, it is possible to detect in real time and in situ, variations of characteristics as well as spatial distribution changes, of a fluid under reaction and/or passing through the fluid paths in the space between the central and outer conductors.

Abstract translation: 公开了一种用于核磁共振的探针单元。 探针单元包括圆柱形外部导体，同心地设置在外部导体中的圆柱形中心导体，膜组件和引导中央和外部导体之间的空间中的流体流的流体路径。 通过在外导体和中心导体之间施加RF电流，沿径向方向产生感测磁场。 因此，可以实时地和现场地检测反应中的流体的特性和空间分布变化的变化和/或通过中心和外​​部导体之间的空间中的流体路径。

公开(公告)号：US20070185316A1

公开(公告)日：2007-08-09

申请号：US11307457

申请日：2006-02-08

Applicant: Yeong Hee AHN ,  Jong Shin YOO ,  Soohyun KIM

Inventor： Yeong Hee AHN ,  Jong Shin YOO ,  Soohyun KIM

IPC: C07K14/47 ,  C07H5/06

CPC classification number: C07H5/06

Abstract: The present invention relates to the method for tagging of carbohydrates with active methylene compound. Particularly, it relates to the method for tagging of carbohydrates with active methylene compound comprising the step of preparing carbohydrate conjugate in which carbohydrate and methylene compound are combined by mixing carbohydrate mixture and methylene compound under aqueous polar aprotic solvent containing amine base catalyst. The tagging method of the present invention does not need many kinds of chemical reagent and the reactions can be taken even in the presence of certain amount of impurities. So, it can be used for the analysis of oligosaccharide present in the various kinds of samples.

Abstract translation: 本发明涉及用活性亚甲基化合物标记碳水化合物的方法。 特别涉及用活性亚甲基化合物标记碳水化合物的方法，包括制备碳水化合物缀合物的步骤，其中碳水化合物和亚甲基化合物通过混合碳水化合物混合物和亚甲基化合物在含有胺基催化剂的极性非质子溶剂的水溶液下混合。 本发明的标记方法不需要多种化学试剂，即使在存在一定量的杂质的情况下也可以进行反应。 因此，可用于各种样品中存在的寡糖的分析。

公开(公告)号：US07250266B2

公开(公告)日：2007-07-31

申请号：US10907945

申请日：2005-04-21

Applicant: Yeong Hee Ahn ,  Jong Shin Yoo ,  Jin Young Kim ,  Kun Cho

Inventor： Yeong Hee Ahn ,  Jong Shin Yoo ,  Jin Young Kim ,  Kun Cho

IPC: G01N33/00

CPC classification number: G01N33/6848 ,  A61K31/155 ,  G01N33/6803 ,  G01N33/6842 ,  G01N2458/15

Abstract: Disclosed is a method of analyzing mass of the phosphoproteins or phosphopeptides and of analyzing phosphorylated positions at a phosphoprotein or phosphopeptide, comprising the steps of: 1) dephosphorylating at least one Ser and/or Thr residue of the phosphoprotein or phosphopeptide; 2) tagging the dephosphorylated amino acid residues with a tag having a R-L-G moiety wherein R is a nucleophilic functional group that selectively bind with dephosphorylated amino acid residues, G is selected from the group consisting of guanidine moiety or protected guanidine moiety such as a mono-N-protected guanidino group, a di-N,N′-protected guanidino group and an N′-protected guanidino group, and L is a linker linking the R and the G; and 3) subjecting the tagged proteins or peptides to mass spectrometry. The method is capable of precisely analyzing mass of phosphoproteins of trace amounts as well as positions of phosphoryated amino acids.

Abstract translation: 公开了一种分析磷蛋白或磷酸肽的质量并分析磷蛋白或磷酸肽的磷酸化位置的方法，包括以下步骤：1）使磷酸蛋白或磷酸肽的至少一个Ser和/或Thr残基去磷酸化; 2）用具有RLG部分的标签标记去磷酸化的氨基酸残基，其中R是与去磷酸化的氨基酸残基选择性结合的亲核官能团，G选自胍部分或被保护的胍部分，例如单 - N-保护的胍基，二-N，N'保护的胍基和N'保护的胍基，L是连接R和G的连接基团; 和3）对标记的蛋白质或肽进行质谱分析。 该方法能够精确分析痕量的磷蛋白质量以及磷酸化氨基酸的位置。

公开(公告)号：US20040191872A1

公开(公告)日：2004-09-30

申请号：US10438992

申请日：2003-05-16

Applicant: Korea Basic Science Institute

Inventor： Chaejoon Cheong ,  Chulhyun Lee ,  Hae-Kap Cheong ,  Jun-Soek Yoo ,  Eunha Hwang

IPC: C12P019/34 ,  C07H021/02

CPC classification number: C12P19/34

Abstract: A process for preparation of RNA using an affinity column and a DNAzyme is disclosed. By the process, a target RNA can be mass-produced with a high yield, a high resolution and a high purification efficiency. The process includes the steps of preparing a RNA adduct including a target RNA and a tail RNA sequence which is affixed to the 3null-terminal of the target RNA and can complementary combine with an oligo-dN sequence of an oligo-dN affinity column; purifying the RNA adduct by combining the RNA adduct to the oligo-dN affinity column; and obtaining the target RNA by cleaving the purified RNA adduct with a DNAzyme. Preferably, the process for preparation of RNA further includes the steps of removing a RNA fragment cleaved by the DNAzyme by using an affinity column; and decomposing the DNAzyme with a DNA nuclease, and then removing decomposed DNAzyme fragments with a gel filtration column.

Abstract translation: 公开了使用亲和柱和DNAzyme制备RNA的方法。 通过该方法，可以高产率，高分辨率和高纯化效率大量生产靶RNA。 该方法包括制备包含目标RNA和尾RNA序列的RNA加合物的步骤，所述RNA加合物附着于靶RNA的3'末端，并可与oligo-dN亲和柱的oligo-dN序列互补结合; 通过将RNA加合物与oligo-dN亲和柱结合来纯化RNA加合物; 并通过用DNA酶切割纯化的RNA加合物来获得靶RNA。 优选地，RNA的制备方法还包括通过使用亲和柱除去DNA酶切割的RNA片段的步骤; 并用DNA核酸酶分解DNA酶，然后用凝胶过滤柱除去分解的DNA酶片段。