公开(公告)号：US20060127902A1

公开(公告)日：2006-06-15

申请号：US10524432

申请日：2003-08-15

Applicant: Stephen Madden ,  Clarence Cook ,  Brian Cook ,  John Laterra ,  Kevin Walter

Inventor： Stephen Madden ,  Clarence Cook ,  Brian Cook ,  John Laterra ,  Kevin Walter

IPC: C12Q1/68 ,  G01N33/567 ,  G01N33/53

CPC classification number: G01N33/57407 ,  C12N5/0693 ,  C12Q1/6886 ,  C12Q2600/112 ,  C12Q2600/136 ,  G01N33/57484 ,  G01N2500/00

Abstract: To gain a better understanding of brain tumor angiogenesis, new techniques for isolating brain endothelial cells (ECs) and evaluating gene expression patterns were developed. When transcripts from brain ECs derived from normal and malignant colorectal tissues were compared with transcripts from non-endothelial cells, genes predominantly expressed in the endothelium were identified. Comparison between normal- and tumor-derived endothelium revealed genes that were specifically elevated in tumor-associated brain endothelium. These results confirm that neoplastic and normal endothelium in human brains are distinct at the molecular level, and have significant implications for the development of anti-angiogenic therapies in the future.

Abstract translation: 为了更好地了解脑肿瘤血管生成，开发了分离脑内皮细胞（ECs）和评估基因表达模式的新技术。 将来自正常和恶性结肠直肠组织的脑EC的转录物与来自非内皮细胞的转录物进行比较，鉴定出在内皮中主要表达的基因。 正常和肿瘤来源的内皮的比较显示在肿瘤相关脑内皮细胞中特异性升高的基因。 这些结果证实，人脑中的肿瘤和正常内皮在分子水平上是不同的，并且对未来抗血管生成疗法的发展具有重要的意义。