公开(公告)号：US07615780B2

公开(公告)日：2009-11-10

申请号：US11427544

申请日：2006-06-29

Applicant: Steven Alfred Tysoe ,  Eugene Barash ,  Andrew David Pris

Inventor： Steven Alfred Tysoe ,  Eugene Barash ,  Andrew David Pris

IPC: H01L23/58

CPC classification number: H01S5/34333 ,  B82Y20/00 ,  H01S5/005 ,  H01S5/02248 ,  H01S5/18308 ,  H01S5/18338 ,  H01S5/18341

Abstract: Disclosed herein are biosensors and methods for making and using the same. In one embodiment, the sensor for detecting an analyte comprises: a substrate, recognition elements specific for the analyte, an excitation source, a detector, a chamber located between the substrate and the excitation source and between the substrate and the detector, and an emission filter. The recognition elements are tethered to the substrate such that the recognition elements can be exposed to a sample. The excitation source is capable of emitting a first light having a first light peak intensity at a first wavelength, wherein the first light can excite a luminophore to emit a second light when the recognition elements interact with the analyte. The detector is capable of detecting the second light emitted by the luminophore. The emission filter is capable of filtering in a band gap that includes the first light peak intensity.

Abstract translation: 本文公开了生物传感器及其制造和使用方法。 在一个实施例中，用于检测分析物的传感器包括：基底，对分析物特异的识别元件，激发源，检测器，位于基底和激发源之间以及基底和检测器之间的腔室，以及发射 过滤。 将识别元件束缚于基底，使得识别元件可以暴露于样品。 激发源能够发射具有第一波长的第一光峰强度的第一光，其中当识别元件与分析物相互作用时，第一光可以激发发光体以发射第二光。 检测器能够检测由发光体发出的第二光。 发射滤波器能够在包括第一光峰强度的带隙中进行滤波。

公开(公告)号：US20080280374A1

公开(公告)日：2008-11-13

申请号：US11745827

申请日：2007-05-08

Applicant: RADISLAV ALEXANDROVICH POTYRAILO ,  EUGENE BARASH ,  KATHERINE DOVIDENKO ,  PETER WILLIAM LORRAINE

Inventor： RADISLAV ALEXANDROVICH POTYRAILO ,  EUGENE BARASH ,  KATHERINE DOVIDENKO ,  PETER WILLIAM LORRAINE

IPC: G01N21/76

CPC classification number: G01N21/554 ,  G01N21/648 ,  G01N33/54373 ,  G01N33/553 ,  G01N2021/6439

Abstract: Methods and systems for detecting biological or biochemical analytes generally comprising, a metal film having one or more surfaces comprising one or more submicron structures; a device for applying one or more analytes to at least a portion of the film surface to interact with said metal film; a light source for illuminating a surface of the metal film so that at least some of the light is adapted to be optically altered by the functionalized metal film; and an optical detection subsystem for collecting the optically altered light, wherein the altered light is indicative of surface plasmon resonance on the film, and detecting one or more properties of the analytes based on the collected light.

Abstract translation: 用于检测生物或生化分析物的方法和系统通常包括具有一个或多个包含一个或多个亚微米结构的表面的金属膜; 用于将一种或多种分析物施加到所述膜表面的至少一部分以与所述金属膜相互作用的装置; 用于照射金属膜的表面的光源，使得至少一些光适于被官能化金属膜光学改变; 以及用于收集光学改变的光的光学检测子系统，其中改变的光指示膜上的表面等离子体共振，以及基于所收集的光检测分析物的一种或多种性质。

