DNase expression in recombinant host cells
    1.
    发明授权
    DNase expression in recombinant host cells 有权
    DNase在重组宿主细胞中的表达

    公开(公告)号:US08227227B2

    公开(公告)日:2012-07-24

    申请号:US12516720

    申请日:2007-11-30

    CPC classification number: C12N1/08 C12N9/22 C12N15/75

    Abstract: The present invention relates to cells producing at least one polypeptide of interest and expressing one or more recombinant nuclease encoding gene(s) thereby producing the nuclease(s), and methods for producing a polypeptide of interest essentially free from contaminating DNA, said method comprising the steps of: (a) cultivating a cell that produces at least one polypeptide of interest and expresses one or more recombinant nuclease encoding gene(s) thereby producing the nuclease(s); and (b) isolating the polypeptide of interest.

    Abstract translation: 本发明涉及产生感兴趣的至少一种目的多肽并表达一种或多种重组核酸酶编码基因从而产生核酸酶的细胞,以及用于生产基本上不含污染DNA的目标多肽的方法,所述方法包括 步骤:(a)培养产生至少一种目的多肽的细胞,并表达一种或多种重组核酸酶编码基因从而产生核酸酶; 和(b)分离感兴趣的多肽。

    DNase expression in recombinant host cells
    2.
    发明授权
    DNase expression in recombinant host cells 有权
    DNase在重组宿主细胞中的表达

    公开(公告)号:US08569029B2

    公开(公告)日:2013-10-29

    申请号:US13532268

    申请日:2012-06-25

    CPC classification number: C12N1/08 C12N9/22 C12N15/75

    Abstract: The present invention relates to cells producing at least one polypeptide of interest and expressing one or more recombinant nuclease encoding gene(s) thereby producing the nuclease(s), and methods for producing a polypeptide of interest essentially free from contaminating DNA, said method comprising the steps of: (a) cultivating a cell that produces at least one polypeptide of interest and expresses one or more recombinant nuclease encoding gene(s) thereby producing the nuclease(s); and (b) isolating the polypeptide of interest.

    Abstract translation: 本发明涉及产生感兴趣的至少一种目的多肽并表达一种或多种重组核酸酶编码基因从而产生核酸酶的细胞,以及用于生产基本上不含污染DNA的目标多肽的方法,所述方法包括 步骤:(a)培养产生至少一种目的多肽的细胞,并表达一种或多种重组核酸酶编码基因从而产生核酸酶; 和(b)分离感兴趣的多肽。

    Device for an extendable reception of a ramp
    3.
    发明申请
    Device for an extendable reception of a ramp 审中-公开
    可扩展接收斜坡的装置

    公开(公告)号:US20120030886A1

    公开(公告)日:2012-02-09

    申请号:US13118698

    申请日:2011-05-31

    CPC classification number: B60R3/02

    Abstract: Subject matter of the invention is a device for an extendable reception of a ramp, wherein the device can be mounted at a vehicle in the area of the vehicle door, wherein the device comprises a drive assembly for the ramp, wherein the drive assembly comprises a slide, wherein the slide is connected with the ramp, wherein the drive equipment can be engaged with at least one power transmission element mounted at the vehicle.

    Abstract translation: 本发明的主题是用于可延伸接收斜坡的装置,其中装置可以安装在车辆门的区域中的车辆处,其中该装置包括用于斜坡的驱动组件,其中驱动组件包括一个 滑动件,其中所述滑块与所述斜坡连接,其中所述驱动设备可与安装在所述车辆上的至少一个动力传动元件接合。

    DNASE EXPRESSION IN RECOMBINANT HOST CELLS
    4.
    发明申请
    DNASE EXPRESSION IN RECOMBINANT HOST CELLS 有权
    重组质粒细胞中的DNASE表达

    公开(公告)号:US20120264169A1

    公开(公告)日:2012-10-18

    申请号:US13532268

    申请日:2012-06-25

    CPC classification number: C12N1/08 C12N9/22 C12N15/75

    Abstract: The present invention relates to cells producing at least one polypeptide of interest and expressing one or more recombinant nuclease encoding gene(s) thereby producing the nuclease(s), and methods for producing a polypeptide of interest essentially free from contaminating DNA, said method comprising the steps of: (a) cultivating a cell that produces at least one polypeptide of interest and expresses one or more recombinant nuclease encoding gene(s) thereby producing the nuclease(s); and (b) isolating the polypeptide of interest.

