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公开(公告)号：US08524506B2
公开(公告)日：2013-09-03
申请号：US11936289
申请日：2007-11-07
Applicant: Vamsee K. Pamula , Michael G. Pollack , Richard B. Fair
Inventor： Vamsee K. Pamula , Michael G. Pollack , Richard B. Fair
IPC: G01N1/10
CPC classification number: B01F13/0071 , B01F13/0076 , B01F13/0084 , B01J2219/00479 , B01J2219/00653 , B01L3/502792 , B01L2300/089 , B01L2300/165 , B01L2400/0415 , B01L2400/0427 , C40B60/14 , G01N15/14 , G01N2035/1046 , G02B26/005 , Y10T436/11 , Y10T436/113332 , Y10T436/117497 , Y10T436/118339 , Y10T436/25 , Y10T436/25625 , Y10T436/2575
Abstract: Methods for sampling a liquid flow are provided. The method includes supplying a continuous liquid flow from a continuous flow fluid input source to a surface along a first flow path. The method additionally includes sampling the continuous liquid flow by performing an electric field-based technique to split off a sample droplet from a portion of the liquid flow, whereby the sample droplet is distinct from the liquid flow and controllable independently of the liquid flow, wherein the electric field-based technique is performed by providing a set of electrodes on the surface and selectively biasing the set of electrodes, whereby the sample droplet is formed on one of the set of electrodes.
Abstract translation: 提供了液体取样方法。 该方法包括将连续的液体流从连续流体流体输入源提供到沿着第一流动路径的表面。 该方法另外包括通过执行基于电场的技术对液体流的一部分分离样品液滴来对连续液体流进行采样，由此样品液滴与液体流不同，并且可独立于液体流动而控制，其中 通过在表面上设置一组电极并选择性地偏置该组电极来执行基于电场的技术，由此在一组电极中形成样品液滴。
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公开(公告)号：US20130146455A1
公开(公告)日：2013-06-13
申请号：US13759416
申请日：2013-02-05
Applicant: Vamsee K. Pamula , Michael G. Pollack , Richard B. Fair
Inventor： Vamsee K. Pamula , Michael G. Pollack , Richard B. Fair
IPC: C25B9/00
CPC classification number: B01L3/50273 , B01F11/0071 , B01F13/0071 , B01F13/0076 , B01F13/0084 , B01L3/00 , B01L3/5027 , B01L3/502784 , B01L3/502792 , B01L2200/06 , B01L2200/0605 , B01L2200/10 , B01L2300/089 , B01L2300/161 , B01L2300/165 , B01L2400/0415 , B01L2400/0427 , B41J2002/14395 , C25B9/00 , G01N27/44791 , G02B26/005
Abstract: Methods are provided for manipulating droplets. The methods include providing the droplet on a surface comprising an array of electrodes and a substantially co-planer array of reference elements, wherein the droplet is disposed on a first one of the electrodes, and the droplet at least partially overlaps a second one of the electrodes and an intervening one of the reference elements disposed between the first and second electrodes. The methods further include activating the first and second electrodes to spread at least a portion of the droplet across the second electrode and deactivating the first electrode to move the droplet from the first electrode to the second electrode.
Abstract translation: 提供了用于操纵液滴的方法。 所述方法包括在包括电极阵列和基本上共平面的参考元件阵列的表面上提供液滴，其中液滴设置在电极的第一个上，并且液滴至少部分地与第二个 电极和设置在第一和第二电极之间的参考元件中的插入件之一。 所述方法还包括激活第一和第二电极以将液滴的至少一部分扩散穿过第二电极，并且去激活第一电极以使液滴从第一电极移动到第二电极。
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公开(公告)号：US08048628B2
公开(公告)日：2011-11-01
申请号：US11753318
申请日：2007-05-24
Applicant: Michael G. Pollack , Vamsee K. Pamula , Richard B. Fair
Inventor： Michael G. Pollack , Vamsee K. Pamula , Richard B. Fair
CPC classification number: G01N27/44769 , B01F13/0071 , B01F13/0076 , B01L3/502707 , B01L3/502792 , B01L7/52 , B01L2200/0673 , B01L2200/10 , B01L2200/12 , B01L2300/0645 , B01L2300/0663 , B01L2300/0819 , B01L2300/0864 , B01L2300/0867 , B01L2300/0887 , B01L2300/089 , B01L2300/12 , B01L2300/1827 , B01L2400/0415 , B01L2400/0424 , B81B3/0021 , B81B2201/058 , B81C1/00119 , C12Q1/6846 , G01N27/447 , G01N27/44791 , G05D7/0694 , H01L21/6715 , Y10T436/2575
Abstract: Methods for amplifying nucleic acid, such as DNA or RNA, on a printed circuit board (PCB) substrate are disclosed. The amplification can be achieved through a variety of methods such as thermocycling or isothermally. The printed circuit board substrate can comprise an array of electrodes for transporting droplets and can be part of a sandwich structure including a top plate parallel to the printed circuit board substrate.