公开(公告)号：US20080003664A1

公开(公告)日：2008-01-03

申请号：US11427504

申请日：2006-06-29

Applicant: Steven Tysoe ,  Eugene Barash ,  Thomas Stecher

Inventor： Steven Tysoe ,  Eugene Barash ,  Thomas Stecher

IPC: H01J3/14 ,  C12M1/34

CPC classification number: G01N21/0303 ,  B01L3/5027

Abstract: A field deployable optical assembly for use in testing a light-responsive sample is disclosed. The assembly includes a microfluidic device, a first optical package, and a second optical package. The first optical package includes a light emitting diode (LED), a first optical device, and a first light-path control, the first optical package configured to guide and focus light from the LED onto the sample. The microfluidic device includes a tethered control substance. In response to a substance within the sample being associated with, and attaching to, the tethered control, the sample emits light. The second optical package includes a photo sensor, a second optical device, and a second light-path control, the second optical package configured to guide and focus the light emitted from the sample onto the photo sensor.

Abstract translation: 公开了用于测试光响应样品的现场部署光学组件。 组件包括微流体装置，第一光学封装和第二光学封装。 第一光学封装包括发光二极管（LED），第一光学器件和第一光路控制，第一光学封装构造成将来自LED的光引导并聚焦到样品上。 微流体装置包括栓系对照物质。 响应于样品中的物质与束缚对照相关联并连接到束带对照，样品发光。 第二光学封装包括光传感器，第二光学器件和第二光路控制，第二光学封装构造成将从样品发射的光引导并聚焦到光传感器上。

公开(公告)号：US20080230716A1

公开(公告)日：2008-09-25

申请号：US11427544

申请日：2006-06-29

Applicant: Steven Alfred Tysoe ,  Eugene Barash ,  Andrew David Pris

Inventor： Steven Alfred Tysoe ,  Eugene Barash ,  Andrew David Pris

IPC: G01N23/22

CPC classification number: H01S5/34333 ,  B82Y20/00 ,  H01S5/005 ,  H01S5/02248 ,  H01S5/18308 ,  H01S5/18338 ,  H01S5/18341

Abstract: Disclosed herein are biosensors and methods for making and using the same. In one embodiment, the sensor for detecting an analyte comprises: a substrate, recognition elements specific for the analyte, an excitation source, a detector, a chamber located between the substrate and the excitation source and between the substrate and the detector, and an emission filter. The recognition elements are tethered to the substrate such that the recognition elements can be exposed to a sample. The excitation source is capable of emitting a first light having a first light peak intensity at a first wavelength, wherein the first light can excite a luminophore to emit a second light when the recognition elements interact with the analyte. The detector is capable of detecting the second light emitted by the luminophore. The emission filter is capable of filtering in a band gap that includes the first light peak intensity.

Abstract translation: 本文公开了生物传感器及其制造和使用方法。 在一个实施例中，用于检测分析物的传感器包括：基底，对分析物特异的识别元件，激发源，检测器，位于基底和激发源之间以及基底和检测器之间的腔室，以及发射 过滤。 将识别元件束缚于基底，使得识别元件可以暴露于样品。 激发源能够发射具有第一波长的第一光峰强度的第一光，其中当识别元件与分析物相互作用时，第一光可以激发发光体以发射第二光。 检测器能够检测由发光体发出的第二光。 发射滤波器能够在包括第一光峰强度的带隙中进行滤波。

公开(公告)号：US20070206192A1

公开(公告)日：2007-09-06

申请号：US11363938

申请日：2006-03-01

Applicant: Pavel Fomitchov ,  Eugene Barash ,  Ahmad Yekta ,  Joseph Masino

Inventor： Pavel Fomitchov ,  Eugene Barash ,  Ahmad Yekta ,  Joseph Masino

IPC: G01N21/25

CPC classification number: G01N21/6458 ,  G01N21/47 ,  G01N2201/0407 ,  G01N2201/1042 ,  G02B21/0048 ,  G02B21/0076 ,  G02B21/367

Abstract: A system and method for multimode imaging of at least one sample is disclosed. The system includes at least one light source; an optical system selected responsive to a mode of operation of the imaging system; and a detector capable of selective reading of pixels. The at least one sample is moved elative to the optical system using a sample movement technique selected from the group consisting of step sample moving and continuous sample moving. The method includes the steps of (1) selecting a mode of operation for the imaging system; (2) transmitting light from at least one light source through an optical system selected in response to the mode of operation for the imaging system; (3) moving the at least one sample relative to the optical system using a sample movement technique selected from the group consisting of step sample moving and continuous sample moving; and (4) selectively reading pixels with a detector.

