公开(公告)号：US08911989B2

公开(公告)日：2014-12-16

申请号：US12795445

申请日：2010-06-07

Applicant: Gwo-Bin Lee ,  Sung-Yi Yang ,  Song-Bin Huang ,  Kang-Yi Lien ,  Chen-Hsun Weng

Inventor： Gwo-Bin Lee ,  Sung-Yi Yang ,  Song-Bin Huang ,  Kang-Yi Lien ,  Chen-Hsun Weng

IPC: C12M1/00 ,  C12P19/34 ,  B01L3/00 ,  B01F11/00 ,  B01F13/00 ,  F16K99/00 ,  B01L7/00

CPC classification number: F16K99/0001 ,  B01F11/0071 ,  B01F13/0059 ,  B01L3/50273 ,  B01L3/502738 ,  B01L7/52 ,  B01L2300/0819 ,  B01L2300/0867 ,  B01L2400/043 ,  B01L2400/049 ,  F16K99/0015 ,  F16K99/0059 ,  F16K2099/008 ,  F16K2099/0084 ,  Y10T137/0396

Abstract: The present invention provides a microfluidic biochip, which comprises: a fluid transportation unit having a fluid transportation reservoir and a fluid transportation air chamber; a first fluid storage reservoir; a second fluid storage reservoir; a first valve unit having a first valve and a first valve control air chamber; and a second valve unit having a second valve and a second valve control air chamber; wherein the first valve unit is located between the first fluid storage reservoir and the fluid transportation unit, the second valve unit is located between the second fluid storage reservoir and the fluid transportation unit, and the top portion of the fluid transportation reservoir and the valves are made of a flexible material. The structure of the present microfluidic biochip allows fluids to be transported and/or mixed therein.

Abstract translation: 本发明提供了一种微流体生物芯片，其包括：流体输送单元，其具有流体输送储存器和流体输送空气室; 第一流体储存容器; 第二流体储存容器; 第一阀单元，具有第一阀和第一阀控制空气室; 以及具有第二阀和第二阀控制空气室的第二阀单元; 其中所述第一阀单元位于所述第一流体储存容器和所述流体输送单元之间，所述第二阀单元位于所述第二流体储存容器和所述流体输送单元之间，并且所述流体输送储存器的顶部和所述阀为 由柔性材料制成。 本发明微流体生物芯片的结构允许流体在其中运输和/或混合。

公开(公告)号：US08624008B2

公开(公告)日：2014-01-07

申请号：US12924225

申请日：2010-09-22

Applicant: Gwo-Bin Lee ,  Shu-Chu Shiesh ,  Chao-June Huang ,  Hsin-I Lin

Inventor： Gwo-Bin Lee ,  Shu-Chu Shiesh ,  Chao-June Huang ,  Hsin-I Lin

IPC: C07H21/02

CPC classification number: G01N33/68 ,  C07K14/4737 ,  G01N2333/4737

Abstract: An aptamer specifically binding to C-reactive protein (CRP) is provided. The aptamer includes a following nucleotide sequence: 5′-angngggngnntgnnt-3′, wherein n is a nucleotide selected from a, t, c and g.

Abstract translation: 提供了特异性结合C反应蛋白（CRP）的适体。 适体包括以下核苷酸序列：5'-angngggngnntntntnt-3'，其中n是选自a，t，c和g的核苷酸。

公开(公告)号：US20100249770A1

公开(公告)日：2010-09-30

申请号：US12573665

申请日：2009-10-05

Applicant: Gwo-Bin Lee ,  Xi-Zhang Lin ,  Sheng-Chieh Huang

Inventor： Gwo-Bin Lee ,  Xi-Zhang Lin ,  Sheng-Chieh Huang

IPC: A61B18/18

CPC classification number: A61N1/403

Abstract: An electromagnetic thermotherapeutic system is adapted to treat a biological tissue and includes an electromagnetic thermotherapeutic apparatus and a high frequency induction heating circuit unit. The apparatus includes a needle assembly and a temperature monitor.The needle assembly is adapted to be inserted in the biological tissue, and includes a flexible and bendable needle seat, and a plurality of ferrous needles that are attached to the needle seat and that have needle tips extending in a direction away from the needle seat. The temperature monitor is connected to the needle assembly.The high frequency induction heating circuit unit has an induction coil capable of producing an electromagnetic field to induce currents in the ferrous needles and to thereby heat the ferrous needles.