Abstract translation: 公开了在印刷电路板（PCB）衬底上放大核酸如DNA或RNA的方法。 扩增可以通过各种方法如热循环或等温进行。 印刷电路板基板可以包括用于输送液滴的电极阵列，并且可以是包括平行于印刷电路板基板的顶板的夹层结构的一部分。
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公开(公告)号：US07759132B2
公开(公告)日：2010-07-20
申请号：US11585491
申请日：2006-10-23
Applicant: Michael G. Pollack , Vamsee K. Pamula , Richard B. Fair
Inventor： Michael G. Pollack , Vamsee K. Pamula , Richard B. Fair
CPC classification number: B01F13/0071 , B01F13/0076 , B01F13/0084 , B01J2219/00479 , B01J2219/00653 , B01L3/502792 , B01L2300/089 , B01L2300/165 , B01L2400/0415 , B01L2400/0427 , C40B60/14 , G01N15/14 , G01N2035/1046 , G02B26/005 , Y10T436/11 , Y10T436/113332 , Y10T436/117497 , Y10T436/118339 , Y10T436/25 , Y10T436/25625 , Y10T436/2575
Abstract: Methods for performing microfluidic sampling are provided. The method includes providing a substrate including an arrangement of first, second and third electrodes, wherein the second electrode is interposed between the first and third electrodes. The method additionally includes causing a fluid input to continuously flow to the first electrode and biasing the first, second and third electrodes to a first voltage to cause a portion of the fluid input to spread across the second and third electrodes. The method further includes biasing the second electrode to a second voltage different from the first voltage to form a droplet on the third electrode, the droplet being separate from the fluid input.
Abstract translation: 提供了执行微流体取样的方法。 该方法包括提供包括第一，第二和第三电极的布置的基板，其中第二电极介于第一和第三电极之间。 该方法还包括使流体输入连续地流动到第一电极并且将第一，第二和第三电极偏压到第一电压以使流体输入的一部分扩展穿过第二和第三电极。 该方法还包括将第二电极偏压到不同于第一电压的第二电压，以在第三电极上形成液滴，液滴与流体输入分离。
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5.发明授权公开(公告)号：US06911132B2
公开(公告)日：2005-06-28
申请号：US10253368
申请日：2002-09-24
Applicant: Vamsee K. Pamula , Michael G. Pollack , Philip Y. Paik , Hong Ren , Richard B. Fair
Inventor： Vamsee K. Pamula , Michael G. Pollack , Philip Y. Paik , Hong Ren , Richard B. Fair
IPC: B01F11/00 , B01F13/00 , B01L3/00 , G01N1/00 , G01N1/10 , G01N15/06 , G01N21/00 , G01N35/00 , G01N27/26 , B01D57/00
CPC classification number: B01L3/50273 , B01F11/0071 , B01F13/0071 , B01F13/0076 , B01F13/0084 , B01L3/00 , B01L3/5027 , B01L3/502784 , B01L3/502792 , B01L2200/06 , B01L2200/0605 , B01L2200/10 , B01L2300/089 , B01L2300/161 , B01L2300/165 , B01L2400/0415 , B01L2400/0427 , B41J2002/14395 , C25B9/00 , G01N27/44791 , G02B26/005
Abstract: An apparatus is provided for manipulating droplets. The apparatus is a single-sided electrode design in which all conductive elements are contained on one surface on which droplets are manipulated. An additional surface can be provided parallel with the first surface for the purpose of containing the droplets to be manipulated. Droplets are manipulated by performing electrowetting-based techniques in which electrodes contained on or embedded in the first surface are sequentially energized and de-energized in a controlled manner. The apparatus enables a number of droplet manipulation processes, including merging and mixing two droplets together, splitting a droplet into two or more droplets, sampling a continuous liquid flow by forming from the flow individually controllable droplets, and iterative binary or digital mixing of droplets to obtain a desired mixing ratio.