Abstract translation: 公开了一种用于至少一个样品的多模成像的系统和方法。 该系统包括至少一个光源; 响应于成像系统的操作模式选择的光学系统; 以及能够选择性地读取像素的检测器。 使用选自步骤样品移动和连续样品移动的样品移动技术将至少一个样品移动到光学系统。 该方法包括以下步骤：（1）选择成像系统的操作模式; （2）从至少一个光源透射通过响应于成像系统的操作模式选择的光学系统的光; （3）使用选自步骤样品移动和连续样品移动的样本移动技术来移动所述至少一个样品相对于所述光学系统; 和（4）用检测器选择性地读取像素。

公开(公告)号：US07567346B2

公开(公告)日：2009-07-28

申请号：US11363938

申请日：2006-03-01

Applicant: Pavel A. Fomitchov ,  Eugene Barash ,  Ahmad Yetka ,  Joseph Masino, III

Inventor： Pavel A. Fomitchov ,  Eugene Barash ,  Ahmad Yetka ,  Joseph Masino, III

IPC: G01N21/25

CPC classification number: G01N21/6458 ,  G01N21/47 ,  G01N2201/0407 ,  G01N2201/1042 ,  G02B21/0048 ,  G02B21/0076 ,  G02B21/367

Abstract: A system and method for multimode imaging of at least one sample is disclosed. The system includes at least one light source; an optical system selected responsive to a mode of operation of the imaging system; and a detector capable of selective reading of pixels. The at least one sample is moved elative to the optical system using a sample movement technique selected from the group consisting of step sample moving and continuous sample moving. The method includes the steps of (1) selecting a mode of operation for the imaging system; (2) transmitting light from at least one light source through an optical system selected in response to the mode of operation for the imaging system; (3) moving the at least one sample relative to the optical system using a sample movement technique selected from the group consisting of step sample moving and continuous sample moving; and (4) selectively reading pixels with a detector.

Abstract translation: 公开了一种用于至少一个样品的多模成像的系统和方法。 该系统包括至少一个光源; 响应于成像系统的操作模式选择的光学系统; 以及能够选择性地读取像素的检测器。 使用选自步骤样品移动和连续样品移动的样品移动技术将至少一个样品移动到光学系统。 该方法包括以下步骤：（1）选择成像系统的操作模式; （2）从至少一个光源透射通过响应于成像系统的操作模式选择的光学系统的光; （3）使用选自步骤样品移动和连续样品移动的样本移动技术来移动所述至少一个样品相对于所述光学系统; 和（4）用检测器选择性地读取像素。

公开(公告)号：US20090137058A1

公开(公告)日：2009-05-28

申请号：US11946342

申请日：2007-11-28

Applicant: Eugene Barash ,  Andrew David Pris

Inventor： Eugene Barash ,  Andrew David Pris

IPC: G01N21/64 ,  C12M1/34

CPC classification number: G01N21/645 ,  G01N21/0332 ,  G01N21/55 ,  G01N2021/575 ,  G01N2021/6471

Abstract: A device and method for detecting the presence of one or more analytes, bound directly or indirectly to a binding substrate functionalized with a fluorophore, based on measurements of fluorescence and reflectivity. The device and methods comprise an excitation source that emits light capable of being absorbed by a fluorophore and results in the fluorophore's excitation and emission, a fluorescent probe specific for the analyte that is attached via chemisorption to the binding substrate, a detector, and a processor adapted to determine the quantity of the one or more analytes present, by correlating measurements of reflected and fluorescent light.