Abstract translation: 电磁热治疗系统适于治疗生物组织，并且包括电磁热治疗装置和高频感应加热电路单元。 该装置包括针组件和温度监视器。 针组件适于插入生物组织中，并且包括柔性且可弯曲的针座，以及附接到针座并且具有沿远离针座的方向延伸的针尖的多个铁针。 温度监控器连接到针组件。 高频感应加热电路单元具有能够产生电磁场的感应线圈，以在铁针中引起电流，从而加热铁针。

公开(公告)号：US20080044894A1

公开(公告)日：2008-02-21

申请号：US11797052

申请日：2007-04-30

Applicant: Gwo-Bin Lee ,  Song-Bin Huang

Inventor： Gwo-Bin Lee ,  Song-Bin Huang

IPC: C12M1/00

CPC classification number: C12M23/16 ,  C12M29/10

Abstract: A cellular culture chip device includes a chip body. The chip body includes: a first flow channel having a first inlet end and a first outlet end and adapted to direct a liquid nutrient; a second flow channel adapted to direct a gellable culture medium; a medium retaining hole fluidly connected to the second flow channel and adapted to retain the gellable culture medium, the medium retaining hole having an opening connected fluidly to the first flow channel and adapted to expose the gellable culture medium to the liquid nutrient flowing through the first flow channel; a pump membrane adapted to control the flow of the liquid nutrient within the first flow channel; and a pressure channel unit to operate the pump membrane.

Abstract translation: 细胞培养芯片装置包括芯片体。 芯片主体包括：具有第一入口端和第一出口端并适于引导液体营养物的第一流动通道; 适于引导胶凝培养基的第二流动通道; 介质保持孔，其流体地连接到第二流动通道并且适于保持可凝胶化的培养基，所述介质保持孔具有与第一流动通道流体连接的开口，并适于将可凝胶培养基暴露于流过第一流动通道的液体营养物 流通道 泵膜，其适于控制所述第一流动通道内的液体营养物的流动; 以及用于操作泵膜的压力通道单元。

公开(公告)号：US20070004032A1

公开(公告)日：2007-01-04

申请号：US11316576

申请日：2005-12-21

Applicant: Gwo-Bin Lee ,  Chih-Hao Wang ,  Chia-Sheng Liao

Inventor： Gwo-Bin Lee ,  Chih-Hao Wang ,  Chia-Sheng Liao

IPC: C12M1/34

CPC classification number: B01L7/525 ,  B01L3/5027 ,  B01L3/502738 ,  B01L2200/147 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/087 ,  B01L2300/0887 ,  B01L2300/1827 ,  B01L2400/0481 ,  B01L2400/0605 ,  B01L2400/0638

Abstract: A chip device for a thermally cycled reaction includes a looped pressure channel divided into a plurality of angularly oriented sub-channels, and a looped fluid channel substantially corresponding to and disposed below the looped pressure channel. The fluid channel has a plurality of temperature zones below the respective sub-channels. A looped membrane is disposed between the fluid channel and the pressure channel. The membrane is inflated or deflated by the pressure in the sub-channels so as to block or unblock the fluid channel partially or completely. A fluid sample is movable by the membrane to an unblocked part of the fluid channel from a blocked part of the fluid channel so that the fluid sample may be circulated repeatedly along the fluid channel.