    Abstract translation: 本发明涉及产生感兴趣的至少一种目的多肽并表达一种或多种重组核酸酶编码基因从而产生核酸酶的细胞,以及用于生产基本上不含污染DNA的目标多肽的方法,所述方法包括 步骤:(a)培养产生至少一种目的多肽的细胞,并表达一种或多种重组核酸酶编码基因从而产生核酸酶; 和(b)分离感兴趣的多肽。

    BALL JOINT DEVICE
    5.
    发明申请
    BALL JOINT DEVICE 有权
    球联合装置

    公开(公告)号:US20090136290A1

    公开(公告)日:2009-05-28

    申请号:US12276181

    申请日:2008-11-21

    Applicant: Martin PERSSON

    Inventor: Martin PERSSON

    Abstract: This invention relates to a double ball joint device comprising: a housing; a first joint element comprising a first ball, wherein a major portion of said first ball is mounted and retained within said housing and a minor portion of the first ball protrudes from said housing through a first opening thereof, and a first shaft, which is attached to the first ball at said minor portion thereof; a second joint element comprising a second ball, wherein a major portion of said second ball is mounted and retained within said housing and a minor portion of the second ball protrudes from said housing through a second opening thereof, and a second shaft, which is attached to the second ball at said minor portion thereof; a locking washer, which is arranged between said first and second balls and which comprises a first seat for receiving said first ball, and an opposite second seat for receiving said second ball, and an operation member, which is operable between a locked state where it urges said locking washer to clamp lock said first and second joint elements in a current position, and a released state where it releases said locking washer thereby releasing the joint elements, wherein the joint elements are pivotable, at said released state, into a desired position.

    Abstract translation: 双球接头装置技术领域本发明涉及一种双球接头装置,其包括:壳体; 第一接头元件,包括第一球,其中所述第一球的主要部分被安装并保持在所述壳体内,并且所述第一球的小部分通过其第一开口从所述壳体突出,并且第一轴被附接 到其所述小部分的第一个球; 第二接头元件,包括第二球,其中所述第二球的主要部分被安装并保持在所述壳体内,并且所述第二球的小部分通过其第二开口从所述壳体突出,并且第二轴被附接 在所述次要部分处的第二球; 锁定垫圈,其布置在所述第一和第二球之间,并且包括用于接收所述第一球的第一座和用于接收所述第二球的相对的第二座,以及操作构件,所述操作构件可在其锁定状态 推动所述锁紧垫圈将所述第一和第二接头元件夹紧在当前位置,以及释放状态,在该状态下释放所述锁定垫圈从而释放所述接合元件,其中所述接合元件在所述释放状态下可枢转到期望位置 。

    Mutant Cells Suitable for Recombinant Polypeptide Production
    6.
    发明申请
    Mutant Cells Suitable for Recombinant Polypeptide Production 审中-公开
    适用于重组多肽生产的突变细胞

    公开(公告)号:US20100173286A1

    公开(公告)日:2010-07-08

    申请号:US11993525

    申请日:2006-06-20

    CPC classification number: C12N9/0006 C12N1/32

    Abstract: A mutated bacterial cell producing at least one heterologous polypeptide of interest, wherein said cell has a reduced expression-level of YugJ (SEQ ID NO: 2) or a homologue thereof when compared with an otherwise isogenic but non-mutated cell; methods for producing said cell, and methods for producing a polypeptide of interest using said cell.

    Abstract translation: 产生至少一种感兴趣的异源多肽的突变的细菌细胞,其中当与其它同基因但未突变的细胞相比时,所述细胞具有降低的YugJ(SEQ ID NO:2)的表达水平或其同源物; 用于生产所述细胞的方法,以及使用所述细胞产生感兴趣的多肽的方法。

    DNase Expression in Recombinant Host Cells
    7.
    发明申请
    DNase Expression in Recombinant Host Cells 有权
    DNase在重组宿主细胞中的表达

    公开(公告)号:US20100075376A1

    公开(公告)日:2010-03-25

    申请号:US12516720

    申请日:2007-11-30

    CPC classification number: C12N1/08 C12N9/22 C12N15/75

    Abstract: The present invention relates to cells producing at least one polypeptide of interest and expressing one or more recombinant nuclease encoding gene(s) thereby producing the nuclease(s), and methods for producing a polypeptide of interest essentially free from contaminating DNA, said method comprising the steps of: (a) cultivating a cell that produces at least one polypeptide of interest and expresses one or more recombinant nuclease encoding gene(s) thereby producing the nuclease(s); and (b) isolating the polypeptide of interest.

