公开(公告)号：US20060228256A1

公开(公告)日：2006-10-12

申请号：US10544954

申请日：2004-02-09

Applicant: John McDevitt ,  Adrain Goodey ,  Jason Shear ,  Eric Anslyn ,  Dean Neikirk

Inventor： John McDevitt ,  Adrain Goodey ,  Jason Shear ,  Eric Anslyn ,  Dean Neikirk

IPC: G01N21/00

CPC classification number: G01N21/6458 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00423 ,  B01J2219/00468 ,  B01J2219/005 ,  B01J2219/00545 ,  B01J2219/00576 ,  B01J2219/00648 ,  B01J2219/00702 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01L3/5025 ,  B01L3/502715 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2300/021 ,  B01L2300/0816 ,  B01L2400/0638 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  G01N15/1463 ,  G01N21/05 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/6454 ,  G01N33/5432 ,  G01N33/545 ,  G01N2021/0346

Abstract: The development of miniaturized chromatographic systems localized within individual polymer microspheres and their incorporation into a bead-based cross-reactive sensor array platform is described herein. The integrated chromatographic and detection concept is based on the creation of distinct functional layers within the microspheres. In this first example of the new methodology, complexing ligands have been selectively immobilized to create “separation” layers harboring an affinity for various analytes. Information concerning the identities and concentrations of analytes may be drawn from the temporal properties of the beads' optical responses. Varying the nature of the ligand in the separation shell yields a collection of cross-reactive sensing elements well suited for use in array-based micro-total-analysis systems.

Abstract translation: 本文描述了位于单个聚合物微球体内的小型化色谱系统的发展及其掺入到基于珠的交叉反应性传感器阵列平台中。 集成的色谱和检测概念是基于在微球内产生不同的功能层。 在新方法的第一个例子中，已经选择性地固定络合配体以产生对各种分析物具有亲和性的“分离”层。 关于分析物的身份和浓度的信息可以从珠的光学响应的​​时间特性中得出。 改变分离壳中配体的性质产生了非常适合用于基于阵列的微全分析系统的交叉反应感测元件的集合。

公开(公告)号：US08101431B2

公开(公告)日：2012-01-24

申请号：US11020443

申请日：2004-12-22

Applicant: John T. McDevitt ,  Karri L. Ballard ,  Pierre N. Floriano ,  Nick J. Christodoulides ,  Dean Neikirk ,  Eric Anslyn ,  Jason Shear

Inventor： John T. McDevitt ,  Karri L. Ballard ,  Pierre N. Floriano ,  Nick J. Christodoulides ,  Dean Neikirk ,  Eric Anslyn ,  Jason Shear

IPC: G01N33/53

CPC classification number: G01N21/251 ,  B01L3/502715 ,  B01L3/502738 ,  B01L9/527 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2200/0684 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/0887 ,  B01L2400/0406 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0688 ,  G01N21/6428 ,  G01N21/6452 ,  G01N2021/6419 ,  G01N2021/6441 ,  Y10S436/809

Abstract: Described herein is an analyte detection device and method related to a portable instrument suitable for point-of-care analyses. In some embodiments, a portable instrument may include a disposable cartridge, an optical detector, a sample collection device and/or sample reservoir, reagent delivery systems, fluid delivery systems, one or more channels, and/or waste reservoirs. Use of a portable instrument may reduce the hazard to an operator by reducing an operator's contact with a sample for analysis. The device is capable of obtaining diagnostic information using cellular- and/or particle-based analyses and may be used in conjunction with membrane- and/or particle-based analysis cartridges. Analytes, including proteins and cells and/or microbes may be detected using the membrane and/or particle based analysis system.

Abstract translation: 本文描述了与适合于点护理分析的便携式仪器相关的分析物检测装置和方法。 在一些实施例中，便携式仪器可以包括一次性盒，光学检测器，样品收集装置和/或样品储存器，试剂递送系统，流体输送系统，一个或多个通道和/或废物储存器。 使用便携式仪器可能会降低操作员与样品的接触以分析危害操作员的危险。 该设备能够使用基于细胞和/或基于粒子的分析获得诊断信息，并且可以与基于膜和/或基于粒子的分析盒结合使用。 可以使用基于膜和/或基于粒子的分析系统检测分析物，包括蛋白质和细胞和/或微生物。

