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公开(公告)号:US09932554B2
公开(公告)日:2018-04-03
申请号:US13395107
申请日:2010-09-17
Applicant: Chung-Soon Im , Jane Kim
Inventor: Chung-Soon Im , Jane Kim
CPC classification number: C12M31/02 , A01G33/00 , C12M21/02 , C12M21/12 , C12M31/08 , C12M31/10 , C12N1/12 , C12P7/6463 , C12P7/649 , Y02E50/13 , Y02T50/678
Abstract: Bioreactors and methods for cultivating microalgae are provided herein. The bioreactor and methods include features and modifications to improve heterotrophic growth efficiency by providing a light signal.
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公开(公告)号:US08889402B2
公开(公告)日:2014-11-18
申请号:US13273179
申请日:2011-10-13
Applicant: Donald E. Trimbur , Chung-Soon Im , Harrison F. Dillon , Anthony G. Day , Scott Franklin , Anna Coragliotti
Inventor: Donald E. Trimbur , Chung-Soon Im , Harrison F. Dillon , Anthony G. Day , Scott Franklin , Anna Coragliotti
IPC: C12P7/64 , C12N1/12 , C11C3/00 , C10L1/02 , C12N1/32 , C12N9/04 , C12N9/02 , C12N9/12 , C12N9/16 , C12N9/20 , C12N9/26 , C12N9/88 , C12P5/02
CPC classification number: C10L1/026 , C07H21/04 , C10L1/06 , C10L2200/0484 , C10L2270/026 , C10L2270/04 , C11C3/00 , C12N1/12 , C12N1/32 , C12N9/0006 , C12N9/0008 , C12N9/1205 , C12N9/14 , C12N9/16 , C12N9/20 , C12N9/2408 , C12N9/2431 , C12N9/88 , C12N15/79 , C12P5/02 , C12P7/6409 , C12P7/6436 , C12P7/6445 , C12P7/6463 , C12P7/649 , C12P21/06 , C12Y302/01026 , Y02E50/10 , Y02E50/13 , Y02E50/343 , Y02P20/52 , Y02T50/678
Abstract: The invention provides methods of cultivating oil-bearing microbes using xylose alone or in combination with other depolymerized cellulosic material. Also provided are microorganisms comprising an exogenous gene encoding a polysaccharide degrading enzyme, such as a cellulase, a hemicellulase, a pectinase, or a driselase. Some methods of microbial fermentation are provided that comprise the use of xylose and depolymerized cellulosic materials for the production of oil-bearing microorgansims.
Abstract translation: 本发明提供了使用单独的木糖或与其它解聚的纤维素材料组合培养含油微生物的方法。 还提供了包含编码多糖降解酶的外源基因如纤维素酶,半纤维素酶,果胶酶或者碎裂酶的微生物。 提供了微生物发酵的一些方法,其包括使用木糖和解聚的纤维素材料来生产含油微生物。
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3.
公开(公告)号:US08647397B2
公开(公告)日:2014-02-11
申请号:US13558252
申请日:2012-07-25
Applicant: Donald E. Trimbur , Chung-Soon Im , Harrison F. Dillon , Anthony G. Day , Scott Franklin , Anna Coragliotti
Inventor: Donald E. Trimbur , Chung-Soon Im , Harrison F. Dillon , Anthony G. Day , Scott Franklin , Anna Coragliotti
CPC classification number: C10L1/026 , C07H21/04 , C10L1/06 , C10L2200/0484 , C10L2270/026 , C10L2270/04 , C11C3/00 , C12N1/12 , C12N1/32 , C12N9/0006 , C12N9/0008 , C12N9/1205 , C12N9/14 , C12N9/16 , C12N9/20 , C12N9/2408 , C12N9/2431 , C12N9/88 , C12N15/79 , C12P5/02 , C12P7/6409 , C12P7/6436 , C12P7/6445 , C12P7/6463 , C12P7/649 , C12P21/06 , C12Y302/01026 , Y02E50/10 , Y02E50/13 , Y02E50/343 , Y02P20/52 , Y02T50/678
Abstract: The invention provides methods of modifying the lipids produced by microbial organisms through genetic engineering. The invention also provides genetically engineered microbes and methods of fermenting microbes for oil production. Also provided are oils, fuels, oleochemicals, chemical precursors, and other compounds manufactured by such modified microorganisms. Exemplary oil-bearing organisms include organisms containing one or more exogenous genes encoding a fatty acyl-ACP thioesterase, fatty acyl-CoA/aldehyde reductase, fatty acyl-CoA reductase, fatty aldehyde reductase, fatty aldehyde decarbonylase, and/or an acyl carrier protein.
