公开(公告)号：US6015715A

公开(公告)日：2000-01-18

申请号：US952506

申请日：1998-01-06

Applicant: Uwe Kirschner ,  Matthias Lau ,  Birgit Hannemann ,  Adriana Tamachkiarowa

Inventor： Uwe Kirschner ,  Matthias Lau ,  Birgit Hannemann ,  Adriana Tamachkiarowa

IPC: G01N31/22 ,  G01N21/78 ,  G01N21/64 ,  G01N33/48

CPC classification number: G01N21/783

Abstract: Method of manufacturing a sensitive single-layer system for optically detecting a concentration of an analyte, such as, for example, oxygen, the analyte being capable of investigation in liquid or gaseous aggregate condition or in a dissolved condition, by adsorption, luminescence or luminescence quenching of an indicator sensitive to the analyte, which is preferably permanently immobilized in the optical beam path. The sensitive single-layer system achieves a reproducible and extremely short response behavior. Indicators are adsorbed on a filler material and thereafter a mixture is produced with a matrix material permeable to the analyte to be detected. Then, under the action of pressure, the mixture is extruded onto a substrate, the layer thickness being formed in dependence on the applied pressure used. The applied sensitive layer is polymerized, polycondensed or hardened and the layer is also homogenized by swelling in a fluorescence indicator solution.

Abstract translation: PCT No.PCT / DE96 / 00915 Sec。 371日期：1998年1月6日 102（e）日期1998年1月6日PCT提交1996年5月24日PCT公布。 公开号WO96 / 37768 日期1996年11月28日制造用于光学检测分析物（例如氧）浓度的敏感单层系统的方法，所述分析物能够在液体或气体聚集条件或溶解状态下通过 对分析物敏感的指示剂的吸附，发光或发光猝灭，其优选地永久地固定在光束路径中。 敏感的单层系统实现了可重现和极短的响应行为。 指示剂被吸附在填充材料上，然后用可检测待分析物的基质材料生产混合物。 然后，在压力作用下，将混合物挤压到基底上，根据所使用的施加压力形成层厚度。 所应用的敏感层被聚合，缩聚或硬化，并且层也通过在荧光指示剂溶液中溶胀而均化。