公开(公告)号：US20050214863A1

公开(公告)日：2005-09-29

申请号：US11010816

申请日：2004-12-13

Applicant: John McDevitt ,  Eric Anslyn ,  Jason Shear ,  Dean Neikirk ,  Nick Christodoulides

Inventor： John McDevitt ,  Eric Anslyn ,  Jason Shear ,  Dean Neikirk ,  Nick Christodoulides

IPC: B01J19/00 ,  B01L3/00 ,  C40B40/10 ,  C40B60/14 ,  G01N21/64 ,  G01N21/77 ,  G01N33/53 ,  G01N33/543 ,  G01N33/544 ,  G01N33/552 ,  G01N33/68 ,  G01N33/92

CPC classification number: G01N33/5304 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/005 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00702 ,  B01J2219/00725 ,  B01L3/5025 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/502738 ,  B01L3/502761 ,  B01L2300/0819 ,  B01L2300/0877 ,  B01L2400/0487 ,  C40B40/10 ,  C40B60/14 ,  G01N21/6428 ,  G01N21/6454 ,  G01N21/77 ,  G01N33/54313 ,  G01N33/544 ,  G01N33/552 ,  G01N33/6887 ,  G01N33/92 ,  G01N2021/7783 ,  G01N2021/7786 ,  G01N2201/024 ,  G01N2800/32 ,  Y10S436/807 ,  Y10S436/809

Abstract: A system for the rapid characterization of analytes in saliva. In one embodiment, a system for detecting analytes includes a light source, a sensor array, and a detector. The sensor array is formed from a supporting member, in which a plurality of cavities may be formed. A series of chemically sensitive particles, in one embodiment, are positioned within the cavities. The particles may produce a signal when a receptor, coupled to the particle, interacts with the cardiovascular risk factor analyte and the particle-analyte complex is visualized using a visualization reagent. Using pattern recognition techniques, the analytes within a multi-analyte fluid may be characterized. In an embodiment, each cavity of the plurality of cavities is designed to capture and contain a specific size particle. Flexible projections may be positioned over each of the cavities to provide retention of the particles in the cavities.

Abstract translation: 用于快速表征唾液中分析物的系统。 在一个实施例中，用于检测分析物的系统包括光源，传感器阵列和检测器。 传感器阵列由可以形成多个空腔的支撑构件形成。 在一个实施方案中，一系列化学敏感性颗粒位于空腔内。 当与颗粒偶联的受体与心血管危险因子分析物相互作用并且使用可视化试剂显现颗粒 - 分析物复合物时，颗粒可产生信号。 使用模式识别技术，可以对多分析物流体中的分析物进行表征。 在一个实施例中，多个空腔的每个空腔被设计成捕获并容纳特定尺寸的颗粒。 可以将柔性突出部定位在每个空腔上，以提供颗粒在空腔中的保持。

公开(公告)号：US20050164320A1

公开(公告)日：2005-07-28

申请号：US11039054

申请日：2005-01-20

Applicant: John McDevitt ,  Eric Anslyn ,  Jason Shear ,  Dean Neikirk

Inventor： John McDevitt ,  Eric Anslyn ,  Jason Shear ,  Dean Neikirk

IPC: C40B60/14 ,  G01N21/77 ,  G01N33/53 ,  C12M1/34 ,  G01N33/537 ,  G01N33/543

CPC classification number: G01N21/77 ,  B01J2219/00317 ,  B01J2219/00648 ,  B01J2219/00659 ,  C40B60/14 ,  Y10S435/808 ,  Y10S435/973 ,  Y10S436/80 ,  Y10S436/805 ,  Y10S436/807 ,  Y10S436/809 ,  Y10T436/25

Abstract: A system for the rapid characterization of multi-analyte fluids, in one embodiment, includes a light source, a sensor array, and a detector. The sensor array is formed from a supporting member into which a plurality of cavities may be formed. A series of chemically sensitive particles are, in one embodiment positioned within the cavities. The particles may be configured to produce a signal when a receptor coupled to the particle interacts with the analyte. Using pattern recognition techniques, the analytes within a multi-analyte fluid may be characterized.