Abstract translation: 本发明提供了通过遗传工程修饰由微生物生物产生的脂质的方法。 本发明还提供基因工程微生物和发酵用于生产油的微生物的方法。 还提供了由这些改性微生物制造的油,燃料,油化学品,化学前体和其它化合物。 示例性含油生物包括含有一种或多种编码脂肪酰基-ACP硫酯酶,脂肪酰辅酶A /醛还原酶,脂肪酰辅酶A还原酶,脂肪醛还原酶,脂肪醛脱羰酶和/或酰基载体蛋白的外源基因的生物体 。
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公开(公告)号:US20110015417A1
公开(公告)日:2011-01-20
申请号:US12772173
申请日:2010-04-30
Applicant: Donald E. Trimbur , Chung-Soon Im , Harrison F. Dillon , Anthony G. Day , Scott Franklin , Anna Coragliotti
Inventor: Donald E. Trimbur , Chung-Soon Im , Harrison F. Dillon , Anthony G. Day , Scott Franklin , Anna Coragliotti
CPC classification number: C10L1/026 , C07H21/04 , C10L1/06 , C10L2200/0484 , C10L2270/026 , C10L2270/04 , C11C3/00 , C12N1/12 , C12N1/32 , C12N9/0006 , C12N9/0008 , C12N9/1205 , C12N9/14 , C12N9/16 , C12N9/20 , C12N9/2408 , C12N9/2431 , C12N9/88 , C12N15/79 , C12P5/02 , C12P7/6409 , C12P7/6436 , C12P7/6445 , C12P7/6463 , C12P7/649 , C12P21/06 , C12Y302/01026 , Y02E50/10 , Y02E50/13 , Y02E50/343 , Y02P20/52 , Y02T50/678
Abstract: The invention provides methods of cultivating oil-bearing microbes using xylose alone or in combination with other depolymerized cellulosic material. Also provided are microorganisms comprising an exogenous gene encoding a polysaccharide degrading enzyme, such as a cellulase, a hemicellulase, a pectinase, or a driselase. Some methods of microbial fermentation are provided that comprise the use of xylose and depolymerized cellulosic materials for the production of oil-bearing microorgansims.
Abstract translation: 本发明提供了使用单独的木糖或与其它解聚的纤维素材料组合培养含油微生物的方法。 还提供了包含编码多糖降解酶的外源基因如纤维素酶,半纤维素酶,果胶酶或者碎裂酶的微生物。 提供了微生物发酵的一些方法,其包括使用木糖和解聚的纤维素材料来生产含油微生物。
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公开(公告)号:US20110014665A1
公开(公告)日:2011-01-20
申请号:US12772170
申请日:2010-04-30
Applicant: Donald E. Trimbur , Chung-Soon Im , Harrison F. Dillon , Anthony G. Day , Scott Franklin , Anna Coragliotti
Inventor: Donald E. Trimbur , Chung-Soon Im , Harrison F. Dillon , Anthony G. Day , Scott Franklin , Anna Coragliotti
CPC classification number: C10L1/026 , C07H21/04 , C10L1/06 , C10L2200/0484 , C10L2270/026 , C10L2270/04 , C11C3/00 , C12N1/12 , C12N1/32 , C12N9/0006 , C12N9/0008 , C12N9/1205 , C12N9/14 , C12N9/16 , C12N9/20 , C12N9/2408 , C12N9/2431 , C12N9/88 , C12N15/79 , C12P5/02 , C12P7/6409 , C12P7/6436 , C12P7/6445 , C12P7/6463 , C12P7/649 , C12P21/06 , C12Y302/01026 , Y02E50/10 , Y02E50/13 , Y02E50/343 , Y02P20/52 , Y02T50/678
Abstract: The invention provides methods of cultivating oil-bearing microbes using cellulosic material. Also provided are microorganisms containing one or more exogenous genes that facilitate the use of cellulosic materials as a feedstock. Also provided are microorganisms and methods for manufacturing non-alcohol-based fuels and fuel feedstocks through a process of converting cellulosic materials into oils.
