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    Vessel, preferably spherical or oblate spherical for growing or culturing cells, cellular aggregates, tissues and organoids and methods for using same
    1.
    发明授权
    Vessel, preferably spherical or oblate spherical for growing or culturing cells, cellular aggregates, tissues and organoids and methods for using same 有权
    容器，优选球形或扁球形，用于生长或培养细胞，细胞聚集体，组织和组织，以及使用它们的方法

    公开(公告)号：US06642019B1

    公开(公告)日：2003-11-04

    申请号：US09718814

    申请日：2000-11-22

    Applicant: Charles Daniel Anderson ,  Charlie W. Dodd ,  Mark Stuart Anderson

    Inventor： Charles Daniel Anderson ,  Charlie W. Dodd ,  Mark Stuart Anderson

    IPC: C12P100

    CPC classification number: C12M23/02 ,  C12M27/10 ,  C12M29/04 ,  C12M29/10

    Abstract: An improved culture vessel or bioreactor for growing cells, cellular aggregates, tissue particles or tissue “organoids” and methods for using same which includes a housing having at least one chamber defined by a curved wall symmetrical about an axis. The perfused version of the vessel has at least one inlet and at least one outlet and at least one filter in fluid communication with at least one of the inlets or outlets structured to pass culture media but retain cells, cellular aggregates or tissues within the culture chamber. The batch culture version of the vessel has at least part of the vessel wall made of a gas permeable material. The vessel housing is rotated about the horizontal or proximate horizontal axis to suspend the cells, in an unobstructed, three-dimensional or toroidal flow. The chamber has a substantially ellipsoid, oblate ellipsoid, spherical or oblate spherical shape and an unobstructed volume for growth of cells, cellular aggregates or tissues. The perfused version of the invention has a pump in fluid communication with an inlet or outlet to maintain a flow of oxygen and nutrient rich culture media through the vessel to sustain respiration and metabolism. A gas exchange device is used to maintain desired concentration of gases. Operational modes include either continuous perfusion flow or a batch culture. Methods are disclosed to produce biological products, cells and tissues and to filter waste materials and toxins from fluids (such as a liver assist device).

    Abstract translation: 用于生长细胞，细胞聚集体，组织颗粒或组织“有机体”的改进的培养容器或生物反应器及其使用方法，其包括壳体，所述壳体具有由围绕轴对称的弯曲壁限定的至少一个室。 所述血管的灌注版本具有至少一个入口和至少一个出口以及与至少一个入口或出口流体连通的至少一个过滤器，所述至少一个入口或出口被构造成通过培养基，但保留在培养室内的细胞，细胞聚集体或组织 。 容器的批量培养版具有由透气材料制成的容器壁的至少一部分。 容器壳体围绕水平或近似的水平轴线旋转以将细胞悬挂在无障碍的三维或环形流中。 腔室具有基本上椭圆形的扁圆形椭圆形，球形或扁圆球形，以及用于细胞，细胞聚集体或组织生长的无障碍体积。 本发明的灌注版本具有与入口或出口流体连通的泵，以通过血管维持氧气和营养丰富的培养基流，以维持呼吸和新陈代谢。 气体交换装置用于保持所需的气体浓度。 操作模式包括连续灌注流或分批培养。 公开了生产生物制品，细胞和组织以及从流体（例如肝脏辅助装置）中过滤废物和毒素的方法。

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