    Abstract translation: 本发明涉及产生感兴趣的至少一种目的多肽并表达一种或多种重组核酸酶编码基因从而产生核酸酶的细胞,以及用于生产基本上不含污染DNA的目标多肽的方法,所述方法包括 步骤:(a)培养产生至少一种目的多肽的细胞,并表达一种或多种重组核酸酶编码基因从而产生核酸酶; 和(b)分离感兴趣的多肽。

    Vehicle door operating mechanism
    8.
    发明授权
    Vehicle door operating mechanism 失效
    车门操作机构

    公开(公告)号:US06792717B2

    公开(公告)日:2004-09-21

    申请号:US10440803

    申请日:2003-05-19

    Abstract: A sliding door operating mechanism for a vehicle to provide access to the vehicle through an opening in a wall thereof, includes at least one sliding door and a reciprocating power mechanism and a transmission mechanism for transmitting movement of power to the door for performing opening, closing and locking movements thereof. A beam structure carries the door, the power mechanism and the transmission mechanism. The beam structure extends across and is accommodated within an upper portion of the opening so as to be slidingly movably driven by the power mechanism between a relatively retracted position within the opening, in which the door is located in a first plane in a closed position confirmed within the opening, and a relatively less retracted position, in which the door is located in a second plane exteriorly of the opening allowing movement of the door along the second plane.

    Abstract translation: 一种用于车辆的滑动门操作机构,其通过其壁中的开口提供对车辆的通路,包括至少一个滑动门和往复动力机构,以及用于将动力传递到门以进行打开,关闭的传动机构 及其锁定运动。 梁结构承载门,动力机构和传动机构。 梁结构延伸穿过并容纳在开口的上部中,以便在开口内的相对缩回位置之间被动力机构可滑动地移动地驱动,其中门位于处于关闭位置的第一平面中 在开口内,以及相对较少的缩回位置,其中门位于开口外部的第二平面中,允许门沿第二平面移动。

    REDUCTION OF CULTURE VISCOSITY BY MANGANESE ADDITION
    9.
    发明申请
    REDUCTION OF CULTURE VISCOSITY BY MANGANESE ADDITION 审中-公开
    减少文化粘性增加

    公开(公告)号:US20140199752A1

    公开(公告)日:2014-07-17

    申请号:US14238110

    申请日:2012-08-03

    CPC classification number: C12N9/2417 C12P21/00

    Abstract: A method of producing an enzyme of interest in a fed-batch cultivation comprising: a) cultivating a microorganism in a culture medium conducive to its growth wherein the microorganism produces the enzyme of interest; and b) adding a manganese compound to the culture medium one or more times during the cultivation.

    Abstract translation: 在补料分批培养中生产感兴趣的酶的方法,其包括:a)在有助于其生长的培养基中培养微生物,其中微生物产生感兴趣的酶; 和b)在培养过程中一次或多次地向培养基中加入锰化合物。

    Ball joint device
    10.
    发明授权
    Ball joint device 有权
    球接头装置

    公开(公告)号:US07993069B2

    公开(公告)日:2011-08-09

    申请号:US12276181

    申请日:2008-11-21

    Applicant: Martin Persson

    Inventor: Martin Persson

    Abstract: A double ball joint device comprises a housing, a first joint element having a first ball mounted in the housing, and a first shaft attached to a portion of the first ball protruding through an opening in the housing, and a second joint element having a second ball, which is mounted similar to the first ball and is provided with a corresponding second shaft. A locking washer is arranged between the first and second balls and has a first seat for receiving the first ball, and an opposite second seat for receiving the second ball. An operation member is operable between a released state, where the joint elements are pivotable, and a locked state, where it urges the locking washer to clamp lock the joint elements in a current position. When moving the operation member to the locked state it urges the washer to an off-centre position.

    Abstract translation: 双球接头装置包括壳体,具有安装在壳体中的第一球的第一接头元件和附接到通过壳体中的开口突出的第一球的部分的第一轴,以及具有第二接头元件的第二接头元件, 球,其安装类似于第一球,并且设置有相应的第二轴。 锁定垫圈布置在第一和第二球之间并且具有用于接收第一球的第一座位和用于接收第二球的相对的第二座椅。 操作构件可以在关节元件可枢转的释放状态和锁定状态之间操作,其中它促使锁定垫圈将接合元件夹紧在当前位置。 当将操作构件移动到锁定状态时,它将垫圈推向偏心位置。

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