公开(公告)号：US06538735B1

公开(公告)日：2003-03-25

申请号：US09512707

申请日：2000-02-25

Applicant: Juerg Duebendorfer ,  Donald Jones ,  Kenneth Neumann ,  Chang Jin Wang

Inventor： Juerg Duebendorfer ,  Donald Jones ,  Kenneth Neumann ,  Chang Jin Wang

IPC: G01J330

CPC classification number: G01N21/6452 ,  G01N21/6428 ,  G01N2201/0648

Abstract: An apparatus for measuring light in samples using a high intensity light source, is presented. The system utilizes bifurcated fiber bundles to transmit light at the excitation and emission wavelength bands. It also uses a band-pass filter for eliminating extraneous light, including that which corresponds to the excitation wavelength range, while permitting the emitted light to pass to a detector for quantitation. The system employs a shutter to shield the detector while the laser light source is activated, and a controller to intermittently activate the laser light and close the shutter. The apparatus preferably includes lenses for better illumination and read out conditions. The apparatus is employed in Luminescence Oxygen Channeling Immunoassays. The method has high sensitivity, accuracy and precision, and the apparatus is highly compact. Accordingly, the analyzer can perform assays in nanoliter to microliter sample volumes in standard microplates having at least 96, 384 or 1536 wells.

Abstract translation: 提出了一种使用高强度光源测量样品中的光的装置。 该系统利用分叉光纤束在激发和发射波长带发射光。 它还使用带通滤波器来消除外来光，包括对应于激发波长范围的光，同时允许发射的光通过检测器进行定量。 该系统在激活激光光源时使用快门屏蔽检测器，并且控制器间歇地激活激光并关闭快门。 该装置优选地包括用于更好照明和读出条件的透镜。 该装置用于发光氧通道免疫测定。 该方法灵敏度高，精度高，精度高，装置高度紧凑。 因此，分析仪可以在具有至少96个，384个或1536个孔的标准微孔板中进行纳升至微升样品体积的测定。