公开(公告)号：US20150316533A1

公开(公告)日：2015-11-05

申请号：US14696756

申请日：2015-04-27

Applicant: Josef Kerimo ,  Hansong Zeng ,  Ron Scharlack ,  Gert Blankenstein

Inventor： Josef Kerimo ,  Hansong Zeng ,  Ron Scharlack ,  Gert Blankenstein

IPC: G01N33/49 ,  G01N21/65 ,  G01N21/64

CPC classification number: G01N21/534 ,  G01N21/59 ,  G01N21/6408 ,  G01N21/6486 ,  G01N21/65 ,  G01N33/4905 ,  G01N2201/062 ,  G01N2201/0636

Abstract: This invention relates to an optical system and method for performing turbidity assay, e.g. coagulation of blood or plasma, comprising a standard optical reference, a sample handling structure, a light source and an optical detection unit. The standard optical reference, such as a fluorophore-doped glass, provides constant optical signal under controlled optical conditions. The sample handling structure, such as a microfluidic system with reaction chamber, can be placed beneath or above the standard optical reference. During operation, the coagulating plasma/blood changes its optical absorbance and reflection properties, which results in changes in optical signal that reaches the optical reading unit. The variation of the optical signal, such as fluorescence signal indicates the kinetics of the turbidity varying process, such as plasma/blood coagulation process. This invention is used for performing turbidity assay with optical system, including photometry system, fluorescence system, Raman Spectroscopy system and so on.

Abstract translation: 本发明涉及一种用于进行浊度测定的光学系统和方法，例如， 血液或血浆的凝结，包括标准光学参考，样品处理结构，光源和光学检测单元。 诸如荧光团掺杂玻璃的标准光学参考在受控的光学条件下提供恒定的光学信号。 样品处理结构，如具有反应室的微流体系统，可放置在标准光学参考下方或上方。 在操作过程中，凝固血浆/血液会改变其光吸收和反射特性，导致到达光学读取单元的光信号的变化。 光信号的变化，例如荧光信号表示浊度变化过程的动力学，例如血浆/血液凝固过程。 本发明用于光学系统进行浊度测定，包括光度测定系统，荧光系统，拉曼光谱系统等。

公开(公告)号：US20150268237A1

公开(公告)日：2015-09-24

申请号：US14663560

申请日：2015-03-20

Applicant: Josef Kerimo ,  Hansong Zeng ,  Ron Scharlack ,  Gert Blankenstein

Inventor： Josef Kerimo ,  Hansong Zeng ,  Ron Scharlack ,  Gert Blankenstein

IPC: G01N33/543 ,  G01N21/41

CPC classification number: G01N33/54373 ,  G01N21/658 ,  G01N21/7743 ,  G01N33/54346 ,  G01N33/588

Abstract: A bioserisor platform is configured with upconverting nanoparticles on the surface of a resonant grating structure with enhanced sensitivity. The grating structure is illuminated with a light beam at one of its resonance modes to form a strong evanescent field at the surface and used for high sensitivity assays. The strong evanescent field triggers the upconverting nanoparticles to generate enhanced and localized emission on the grating surface, with lower background and lower auto-fluorescence from the grating substrate. This leads to improved performance in detecting analytes in bioassays.

Abstract translation: 生物传感器平台配置为在谐振光栅结构的表面上上变换纳米颗粒，具有增强的灵敏度。 光栅结构以其共振模式之一的光束照射，以在表面形成强烈的消逝场，并用于高灵敏度测定。 强衰减场触发上变换纳米粒子，以在光栅表面产生增强和局部发射，具有较低的背景和较低的光栅衬底的自发荧光。 这导致在生物测定中检测分析物方面的改进性能。