Abstract translation: 提供了一种用于操纵液滴的装置。 该装置是单面电极设计，其中所有导电元件都包含在其上操作液滴的一个表面上。 为了容纳待操作的液滴，可以与第一表面平行地设置另外的表面。 通过执行基于电润湿的技术来操纵液滴，其中包含在或嵌入在第一表面中的电极被顺序地通电并以受控的方式断电。 该装置能够进行多个液滴操纵过程，包括将两个液滴合并和混合在一起，将液滴分成两个或更多个液滴，通过从流动单独控制的液滴形成连续的液体流，以及液滴的迭代二进制或数字混合到 获得所需的混合比。
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Patent Agency Ranking
公开(公告)号：US08980198B2
公开(公告)日：2015-03-17
申请号：US11639594
申请日：2006-12-15
Applicant: Vijay Srinivasan , Michael G. Pollack , Philip Y. Paik , Vamsee K. Pamula , Richard B. Fair
Inventor： Vijay Srinivasan , Michael G. Pollack , Philip Y. Paik , Vamsee K. Pamula , Richard B. Fair
IPC: B01L3/00 , G01N27/447 , B01F13/00 , B01L7/00 , G01N35/10
CPC classification number: B01L3/502792 , B01F13/0071 , B01F13/0076 , B01L3/502715 , B01L3/50273 , B01L3/502761 , B01L3/502784 , B01L7/52 , B01L7/525 , B01L7/54 , B01L2200/0647 , B01L2200/0673 , B01L2200/10 , B01L2200/147 , B01L2300/0816 , B01L2300/0861 , B01L2300/089 , B01L2300/1827 , B01L2400/0427 , B01L2400/0448 , B03C5/02 , B81B1/00 , G01N27/447 , G01N2035/1046
Abstract: The present invention relates to filler fluids for droplet operations. According to one embodiment of this aspect, a droplet microactuator is provided and includes: (a) a first substrate comprising electrodes configured for conducting droplet operations on a surface of the substrate; (b) a second substrate spaced from the surface of the substrate by a distance sufficient to define an interior volume between the first substrate and second substrate, wherein the distance is sufficient to contain a droplet disposed in the space on the first substrate; and (c) a droplet arranged in the interior volume and arranged with respect to the electrodes in a manner which permits droplet operations to be effected on the droplet using the electrodes.
Abstract translation: 本发明涉及用于液滴操作的填充流体。 根据该方面的一个实施例，提供了液滴微致动器，其包括：（a）第一基板，其包括被配置用于在所述基板的表面上进行液滴操作的电极; （b）第二衬底，其与衬底的表面间隔足以限定第一衬底和第二衬底之间的内部空间的距离，其中所述距离足以容纳设置在第一衬底上的空间中的液滴; 和（c）布置在内部体积中并且以允许使用电极对液滴进行液滴操作的方式相对于电极布置的液滴。
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公开(公告)号：US08906627B2
公开(公告)日：2014-12-09
申请号：US13737433
申请日：2013-01-09
Applicant: Michael G Pollack , Vamsee K Pamula , Richard B Fair
Inventor： Michael G Pollack , Vamsee K Pamula , Richard B Fair
IPC: C12Q1/68 , G01N27/447 , B01F13/00 , B01L3/00 , B81B3/00 , B81C1/00 , G05D7/06 , H01L21/67 , B01L7/00
CPC classification number: G01N27/44769 , B01F13/0071 , B01F13/0076 , B01L3/502707 , B01L3/502792 , B01L7/52 , B01L2200/0673 , B01L2200/10 , B01L2200/12 , B01L2300/0645 , B01L2300/0663 , B01L2300/0819 , B01L2300/0864 , B01L2300/0867 , B01L2300/0887 , B01L2300/089 , B01L2300/12 , B01L2300/1827 , B01L2400/0415 , B01L2400/0424 , B81B3/0021 , B81B2201/058 , B81C1/00119 , C12Q1/6846 , G01N27/447 , G01N27/44791 , G05D7/0694 , H01L21/6715 , Y10T436/2575
Abstract: Apparatuses and methods for manipulating droplets are disclosed. In one embodiment, an apparatus for manipulating droplets is provided, the apparatus including a substrate, multiple arrays of electrodes disposed on the substrate, wherein corresponding electrodes in each array are connected to a common electrical signal, and a dielectric layer disposed on the substrate first side surface and patterned to cover the electrodes.
