公开(公告)号：US20240363321A1

公开(公告)日：2024-10-31

申请号：US18768828

申请日：2024-07-10

Applicant: Micromass UK Limited

Inventor： Keith Richardson ,  Jason Lee Wildgoose

IPC: H01J49/00 ,  H01J49/02

CPC classification number: H01J49/0031 ,  H01J49/0009 ,  H01J49/004 ,  H01J49/005 ,  H01J49/0072 ,  H01J49/025

Abstract: A method of mass spectrometry is disclosed comprising: performing a plurality of cycles of operation during a single experimental run, wherein each cycle comprises: mass selectively transmitting precursor ions of a single mass, or range of masses, through or out of a mass separator or mass filter at any given time, wherein the mass separator or mass filter is operated such that the single mass or range of masses transmitted therefrom is varied with time; and mass analysing ions.

公开(公告)号：US20240329052A1

公开(公告)日：2024-10-03

申请号：US18737836

申请日：2024-06-07

Applicant: Protein Metrics, LLC

Inventor： Andrew NICHOLS ,  Marshall BERN ,  Yong Joo KIL ,  Eric Carlson

IPC: G01N33/68 ,  H01J49/00

CPC classification number: G01N33/6848 ,  H01J49/0036 ,  H01J49/004

Abstract: Methods and apparatuses for the identification and/or characterization of properties of a macromolecule based on mass spectrometry data. Specifically, described herein are methods and apparatuses for converting peptide-level data into a pseudo-intact mass spectra. Also described herein are methods and apparatuses for converting peptide-level data into a pseudo-electropherogram. The methods may be well suited for analyzing proteins and protein complexes, including estimating properties of post-translational modifications of the proteins and protein complexes. Methods may include generating a theoretical graph or spectrum based on peptide-level mass spectrometry data. In some embodiments, the theoretical graph may be a theoretical intact mass spectrum or a theoretical charge distribution spectrum.

公开(公告)号：US12087564B2

公开(公告)日：2024-09-10

申请号：US17557879

申请日：2021-12-21

Applicant: The Administrators of the Tulane Educational Fund

Inventor： Scott Grayson

IPC: H01J49/00 ,  C07D319/06 ,  C07D493/22

CPC classification number: H01J49/0009 ,  C07D319/06 ,  C07D493/22 ,  H01J49/004 ,  Y10T436/10

Abstract: Provided are synthetic dendrimer calibrants for mass spectrometry. The calibrants are distinguished by their relative case and rapidity of synthesis, comparatively low cost, long shelf life, high purity, and amenability to batch synthesis as mixtures. The latter characteristic enables parallel preparation of higher molecular weight compounds displaying useful distributions of discrete molecular weights, thereby providing multi-point mass spectrometry calibration standards. Methods of making, tuning and using said calibrants are provided.

公开(公告)号：US12080534B2

公开(公告)日：2024-09-03

申请号：US17869016

申请日：2022-07-20

Applicant: Micromass UK Limited

Inventor： Jason Lee Wildgoose ,  Keith Richardson ,  David J. Langridge ,  Martin Raymond Green ,  Steven Derek Pringle

IPC: H01J49/00 ,  G01N27/62 ,  G01N27/623 ,  H01J49/42

CPC classification number: H01J49/004 ,  G01N27/62 ,  G01N27/623 ,  H01J49/4235 ,  H01J49/427

Abstract: A method is disclosed comprising: trapping ions in an ion trap (40); applying a first force on the ions within the ion trap in a first direction, said force having a magnitude that is dependent upon the value of a physicochemical property of the ions; applying a second force on these ions in the opposite direction so that the ions separate according to the physicochemical property value as a result of the first and second forces; and then pulsing or driving ions out of one or more regions of the ion trap.

公开(公告)号：US20240186130A1

公开(公告)日：2024-06-06

申请号：US18554840

申请日：2022-04-14

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Bradley B. Schneider ,  Leigh Bedford

IPC: H01J49/00 ,  H01J49/04

CPC classification number: H01J49/0031 ,  H01J49/004 ,  H01J49/0431

Abstract: Systems and methods are disclosed for automated method parameter configuration for differential mobility separations. As non-limiting examples, various aspects of this disclosure provide receiving a sample in an open port interface; transferring the sample to an ionization source; ionizing the transferred sample; introducing the ionized sample into a mass spectrometer; mass analyzing the ionized sample to produce an initial mass analysis result; determining a peak width of the initial mass analysis result; and determining a dwell time for subsequent measurements based on the determined peak width, a pre-defined number of data points across subsequent mass analysis peak widths, and a number of different analytes to be assessed for the sample. The sample may be diluted and transferred to the ionization source by a sample introduction apparatus selected from a group including an acoustic droplet ejector (ADE), a pneumatic ejector, a piezoelectric ejector, and a hydraulic ejector.