公开(公告)号：US20050136548A1

公开(公告)日：2005-06-23

申请号：US10924285

申请日：2004-08-23

Applicant: John McDevitt ,  Eric Anslyn ,  Jason Shear ,  Dean Neikirk

Inventor： John McDevitt ,  Eric Anslyn ,  Jason Shear ,  Dean Neikirk

IPC: B01L3/00 ,  B81B1/00 ,  B81B3/00 ,  C07B61/00 ,  G01N1/02 ,  G01N21/25 ,  G01N21/64 ,  G01N21/77 ,  G01N33/48 ,  G01N33/53 ,  G01N33/537 ,  G01N33/543 ,  G01N33/554 ,  G01N33/569 ,  G01N35/00 ,  G06F19/00 ,  H01L21/00 ,  G01N1/10

CPC classification number: G01N21/6452 ,  B01J2219/005 ,  B01J2219/00576 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00702 ,  B01J2219/00722 ,  B01L3/0289 ,  B01L3/5025 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502761 ,  B01L2200/027 ,  B01L2200/0657 ,  B01L2200/0668 ,  B01L2200/12 ,  B01L2300/0636 ,  B01L2400/0415 ,  B01L2400/049 ,  B01L2400/0633 ,  B01L2400/0638 ,  C07B2200/11 ,  C12Q1/34 ,  C12Q1/37 ,  C40B40/00 ,  G01N21/253 ,  G01N21/6428 ,  G01N21/6454 ,  G01N21/6458 ,  G01N21/6486 ,  G01N21/77 ,  G01N33/54313 ,  G01N33/54366 ,  G01N33/54373 ,  G01N2001/021 ,  G01N2021/6417 ,  G01N2021/6421 ,  G01N2021/6432 ,  G01N2021/6439 ,  G01N2021/6441 ,  G01N2021/6482 ,  G01N2035/00158 ,  G01N2035/00564 ,  G01N2201/062 ,  G01N2201/0627 ,  Y02A90/26 ,  Y10S977/924 ,  Y10T436/2575

Abstract: A system for the rapid characterization of multi-analyte fluids, in one embodiment, includes a light source, a sensor array, and a detector. The sensor array is formed from a supporting member into which a plurality of cavities may be formed. A series of chemically sensitive particles are, in one embodiment positioned within the cavities. The particles may be configured to produce a signal when a receptor coupled to the particle interacts with the analyte. Using pattern recognition techniques, the analytes within a multi-analyte fluid may be characterized.

Abstract translation: 在一个实施例中，用于快速表征多分析物流体的系统包括光源，传感器阵列和检测器。 传感器阵列由可以形成多个空腔的支撑构件形成。 在一个实施例中，一系列化学敏感的颗粒位于空腔内。 当与颗粒偶联的受体与分析物相互作用时，颗粒可被配置成产生信号。 使用模式识别技术，可以对多分析物流体中的分析物进行表征。

公开(公告)号：US20060257992A1

公开(公告)日：2006-11-16

申请号：US11020443

申请日：2004-12-22

Applicant: John McDevitt ,  Karri Ballard ,  Pierre Floriano ,  Nick Christodoulides ,  Dean Neikirk ,  Eric Anslyn ,  Jason Shear

Inventor： John McDevitt ,  Karri Ballard ,  Pierre Floriano ,  Nick Christodoulides ,  Dean Neikirk ,  Eric Anslyn ,  Jason Shear

IPC: C12M1/34

CPC classification number: G01N21/251 ,  B01L3/502715 ,  B01L3/502738 ,  B01L9/527 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2200/0684 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/0887 ,  B01L2400/0406 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0688 ,  G01N21/6428 ,  G01N21/6452 ,  G01N2021/6419 ,  G01N2021/6441 ,  Y10S436/809

Abstract: Described herein is an analyte detection device and method related to a portable instrument suitable for point-of-care analyses. In some embodiments, a portable instrument may include a disposable cartridge, an optical detector, a sample collection device and/or sample reservoir, reagent delivery systems, fluid delivery systems, one or more channels, and/or waste reservoirs. Use of a portable instrument may reduce the hazard to an operator by reducing an operator's contact with a sample for analysis. The device is capable of obtaining diagnostic information using cellular- and/or particle-based analyses and may be used in conjunction with membrane- and/or particle-based analysis cartridges. Analytes, including proteins and cells and/or microbes may be detected using the membrane and/or particle based analysis system.