Abstract translation: 基于荧光和反射率的测量来检测直接或间接结合到用荧光团官能化的结合底物的一种或多种分析物的存在的装置和方法。 该装置和方法包括发射能够被荧光团吸收并导致荧光团的激发和发射的光的激发源，对通过化学吸附附着到结合基底的分析物特异的荧光探针，检测器和处理器 适于通过将反射和荧光的测量相关联来确定存在的一种或多种分析物的量。

公开(公告)号：US07820428B2

公开(公告)日：2010-10-26

申请号：US11427504

申请日：2006-06-29

Applicant: Steven Tysoe ,  Eugene Barash ,  Thomas Stecher

Inventor： Steven Tysoe ,  Eugene Barash ,  Thomas Stecher

IPC: C12M1/34 ,  C12M3/00 ,  G01J1/10

CPC classification number: G01N21/0303 ,  B01L3/5027

Abstract: A field deployable optical assembly for use in testing a light-responsive sample is disclosed. The assembly includes a microfluidic device, a first optical package, and a second optical package. The first optical package includes a light emitting diode (LED), a first optical device, and a first light-path control, the first optical package configured to guide and focus light from the LED onto the sample. The microfluidic device includes a tethered control substance. In response to a substance within the sample being associated with, and attaching to, the tethered control, the sample emits light. The second optical package includes a photo sensor, a second optical device, and a second light-path control, the second optical package configured to guide and focus the light emitted from the sample onto the photo sensor.

Abstract translation: 公开了用于测试光响应样品的现场部署光学组件。 组件包括微流体装置，第一光学封装和第二光学封装。 第一光学封装包括发光二极管（LED），第一光学器件和第一光路控制，第一光学封装构造成将来自LED的光引导并聚焦到样品上。 微流体装置包括栓系对照物质。 响应于样品中的物质与束缚对照相关联并连接到束带对照，样品发光。 第二光学封装包括光传感器，第二光学器件和第二光路控制，第二光学封装构造成将从样品发射的光引导并聚焦到光传感器上。

公开(公告)号：US20100032582A1

公开(公告)日：2010-02-11

申请号：US12187815

申请日：2008-08-07

Applicant: Hua Xia ,  Li Zhu ,  Eugene Barash ,  Erin Jean Finehout ,  Matthew Damian Pietrzykowski

Inventor： Hua Xia ,  Li Zhu ,  Eugene Barash ,  Erin Jean Finehout ,  Matthew Damian Pietrzykowski

IPC: G01N21/64

CPC classification number: G01N21/645 ,  B01L3/5027 ,  G01J3/02 ,  G01J3/0208 ,  G01J3/0218 ,  G01J3/08 ,  G01J3/4406 ,  G01N21/6408 ,  G01N2021/6419 ,  G01N2021/6484 ,  G01N2201/0826 ,  G01N2201/0833

Abstract: A fluorescence detection system comprises a light source configured to produce an excitation light, an optical lens and a fiber bundle. The optical lens is configured to focus the excitation light to a sample to emit fluorescence and to collect the fluorescence. The fiber bundle probe comprises a transmitting fiber configured to transmit the excitation light to the optical lens, and a first receiving fiber configured to deliver the collected fluorescence. The fluorescence detection system further comprises a first detector configured to detect the fluorescence delivered by the receiving fiber to generate a response signal, and a processing unit configured to determine information about the samples by analyzing the response signal. Additionally, a fluorescence detection method is also presented.

Abstract translation: 荧光检测系统包括被配置为产生激发光，光学透镜和光纤束的光源。 光学透镜被配置为将激发光聚焦到样品以发射荧光并收集荧光。 纤维束探针包括被配置为将激发光透射到光学透镜的发射光纤和被配置为传送所收集的荧光的第一接收光纤。 荧光检测系统还包括第一检测器，其被配置为检测由接收光纤传送的荧光以产生响应信号;以及处理单元，被配置为通过分析响应信号来确定关于样本的信息。 另外，还提出了荧光检测方法。