公开(公告)号：US11988652B2

公开(公告)日：2024-05-21

申请号：US16951909

申请日：2020-11-18

Applicant: Thermo Finnigan LLC

Inventor： Joshua A. Silveira ,  Dale Lindseth ,  Gary A. Schultz ,  Eloy R. Wouters ,  Michael L. Poltash ,  Brandon Howard Robson

IPC: G01N30/72 ,  G01N30/14 ,  G01N30/60 ,  G01N33/68 ,  H01J49/00 ,  H01J49/04 ,  H01J49/16 ,  G01N30/02 ,  G01N30/04

CPC classification number: G01N30/7233 ,  G01N30/14 ,  G01N30/60 ,  G01N33/6848 ,  H01J49/0031 ,  H01J49/004 ,  H01J49/0436 ,  H01J49/165 ,  G01N2030/027

Abstract: Packed-tip electrospray ionization (ESI) emitters for mass spectrometry are described. In one aspect an ESI emitter stores a first type of particle. A liquid chromatography (LC) column is coupled with the emitter via a junction. The LC column stores a different type of particle than the ESI emitter to facilitate better chromatographic and ESI performance.

公开(公告)号：US11933770B2

公开(公告)日：2024-03-19

申请号：US18052003

申请日：2022-11-02

Applicant: Thermo Finnigan LLC

Inventor： Philip M. Remes ,  David A. Sarracino ,  Sebastien F. Gallien

IPC: G01N30/00 ,  G01N30/72 ,  G01N30/86 ,  G01N33/68 ,  H01J49/00 ,  H01J49/42

CPC classification number: G01N30/7233 ,  G01N30/8637 ,  G01N30/8641 ,  G01N30/8675 ,  G01N33/6848 ,  H01J49/004 ,  H01J49/4265 ,  G01N2458/15 ,  G01N2570/00

Abstract: A Liquid Chromatography Mass Spectrometry system comprises: a chromatograph; a mass spectrometer configured to ionize separated fractions of a sample received from the chromatograph; and a programmable processor operable to repeatedly execute the steps of: (i) causing the mass spectrometer to perform a data-independent analysis of the precursor ion species using a mass analyzer of the mass spectrometer; (ii) calculating one or more degree-of-matching scores that relate to detection of an internal standard; and (iii) if each of the degree-of-matching scores meets a respective degree-of-matching condition, performing a quantitative tandem mass spectrometric analyses of both the internal standard and the analyte; the programmable processor further operable to calculate a quantity of the analyte in the sample by comparison between intensities of one or more mass spectral signals generated by the quantitative tandem mass spectrometric analyses of the analyte and the internal standard.

公开(公告)号：US20240027397A1

公开(公告)日：2024-01-25

申请号：US18021759

申请日：2021-07-30

Applicant: MOBILion Systems, Inc.

Inventor： John Daniel DeBord ,  Liulin Deng ,  Zongyuan Chen

IPC: G01N27/623 ,  H01J49/00

CPC classification number: G01N27/623 ,  H01J49/004

Abstract: A system for capturing full resolution ion mobility data includes an ion mobility separation device that receives ions, guides a portion thereof, and separates the guided ions based on mobility. An ion detector receives the ions, detects ions having a predefined mass-to-charge ratio during a time period, and generates a responsive signal(s). A data acquisition system receives the signal(s) from the detector and generates a time dependent ion signal representing intensity of the signal(s) at different arrival time periods during the time period. A system for performing multi-analyte targeted data acquisition includes an ion mobility separation device that receives a stream of ions, guides a portion of the ions, and separates the ions based on mobility. An ion detector receives the ions and switches from detecting a first mass-to-charge ratio during a first arrival time to detecting a second mass-to-charge ratio during a second arrival time of a first scan.

公开(公告)号：US11835499B2

公开(公告)日：2023-12-05

申请号：US16944640

申请日：2020-07-31

Applicant: CITY OF HOPE

Inventor： Timothy W. Synold ,  John Termini ,  Sarah Shuck

IPC: G01N30/72 ,  G01N1/28 ,  G01N33/483 ,  G01N33/49 ,  G01N33/493 ,  H01J49/00 ,  G01N30/02

CPC classification number: G01N30/7266 ,  G01N1/28 ,  G01N33/4833 ,  G01N33/49 ,  G01N33/493 ,  H01J49/004 ,  G01N2001/2893 ,  G01N2030/027

Abstract: Methods of quantifying a N2-(1-carboxyethyl)-2′-deoxyguanosine (CEdG) and N2-(1-carboxyethyl)-guanosine (CEG) levels in biological samples and comparing those levels to known normal levels can diagnose a number of metabolic disorders or complications associated therewith, including diabetes, its associated complications, and cancer. Methods can also determine whether therapies for disorders are effective by measuring CEdG and CEG levels before and after treatment. Measurement of CEdG and CEG levels is achieved by using liquid chromatography electrospray ionization tandem mass spectrometry.

公开(公告)号：US11798794B2

公开(公告)日：2023-10-24

申请号：US16996108

申请日：2020-08-18

Applicant: Quest Diagnostics Investments Incorporated

Inventor： Qibo Jiang ,  Sum Chan ,  Richard E. Reitz

IPC: G01N33/82 ,  G01N33/49 ,  H01J49/00

CPC classification number: H01J49/0031 ,  G01N33/49 ,  G01N33/82 ,  H01J49/004 ,  Y10T436/145555

Abstract: Provided are methods of detecting the presence or amount of the active form of vitamin B6, pyridoxal 5′-phosphate, in a body fluid sample using tandem mass spectrometry coupled with liquid chromatography.