Abstract translation: 本文描述了与适合于点护理分析的便携式仪器相关的分析物检测装置和方法。 在一些实施例中，便携式仪器可以包括一次性盒，光学检测器，样品收集装置和/或样品储存器，试剂递送系统，流体输送系统，一个或多个通道和/或废物储存器。 使用便携式仪器可能会降低操作员与样品的接触以分析危害操作员的危险。 该设备能够使用基于细胞和/或基于粒子的分析获得诊断信息，并且可以与基于膜和/或基于粒子的分析盒结合使用。 可以使用基于膜和/或基于粒子的分析系统检测分析物，包括蛋白质和细胞和/或微生物。

公开(公告)号：US20120135396A1

公开(公告)日：2012-05-31

申请号：US13303525

申请日：2011-11-23

Applicant: John T. McDevitt ,  Adrian Goodey ,  Jason Shear ,  Eric Anslyn ,  Dean P. Neikirk

Inventor： John T. McDevitt ,  Adrian Goodey ,  Jason Shear ,  Eric Anslyn ,  Dean P. Neikirk

IPC: G01N33/53 ,  G01N21/78 ,  G01N33/569

CPC classification number: G01N21/6458 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00423 ,  B01J2219/00468 ,  B01J2219/005 ,  B01J2219/00545 ,  B01J2219/00576 ,  B01J2219/00648 ,  B01J2219/00702 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01L3/5025 ,  B01L3/502715 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2300/021 ,  B01L2300/0816 ,  B01L2400/0638 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  G01N15/1463 ,  G01N21/05 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/6454 ,  G01N33/5432 ,  G01N33/545 ,  G01N2021/0346

Abstract: The development of miniaturized chromatographic systems localized within individual polymer microspheres and their incorporation into a bead-based cross-reactive sensor array platform is described herein. The integrated chromatographic and detection concept is based on the creation of distinct functional layers within the microspheres. In this first example of the new methodology, complexing ligands have been selectively immobilized to create “separation” layers harboring an affinity for various analytes. Information concerning the identities and concentrations of analytes may be drawn from the temporal properties of the beads' optical responses, Varying the nature of the ligand in the separation shell yields a collection of cross-reactive sensing elements well suited for use in array-based micro-total-analysis systems.

Abstract translation: 本文描述了位于单个聚合物微球体内的小型化色谱系统的发展及其掺入到基于珠的交叉反应性传感器阵列平台中。 集成的色谱和检测概念是基于在微球内产生不同的功能层。 在新方法的第一个例子中，已经选择性地固定络合配体以产生对各种分析物具有亲和性的“分离”层。 关于分析物的身份和浓度的信息可以从珠的光学响应的​​时间特性中得出，改变分离壳中配体的性质产生了非常适合用于基于阵列的微观的交叉反应性感测元件的集合 总分析系统。

公开(公告)号：US08105849B2

公开(公告)日：2012-01-31

申请号：US11022176

申请日：2004-12-22

Applicant: John T. McDevitt ,  Karri L. Ballard ,  Pierre N. Floriano ,  Nick J. Christodoulides ,  Dean Neikirk ,  Eric Anslyn ,  Jason Shear

Inventor： John T. McDevitt ,  Karri L. Ballard ,  Pierre N. Floriano ,  Nick J. Christodoulides ,  Dean Neikirk ,  Eric Anslyn ,  Jason Shear

IPC: G01N33/53

CPC classification number: B01L3/502738 ,  B01L3/502715 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2200/16 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/0887 ,  B01L2400/0406 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0655 ,  G01N21/6428 ,  Y10S436/809

Abstract: Described herein is an analyte detection device and method related to a portable instrument suitable for point-of-care analyses. In some embodiments, a portable instrument may include a disposable cartridge, an optical detector, a sample collection device and/or sample reservoir, reagent delivery systems, fluid delivery systems, one or more channels, and/or waste reservoirs. Use of a portable instrument may reduce the hazard to an operator by reducing an operator's contact with a sample for analysis. The device is capable of obtaining diagnostic information using cellular- and/or particle-based analyses and may be used in conjunction with membrane- and/or particle-based analysis cartridges. Analytes, including proteins and cells and/or microbes may be detected using the membrane and/or particle based analysis system.