Abstract translation: 公开了用于操纵液滴的装置和方法。 在一个实施例中，提供了一种用于操纵液滴的装置，该装置包括一个基板，设置在该基板上的多个电极阵列，其中每个阵列中的对应电极连接到公共电信号，以及首先设置在基板上的电介质层 侧表面并图案化以覆盖电极。
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公开(公告)号：US08492168B2
公开(公告)日：2013-07-23
申请号：US11639663
申请日：2006-12-15
Applicant: Vijay Srinivasan , Vamsee K. Pamula , Michael G. Pollack , Richard B. Fair
Inventor： Vijay Srinivasan , Vamsee K. Pamula , Michael G. Pollack , Richard B. Fair
IPC: G01N33/553
CPC classification number: G01N33/54373 , B01F13/0071 , B01F13/0076 , B01L3/0268 , B01L3/502761 , B01L3/502784 , B01L7/525 , B01L2200/027 , B01L2200/0668 , B01L2200/0673 , B01L2200/148 , B01L2300/0654 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0421 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , B01L2400/0448 , G01N15/1484 , G01N2015/1006
Abstract: The present invention relates to droplet-based affinity assays. According to one embodiment, a method of detecting a target analyte in a sample is provided, wherein the method includes: (a) executing droplet operations to combine affinity-based assay reagents on a droplet microactuator with a sample potentially comprising the target analyte to generate a signal indicative of the presence, absence and/or quantity of analyte; and (b) detecting the signal, wherein the signal corresponds to the presence, absence and/or quantity of the analyte in the sample.
Abstract translation: 本发明涉及基于液滴的亲和力测定。 根据一个实施方案，提供了一种检测样品中的目标分析物的方法，其中所述方法包括：（a）执行液滴操作，以将基于亲和性的测定试剂与液滴微致动器与潜在地包含目标分析物的样品组合以产生 指示分析物的存在，不存在和/或数量的信号; 和（b）检测信号，其中信号对应于样品中分析物的存在，不存在和/或数量。
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公开(公告)号：US08470606B2
公开(公告)日：2013-06-25
申请号：US12761147
申请日：2010-04-15
Applicant: Vijay Srinivasan , Vamsee K. Pamula , Michael G. Pollack , Richard B. Fair
Inventor： Vijay Srinivasan , Vamsee K. Pamula , Michael G. Pollack , Richard B. Fair
CPC classification number: B01F11/0071 , B01F13/0071 , B01F13/0076 , B01L3/502746 , B01L3/502761 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0421 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , G01N35/0098 , G01N2035/1046 , Y10T436/11 , Y10T436/25 , Y10T436/25625 , Y10T436/2575
Abstract: The invention provides a method of circulating magnetically responsive beads within a droplet in a droplet actuator. The invention also provides methods for splitting droplets. The invention, in one embodiment, makes use of a droplet actuator with top and bottom substrates, a plurality of magnetic fields respectively present proximate the top and bottom substrates, wherein at least one of the magnet fields is selectively alterable, and a plurality of droplet operations electrodes positioned along at least one of the top and bottom surfaces. A droplet is positioned between the top and bottom surfaces and at least one of the magnetic fields is selectively altered.
Abstract translation: 本发明提供了一种在液滴致动器中的液滴内循环磁响应珠的方法。 本发明还提供了分裂液滴的方法。 在一个实施例中，本发明利用具有顶部和底部基底的液滴致动器，分别存在于顶部和底部基底附近的多个磁场，其中至少一个磁场可选择性地改变，并且多个液滴 沿着顶表面和底表面中的至少一个定位的操作电极。 液滴位于顶表面和底表面之间，并且至少一个磁场被选择性地改变。
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公开(公告)号：US08287711B2
公开(公告)日：2012-10-16
申请号：US11965206
申请日：2007-12-27
Applicant: Michael G. Pollack , Vamsee K. Pamula , Richard B. Fair
Inventor： Michael G. Pollack , Vamsee K. Pamula , Richard B. Fair
IPC: F04B19/00
CPC classification number: B01L3/50273 , B01F11/0071 , B01F13/0071 , B01F13/0076 , B01F13/0084 , B01L3/00 , B01L3/5027 , B01L3/502784 , B01L3/502792 , B01L2200/06 , B01L2200/0605 , B01L2200/10 , B01L2300/089 , B01L2300/161 , B01L2300/165 , B01L2400/0415 , B01L2400/0427 , B41J2002/14395 , C25B9/00 , G01N27/44791 , G02B26/005
Abstract: An apparatus for manipulating droplets is provided. In one embodiment, the apparatus includes a substrate having a set of electrical leads for connecting electrodes to a controller, a first set of electrodes, each connected to a separate one of the electrical leads, and a second set of electrodes, all connected to a single one of the electrical leads. In another embodiment, the apparatus includes a substrate having a set of X electrodes, and a set of Y electrical leads, each connected to one or more electrodes, wherein X is greater than Y.
Abstract translation: 提供了一种用于操纵液滴的装置。 在一个实施例中，该装置包括具有用于将电极连接到控制器的一组电引线的基板，分别连接到电引线的单独电极的第一组电极和全部连接到 单一电引线。 在另一个实施例中，该装置包括具有一组X电极的基板和一组Y电引线，每组连接到一个或多个电极，其中X大于Y.
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