Abstract:
The present invention relates to cells producing at least one polypeptide of interest and expressing one or more recombinant nuclease encoding gene(s) thereby producing the nuclease(s), and methods for producing a polypeptide of interest essentially free from contaminating DNA, said method comprising the steps of: (a) cultivating a cell that produces at least one polypeptide of interest and expresses one or more recombinant nuclease encoding gene(s) thereby producing the nuclease(s); and (b) isolating the polypeptide of interest.
Abstract:
The present invention relates to cells producing at least one polypeptide of interest and expressing one or more recombinant nuclease encoding gene(s) thereby producing the nuclease(s), and methods for producing a polypeptide of interest essentially free from contaminating DNA, said method comprising the steps of: (a) cultivating a cell that produces at least one polypeptide of interest and expresses one or more recombinant nuclease encoding gene(s) thereby producing the nuclease(s); and (b) isolating the polypeptide of interest.
Abstract:
Subject matter of the invention is a device for an extendable reception of a ramp, wherein the device can be mounted at a vehicle in the area of the vehicle door, wherein the device comprises a drive assembly for the ramp, wherein the drive assembly comprises a slide, wherein the slide is connected with the ramp, wherein the drive equipment can be engaged with at least one power transmission element mounted at the vehicle.
Abstract:
The present invention relates to cells producing at least one polypeptide of interest and expressing one or more recombinant nuclease encoding gene(s) thereby producing the nuclease(s), and methods for producing a polypeptide of interest essentially free from contaminating DNA, said method comprising the steps of: (a) cultivating a cell that produces at least one polypeptide of interest and expresses one or more recombinant nuclease encoding gene(s) thereby producing the nuclease(s); and (b) isolating the polypeptide of interest.
Abstract:
This invention relates to a double ball joint device comprising: a housing; a first joint element comprising a first ball, wherein a major portion of said first ball is mounted and retained within said housing and a minor portion of the first ball protrudes from said housing through a first opening thereof, and a first shaft, which is attached to the first ball at said minor portion thereof; a second joint element comprising a second ball, wherein a major portion of said second ball is mounted and retained within said housing and a minor portion of the second ball protrudes from said housing through a second opening thereof, and a second shaft, which is attached to the second ball at said minor portion thereof; a locking washer, which is arranged between said first and second balls and which comprises a first seat for receiving said first ball, and an opposite second seat for receiving said second ball, and an operation member, which is operable between a locked state where it urges said locking washer to clamp lock said first and second joint elements in a current position, and a released state where it releases said locking washer thereby releasing the joint elements, wherein the joint elements are pivotable, at said released state, into a desired position.
Abstract:
A mutated bacterial cell producing at least one heterologous polypeptide of interest, wherein said cell has a reduced expression-level of YugJ (SEQ ID NO: 2) or a homologue thereof when compared with an otherwise isogenic but non-mutated cell; methods for producing said cell, and methods for producing a polypeptide of interest using said cell.
Abstract translation:产生至少一种感兴趣的异源多肽的突变的细菌细胞,其中当与其它同基因但未突变的细胞相比时,所述细胞具有降低的YugJ(SEQ ID NO:2)的表达水平或其同源物; 用于生产所述细胞的方法,以及使用所述细胞产生感兴趣的多肽的方法。
Abstract:
The present invention relates to cells producing at least one polypeptide of interest and expressing one or more recombinant nuclease encoding gene(s) thereby producing the nuclease(s), and methods for producing a polypeptide of interest essentially free from contaminating DNA, said method comprising the steps of: (a) cultivating a cell that produces at least one polypeptide of interest and expresses one or more recombinant nuclease encoding gene(s) thereby producing the nuclease(s); and (b) isolating the polypeptide of interest.
Abstract:
A sliding door operating mechanism for a vehicle to provide access to the vehicle through an opening in a wall thereof, includes at least one sliding door and a reciprocating power mechanism and a transmission mechanism for transmitting movement of power to the door for performing opening, closing and locking movements thereof. A beam structure carries the door, the power mechanism and the transmission mechanism. The beam structure extends across and is accommodated within an upper portion of the opening so as to be slidingly movably driven by the power mechanism between a relatively retracted position within the opening, in which the door is located in a first plane in a closed position confirmed within the opening, and a relatively less retracted position, in which the door is located in a second plane exteriorly of the opening allowing movement of the door along the second plane.
Abstract:
A method of producing an enzyme of interest in a fed-batch cultivation comprising: a) cultivating a microorganism in a culture medium conducive to its growth wherein the microorganism produces the enzyme of interest; and b) adding a manganese compound to the culture medium one or more times during the cultivation.
Abstract:
A double ball joint device comprises a housing, a first joint element having a first ball mounted in the housing, and a first shaft attached to a portion of the first ball protruding through an opening in the housing, and a second joint element having a second ball, which is mounted similar to the first ball and is provided with a corresponding second shaft. A locking washer is arranged between the first and second balls and has a first seat for receiving the first ball, and an opposite second seat for receiving the second ball. An operation member is operable between a released state, where the joint elements are pivotable, and a locked state, where it urges the locking washer to clamp lock the joint elements in a current position. When moving the operation member to the locked state it urges the washer to an off-centre position.