Abstract translation: 本文描述了与适合于点护理分析的便携式仪器相关的分析物检测装置和方法。 在一些实施例中，便携式仪器可以包括一次性盒，光学检测器，样品收集装置和/或样品储存器，试剂递送系统，流体输送系统，一个或多个通道和/或废物储存器。 使用便携式仪器可能会降低操作员与样品的接触以分析危害操作员的危险。 该设备能够使用基于细胞和/或基于粒子的分析获得诊断信息，并且可以与基于膜和/或基于粒子的分析盒结合使用。 可以使用基于膜和/或基于粒子的分析系统检测分析物，包括蛋白质和细胞和/或微生物。

公开(公告)号：US20060257993A1

公开(公告)日：2006-11-16

申请号：US11022176

申请日：2004-12-22

Applicant: John McDevitt ,  Karri Ballard ,  Pierre Floriano ,  Nick Christodoulides ,  Dean Neikirk ,  Eric Anslyn ,  Jason Shear

Inventor： John McDevitt ,  Karri Ballard ,  Pierre Floriano ,  Nick Christodoulides ,  Dean Neikirk ,  Eric Anslyn ,  Jason Shear

IPC: C12M1/34

CPC classification number: B01L3/502738 ,  B01L3/502715 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2200/16 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/0887 ,  B01L2400/0406 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0655 ,  G01N21/6428 ,  Y10S436/809

Abstract: Described herein is an analyte detection device and method related to a portable instrument suitable for point-of-care analyses. In some embodiments, a portable instrument may include a disposable cartridge, an optical detector, a sample collection device and/or sample reservoir, reagent delivery systems, fluid delivery systems, one or more channels, and/or waste reservoirs. Use of a portable instrument may reduce the hazard to an operator by reducing an operator's contact with a sample for analysis. The device is capable of obtaining diagnostic information using cellular- and/or particle-based analyses and may be used in conjunction with membrane- and/or particle-based analysis cartridges. Analytes, including proteins and cells and/or microbes may be detected using the membrane and/or particle based analysis system.

Abstract translation: 本文描述了与适合于点护理分析的便携式仪器相关的分析物检测装置和方法。 在一些实施例中，便携式仪器可以包括一次性盒，光学检测器，样品收集装置和/或样品储存器，试剂递送系统，流体输送系统，一个或多个通道和/或废物储存器。 使用便携式仪器可能会降低操作员与样品的接触以分析危害操作员的危险。 该设备能够使用基于细胞和/或基于粒子的分析获得诊断信息，并且可以与基于膜和/或基于粒子的分析盒结合使用。 可以使用基于膜和/或基于粒子的分析系统检测分析物，包括蛋白质和细胞和/或微生物。

公开(公告)号：US20060257991A1

公开(公告)日：2006-11-16

申请号：US11020442

申请日：2004-12-22

Applicant: John McDevitt ,  Karri Ballard ,  Pierre Floriano ,  Nick Christodoulides ,  Dean Neikirk ,  Eric Anslyn ,  Jason Shear

Inventor： John McDevitt ,  Karri Ballard ,  Pierre Floriano ,  Nick Christodoulides ,  Dean Neikirk ,  Eric Anslyn ,  Jason Shear

IPC: C12M1/34

CPC classification number: C12M41/36

Abstract: Described herein is an analyte detection device and method related to a portable instrument suitable for point-of-care analyses. In some embodiments, a portable instrument may include a disposable cartridge, an optical detector, a sample collection device and/or sample reservoir, reagent delivery systems, fluid delivery systems, one or more channels, and/or waste reservoirs. Use of a portable instrument may reduce the hazard to an operator by reducing an operator's contact with a sample for analysis. The device is capable of obtaining diagnostic information using cellular- and/or particle-based analyses and may be used in conjunction with membrane- and/or particle-based analysis cartridges. Analytes, including proteins and cells and/or microbes may be detected using the membrane and/or particle based analysis system.

Abstract translation: 本文描述了与适合于点护理分析的便携式仪器相关的分析物检测装置和方法。 在一些实施例中，便携式仪器可以包括一次性盒，光学检测器，样品收集装置和/或样品储存器，试剂递送系统，流体输送系统，一个或多个通道和/或废物储存器。 使用便携式仪器可能会降低操作员与样品的接触以分析危害操作员的危险。 该设备能够使用基于细胞和/或基于粒子的分析获得诊断信息，并且可以与基于膜和/或基于粒子的分析盒结合使用。 可以使用基于膜和/或基于粒子的分析系统检测分析物，包括蛋白质和细胞和/或微生物。