Abstract translation: 本发明提供使用纤维素材料培养含油微生物的方法。 还提供了含有一种或多种促进使用纤维素材料作为原料的外源基因的微生物。 还提供了通过将纤维素材料转化为油的方法来制造非醇类燃料和燃料原料的微生物和方法。
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公开(公告)号:US20120028319A1
公开(公告)日:2012-02-02
申请号:US13273179
申请日:2011-10-13
Applicant: Donald E. Trimbur , Chung-Soon Im , Harrison F. Dillon , Anthony G. Day , Scott Franklin , Anna Coragliotti
Inventor: Donald E. Trimbur , Chung-Soon Im , Harrison F. Dillon , Anthony G. Day , Scott Franklin , Anna Coragliotti
CPC classification number: C10L1/026 , C07H21/04 , C10L1/06 , C10L2200/0484 , C10L2270/026 , C10L2270/04 , C11C3/00 , C12N1/12 , C12N1/32 , C12N9/0006 , C12N9/0008 , C12N9/1205 , C12N9/14 , C12N9/16 , C12N9/20 , C12N9/2408 , C12N9/2431 , C12N9/88 , C12N15/79 , C12P5/02 , C12P7/6409 , C12P7/6436 , C12P7/6445 , C12P7/6463 , C12P7/649 , C12P21/06 , C12Y302/01026 , Y02E50/10 , Y02E50/13 , Y02E50/343 , Y02P20/52 , Y02T50/678
Abstract: The invention provides methods of cultivating oil-bearing microbes using xylose alone or in combination with other depolymerized cellulosic material. Also provided are microorganisms comprising an exogenous gene encoding a polysaccharide degrading enzyme, such as a cellulase, a hemicellulase, a pectinase, or a driselase. Some methods of microbial fermentation are provided that comprise the use of xylose and depolymerized cellulosic materials for the production of oil-bearing microorgansims.
Abstract translation: 本发明提供了使用单独的木糖或与其它解聚的纤维素材料组合培养含油微生物的方法。 还提供了包含编码多糖降解酶的外源基因如纤维素酶,半纤维素酶,果胶酶或者碎裂酶的微生物。 提供了微生物发酵的一些方法,其包括使用木糖和解聚的纤维素材料来生产含油微生物。
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公开(公告)号:US20110190522A1
公开(公告)日:2011-08-04
申请号:US13029061
申请日:2011-02-16
Applicant: Donald E. Trimbur , Chung-Soon Im , Harrison F. Dillon , Anthony G. Day , Scott Franklin , Anna Coragliotti
Inventor: Donald E. Trimbur , Chung-Soon Im , Harrison F. Dillon , Anthony G. Day , Scott Franklin , Anna Coragliotti
CPC classification number: C10L1/026 , C07H21/04 , C10L1/06 , C10L2200/0484 , C10L2270/026 , C10L2270/04 , C11C3/00 , C12N1/12 , C12N1/32 , C12N9/0006 , C12N9/0008 , C12N9/1205 , C12N9/14 , C12N9/16 , C12N9/20 , C12N9/2408 , C12N9/2431 , C12N9/88 , C12N15/79 , C12P5/02 , C12P7/6409 , C12P7/6436 , C12P7/6445 , C12P7/6463 , C12P7/649 , C12P21/06 , C12Y302/01026 , Y02E50/10 , Y02E50/13 , Y02E50/343 , Y02P20/52 , Y02T50/678
Abstract: The invention provides methods of manufacturing alkanes from triglyceride oils produced through fermentation of oil-bearing microbes. The processes provided herein can utilize a variety of carbohydrate feedstocks including cane bagasse, sugar beet pulp, corn stover, glycerol, corn starch, sorghum, molasses, waste glycerol, and other renewable materials. These processes further comprise hydrotreating, hydrocracking, isomerization, distillation, and other petrochemical processes for use with oil-bearing microbes and products derived therefrom to manufacture fuels. Particular embodiments include the manufacture of ASTM D975 and ASTM D1655 compliant fuels. Genetically engineered microbes provided herein can be used in the manufacture of renewable diesel and renewable jet fuel.
