公开(公告)号：US20070178532A1

公开(公告)日：2007-08-02

申请号：US10551126

申请日：2004-03-24

Applicant: Steven Jacobson ,  Utano Takeuchi ,  Yoshihisa Yamano

Inventor： Steven Jacobson ,  Utano Takeuchi ,  Yoshihisa Yamano

IPC: G01N33/567 ,  C07H21/04 ,  C12P21/06 ,  C12N5/08 ,  C07K14/435

CPC classification number: G01N33/56977 ,  G01N33/505 ,  G01N2800/52

Abstract: Methods of quantifying and/or characterizing antigen-specific T cell populations, identifying T cell antigens and epitopes, and preparing targeted pharmaceutical compositions by (i) introducing a peptide into an antigen-presenting cell (APC) expressing a fusion protein comprising a major histocompatibility complex portion and a reporter peptide portion such that a complex forms between the fusion protein and the peptide and the peptide is displayed by the APC and (ii) contacting the APC with a population of cells, such that T cells in the population of cells that react with the peptide detectably internalize the complex; novel APCs useful in such methods; and related therapeutic and diagnostic methods.

Abstract translation: 定量和/或表征抗原特异性T细胞群体，鉴定T细胞抗原和表位的方法，以及通过（i）将肽引入表达融合蛋白的抗原呈递细胞（APC）并制备靶向药物组合物，所述抗原呈递细胞（APC）包含主要组织相容性 复合部分和报道肽部分，使得融合蛋白和肽之间形成复合物，肽显示APC，和（ii）使APC与细胞群接触，使得细胞群体中的T细胞 与肽反应可检测内在化复合物; 这种方法有用的新型APC; 以及相关的治疗和诊断方法。