Abstract translation: 用于热循环反应的芯片装置包括分成多个成角度定向的子通道的环形压力通道和基本对应于并设置在环形压力通道下方的环形流体通道。 流体通道在相应的子通道下方具有多个温度区域。 环状膜布置在流体通道和压力通道之间。 膜通过子通道中的压力而膨胀或放气，以便部分地或完全地阻塞或解除流体通道的阻塞。 流体样品可以通过膜移动到来自流体通道的阻塞部分的流体通道的未阻挡部分，使得流体样品可以沿着流体通道重复循环。

公开(公告)号：US07142738B2

公开(公告)日：2006-11-28

申请号：US10658254

申请日：2003-09-10

Applicant: Gwo-Bin Lee

Inventor： Gwo-Bin Lee

IPC: G02B6/12 ,  C12P19/34

CPC classification number: B01L3/502715 ,  B01L3/502707 ,  B01L7/52 ,  B01L2200/147 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/1827 ,  B01L2400/0421 ,  G01N21/0303 ,  G01N2021/0325 ,  G01N2021/0346 ,  G02B6/262 ,  G02B6/3636 ,  G02B6/3644 ,  G02B6/3692 ,  G02B6/43

Abstract: The present invention provides an integrated analytical biochip and manufacturing method thereof, mainly by using a speedy and low-cost manufacturing process to integrate the Polymerase Chain Reaction (“PCR”) reaction tank, Capillary Electrophoresis (“CE”) channel and the fiber structure onto a single biochip, thus a DNA sample may be amplified via PCR on the biochip, and immediately driven into the CE channel for analysis in accordance with real-time online detection conducted through the fiber structure integrated on the biochip, such that a speedy and accurate process of biological sample detection and analysis can be achieved.

Abstract translation: 本发明提供了一种综合分析生物芯片及其制造方法，主要通过使用快速且低成本的制造方法将聚合酶链式反应（“PCR”）反应槽，毛细管电泳（“CE”）通道和纤维结构 在单个生物芯片上，因此可以通过PCR在生物芯片上扩增DNA样品，并且立即根据通过整合在生物芯片上的纤维结构进行的实时在线检测而立即进入CE通道进行分析，使得快速和 可以实现生物样品检测和分析的准确过程。

公开(公告)号：US20050274618A1

公开(公告)日：2005-12-15

申请号：US11133711

申请日：2005-05-21

Applicant: Gwo-Bin Lee ,  Che-Hsin Lin ,  Suz-Kai Hsiung

Inventor： Gwo-Bin Lee ,  Che-Hsin Lin ,  Suz-Kai Hsiung

IPC: G01N27/26 ,  G01N27/447

CPC classification number: G01N27/44721

Abstract: An assay chip includes a microchannel unit formed in a substrate. The microchannel unit includes a sample channel having inlet and outlet ends, a detection channel which intersects the sample channel and has an injection end and a recycle end on two opposite sides of the sample channel, respectively, and at least one light-exciting channel and at least one light-sensing channel disposed respectively adjacent to two opposite sides of the detection channel between the recycle end and the sample channel. A sensor unit includes first and second optical fibers inserted respectively into the light-exciting and light-sensing channels.

Abstract translation: 测定芯片包括形成在基板中的微通道单元。 微通道单元包括具有入口端和出口端的样品通道，与样品通道相交并具有分别在样品通道的两个相对侧上的注入端和再循环端的检测通道，以及至少一个光激发通道和 至少一个感光通道分别设置在再循环端和样品通道之间的邻近检测通道的两个相对侧。 传感器单元包括分别插入到光激励和光感测通道中的第一和第二光纤。

公开(公告)号：US08361060B2

公开(公告)日：2013-01-29

申请号：US12573665

申请日：2009-10-05

Applicant: Gwo-Bin Lee ,  Xi-Zhang Lin ,  Sheng-Chieh Huang

Inventor： Gwo-Bin Lee ,  Xi-Zhang Lin ,  Sheng-Chieh Huang

IPC: A61B18/04

CPC classification number: A61N1/403

Abstract: An electromagnetic thermotherapeutic system is adapted to treat a biological tissue and includes an electromagnetic thermotherapeutic apparatus and a high frequency induction heating circuit unit. The apparatus includes a needle assembly and a temperature monitor. The needle assembly is adapted to be inserted in the biological tissue and includes a flexible and bendable needle seat and a plurality of ferrous needles attached to the needle seat and having needle tips extending in a direction away from the needle seat. The temperature monitor is connected to the needle assembly. The high frequency induction heating circuit unit has an induction coil capable of producing an electromagnetic field to induce currents in the ferrous needles and to thereby heat the ferrous needles.