Abstract translation: 本发明提供了通过含油微生物发酵产生的甘油三酯油制备烷烃的方法。 本文提供的方法可以利用多种碳水化合物原料,包括甘蔗渣,甜菜浆,玉米秸秆,甘油,玉米淀粉,高粱,糖蜜,废甘油和其他可再生材料。 这些方法还包括与含油微生物一起使用的加氢处理,加氢裂化,异构化,蒸馏和其它石油化学方法以及由其衍生的产物来制造燃料。 具体实施方案包括制造ASTM D975和符合ASTM D1655的燃料。 本文提供的基因工程微生物可用于制造可再生柴油和可再生喷气燃料。
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公开(公告)号:US10479969B2
公开(公告)日:2019-11-19
申请号:US13270515
申请日:2011-10-11
Applicant: Jane Kim , Chung-Soon Im
Inventor: Jane Kim , Chung-Soon Im
Abstract: Bioreactors and methods for cultivating microalgae under heterotrophic growth conditions using fruit wastewater as a carbon source are provided herein. The heterotrophic growth conditions include low irradiance of light sufficient to initiate light-activated metabolism, but not photosynthesis. The methods provide a cost effective and environmentally friendly solution for converting wastewater from dried fruit and wine industries into products like biofuels.
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公开(公告)号:US08497116B2
公开(公告)日:2013-07-30
申请号:US12960388
申请日:2010-12-03
Applicant: Donald E. Trimbur , Chung-Soon Im , Harrison F. Dillon , Anthony G. Day , Scott Franklin , Anna Coragliotti
Inventor: Donald E. Trimbur , Chung-Soon Im , Harrison F. Dillon , Anthony G. Day , Scott Franklin , Anna Coragliotti
CPC classification number: C10L1/026 , C07H21/04 , C10L1/06 , C10L2200/0484 , C10L2270/026 , C10L2270/04 , C11C3/00 , C12N1/12 , C12N1/32 , C12N9/0006 , C12N9/0008 , C12N9/1205 , C12N9/14 , C12N9/16 , C12N9/20 , C12N9/2408 , C12N9/2431 , C12N9/88 , C12N15/79 , C12P5/02 , C12P7/6409 , C12P7/6436 , C12P7/6445 , C12P7/6463 , C12P7/649 , C12P21/06 , C12Y302/01026 , Y02E50/10 , Y02E50/13 , Y02E50/343 , Y02P20/52 , Y02T50/678
Abstract: The invention provides methods of manufacturing oils and oil-based products such as transportation fuels, industrial chemicals, lubricants and plastics using sugar cane, sugar cane juice, corn steep liquor, corn starch or depolymerized cellulosic material as a feedstock for bioproduction processes. Oils are manufactured using microalgae expressing sucrose invertase, which allows the microalgae to produce oil using sucrose-based feedstocks, along or in combination with other feedstocks.
Abstract translation: 本发明提供了使用甘蔗,甘蔗汁,玉米浆,玉米淀粉或解聚纤维素材料作为生物生产过程的原料制造油和油基产品如运输燃料,工业化学品,润滑剂和塑料的方法。 使用表达蔗糖转化酶的微藻制造油,其允许微藻使用基于蔗糖的原料,沿着或与其他原料组合生产油。
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10.
公开(公告)号:US08278090B1
公开(公告)日:2012-10-02
申请号:US12497257
申请日:2009-07-02
Applicant: Chung-Soon Im , Diana Vincent , Rika Regentin , Anna Coragliotti
Inventor: Chung-Soon Im , Diana Vincent , Rika Regentin , Anna Coragliotti
Abstract: The invention discloses novel methods of producing hydrocarbons through heterotrophic cultivation of Botryococcus braunii. Also provided are novel hydrocarbon compositions. A preferred species for engineering is the microalgae species Botryococcus braunii. Additional methods of cultivation include providing certain nutrient sources.
Abstract translation: 本发明公开了通过葡萄球菌的异养培养生产烃的新方法。 还提供了新的烃组合物。 优选的工程物种是微藻类Botryococcus braunii。 其他栽培方法包括提供某些营养源。
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