公开(公告)号：US20170234902A1

公开(公告)日：2017-08-17

申请号：US15502846

申请日：2015-08-13

Applicant: SENSIQ TECHNOLOGIES, INC.

Inventor： Christopher Iver Strande

IPC: G01N35/10 ,  G01N21/11 ,  G01N21/552

Abstract: A fluid delivery system and process for fluid delivery is provided that is useful in in flow-injection based sensing measurements, wherein samples from a sample rack are introduced into a flow cell where sensing measurements are taken. The system and process utilize at least two holding lines where each holding line can retain one of the samples prior to injection of the thus retained sample into a flow cell. The introduction of the samples from the sample racks to the holding lines is alternated and the injection of the samples from the holding lines to the flow cell are alternated so that one holding line is loaded with one of the samples simultaneously with another sample being injected from the another holding line to the flow cell.

公开(公告)号：US20180195954A1

公开(公告)日：2018-07-12

申请号：US15737658

申请日：2016-07-11

Applicant: SENSIQ TECHNOLOGIES, INC.

Inventor： Christopher Iver Strande ,  Aaron David Martin

IPC: G01N21/27 ,  G01N33/543 ,  G01N33/53 ,  G01N21/552 ,  G01N35/08

CPC classification number: G01N21/272 ,  B01L2300/0636 ,  G01N21/553 ,  G01N33/5306 ,  G01N33/54306 ,  G01N35/08 ,  G01N2021/0346 ,  G01N2021/058 ,  G01N2201/128

Abstract: Disclosed is a method for preparing dispersion gradients and an SPR injection method for determining full kinetics and affinity analysis in the presence of a competitor molecule. The SPR injection provides a dispersion gradient of two or more samples to a SPR flow cell and detector.

公开(公告)号：US09990464B1

公开(公告)日：2018-06-05

申请号：US14049863

申请日：2013-10-09

Applicant: SENSIQ TECHNOLOGIES, INC.

Inventor： John Gerard Quinn

IPC: C12M1/34 ,  G06F19/12 ,  G01N33/557 ,  G01N35/08 ,  G01N33/53 ,  G01N21/55 ,  G01N21/552 ,  G01N35/10 ,  G01N21/05

CPC classification number: G06F19/12 ,  B01L2300/0636 ,  G01N21/272 ,  G01N21/55 ,  G01N21/553 ,  G01N33/53 ,  G01N35/08 ,  G01N35/085 ,  G01N35/1097 ,  G01N2021/058 ,  G01N2035/00702

Abstract: Dispersion injection methods for determining biomolecular interaction parameters in label-free biosensing systems are provided. The methods generally relate to the use of a single analyte injection that generates a smoothly-varying concentration gradient via dispersion en route to a sensing region possessing an immobilized binding partner. The present method incorporates the use of an internal standard which provides a reference as to the dispersion conditions present which can then be used to calculate an effective diffusion coefficient for the analyte of interest based on a universal calibration function. The effective diffusion coefficient can then be incorporated into the appropriate dispersion model to provide a calibrated dispersion model. The calibrated dispersion model can then be incorporated into the desired interaction model to provide a reliable representation of the analyte concentration at the sensing region at any time during the injection. The use of the internal standard and universal calibration function permit use of a wide range of injection conditions which may not otherwise be consistent with a particular dispersion model. Thus, the present methods allow for higher flow rates and lower sample volumes thereby increasing assay speed and decreasing sample consumption.