Abstract translation: 电磁热治疗系统适于治疗生物组织，并且包括电磁热治疗装置和高频感应加热电路单元。 该装置包括针组件和温度监视器。 针组件适于插入生物组织中，并且包括柔性且可弯曲的针座和附接到针座的多个铁针，并且具有沿远离针座的方向延伸的针尖。 温度监控器连接到针组件。 高频感应加热电路单元具有能够产生电磁场的感应线圈，以在铁针中引起电流，从而加热铁针。

公开(公告)号：US20120157749A1

公开(公告)日：2012-06-21

申请号：US12968293

申请日：2010-12-15

Applicant: Gwo-Bin Lee ,  Xi-Zhang Lin ,  Sheng-Chieh Huang ,  Yi-Yuan Chang ,  Chiung-Yu Chen ,  Yan-Shen Shan

Inventor： Gwo-Bin Lee ,  Xi-Zhang Lin ,  Sheng-Chieh Huang ,  Yi-Yuan Chang ,  Chiung-Yu Chen ,  Yan-Shen Shan

IPC: A61N2/00

CPC classification number: A61N1/403

Abstract: A heat therapy for thermally treating a target tissue within a living body by using a treating apparatus including a treating device with a magnetic part is provided. The magnetic part is heated to a first temperature by a high frequency electromagnetic field with a heating rate ranging from 1 to 5° C./sec, wherein the magnetic part is contacted the target tissue. Temperature controlling steps are performed to the magnetic part having the first temperature, and therefore the magnetic part has a final temperature higher than the first temperature, and a treatment is performed to the target tissue under the final temperature. Each of the temperature controlling steps is a heating step, a cooling step or a temperature conservation step, and the magnetic part is heated or cooled by the high frequency electromagnetic field with a heating rate or a cooling rate ranging from 1 to 5° C./sec.

Abstract translation: 提供了一种通过使用包括具有磁性部件的处置装置的处理装置对生物体内的靶组织进行热处理的热疗法。 通过加热速率为1〜5℃/秒的高频电磁场将磁性部件加热至第一温度，其中磁性部件与目标组织接触。 对具有第一温度的磁性部件进行温度控制步骤，因此磁性部件的最终温度高于第一温度，并且在最终温度下对目标组织进行处理。 每个温度控制步骤是加热步骤，冷却步骤或温度保持步骤，并且通过高频电磁场以1至5℃的加热速率或冷却速率将磁性部件加热或冷却。 /秒。

公开(公告)号：US20110318846A1

公开(公告)日：2011-12-29

申请号：US12924225

申请日：2010-09-22

Applicant: Gwo-Bin Lee ,  Shu-Chu Shiesh ,  Chao-June Huang ,  Hsin-I Lin

Inventor： Gwo-Bin Lee ,  Shu-Chu Shiesh ,  Chao-June Huang ,  Hsin-I Lin

IPC: G01N33/566 ,  C12N9/00 ,  C07H21/00

CPC classification number: G01N33/68 ,  C07K14/4737 ,  G01N2333/4737

Abstract: An aptamer specifically binding to C-reactive protein (CRP) is provided. The aptamer includes a following nucleotide sequence: 5′-angngggngnntgnnt-3′, wherein n is a nucleotide selected from a, t, c and g.

Abstract translation: 提供了特异性结合C反应蛋白（CRP）的适体。 适体包括以下核苷酸序列：5'-angngggngnntntntnt-3'，其中n是选自a，t，c和g的核苷酸。