公开(公告)号：US20100099177A1

公开(公告)日：2010-04-22

申请号：US12319052

申请日：2009-01-02

Applicant: Lung-Jieh Yang ,  Yu-Cheng Ou

Inventor： Lung-Jieh Yang ,  Yu-Cheng Ou

IPC: C12M1/34

CPC classification number: C12M41/14 ,  C12M23/50 ,  C12M41/36 ,  C12M41/46

Abstract: An optical monitoring subsystem includes: a differential interference contrast (DIC) optical-path apparatus having a CCD camera and enclosed in a gas-tight housing stored in an incubator and a three-dimension electric driving device operatively moving along three axes of three-dimension coordinates for conveniently observing, monitoring or photographing cells in a cell specimen placed in a concave platform as recessed in the gas-tight housing, and a control device operatively controlling the free movement of the DIC optical-path apparatus; whereby the cells may be directly observed or monitored in-situ in the incubator in a three-dimensional way.

Abstract translation: 光学监控子系统包括：具有CCD照相机的差分干涉对比度（DIC）光路设备，并封装在储存在培养箱中的气密壳体中，以及三维电驱动装置，其可操作地沿三维轴 坐标，用于方便地观察，监测或拍摄放置在凹形平台中的凹形平台中的细胞样本中的细胞凹陷在气密壳体中;以及控制装置，可操作地控制DIC光路装置的自由运动; 从而可以以三维方式在培养箱中原位直接观察或监测细胞。

公开(公告)号：US20070184086A1

公开(公告)日：2007-08-09

申请号：US11449820

申请日：2006-06-09

Applicant: Lung-Jieh Yang ,  Yu-Cheng Ou

Inventor： Lung-Jieh Yang ,  Yu-Cheng Ou

IPC: A61K35/12 ,  A61F2/02

CPC classification number: C12N5/0068 ,  C12N2533/12 ,  C12N2533/40

Abstract: This invention proposes a novel technique for fabricating a gelatin micro pattern for cell culture. The gelatin micro pattern is formed by photolithography and then crosslinked with a crosslinking agent such as glutaraldehyde. The gelatin micro pattern can be used as an excellent cell culture platform for in-vitro observations of a certain cluster of living cells or even a single living cell.

Abstract translation: 本发明提出了一种用于制造细胞培养的明胶微图案的新技术。 明胶微图案通过光刻法形成，然后与交联剂如戊二醛交联。 明胶微图案可用作一种优良的细胞培养平台，用于体外观察某些活细胞簇甚至单个活细胞。

公开(公告)号：US08921075B2

公开(公告)日：2014-12-30

申请号：US13488748

申请日：2012-06-05

Applicant: Yen-Pei Lu ,  Ming-Yu Lin ,  Yu-Sheng Lai ,  Yuh-Shyong Yang ,  Hsuen-Li Chen ,  Yu-Cheng Ou

Inventor： Yen-Pei Lu ,  Ming-Yu Lin ,  Yu-Sheng Lai ,  Yuh-Shyong Yang ,  Hsuen-Li Chen ,  Yu-Cheng Ou

IPC: C12P19/34 ,  C12Q1/68

CPC classification number: C12P19/34 ,  C12Q1/6834 ,  C12Q2525/151 ,  C12Q2525/307 ,  C12Q2563/155

Abstract: A method of manufacturing a nanoparticle chain is disclosed. The method comprises the steps of: providing a single-stranded circular primer with a determined length, and amplifying the single-stranded circular primer into single-stranded DNA nanotemplate by an isothermal nucleotide amplification reaction such that an end of the single-stranded DNA nanotemplate is fixed to a surface of a substrate; and adding a single-stranded DNA probe conjugated with nanoparticle at one end of which, and attaching the single-stranded DNA probe to the corresponding sequence on the single-stranded DNA nanotemplate to form a nanoparticles chain. The method of manufacturing a nanoparticle chain further comprises providing a fluid, and the flowing direction of the fluid controls the aligning direction of the nanoparticle chain. Wherein, the inter-nanoparticle distance of the nanoparticle chain can be adjusted by adjusting a reaction temperature or adding the single-stranded DNA probe without conjugating with nanoparticles.

Abstract translation: 公开了一种制造纳米颗粒链的方法。 该方法包括以下步骤：提供具有确定长度的单链环状引物，并通过等温核苷酸扩增反应将单链环状引物扩增成单链DNA纳米模板，使得单链DNA纳米模板的末端 固定在基板的表面上; 并在其一端加入与纳米颗粒缀合的单链DNA探针，并将单链DNA探针连接到单链DNA纳米模板上的相应序列上以形成纳米颗粒链。 制造纳米颗粒链的方法还包括提供流体，流体的流动方向控制纳米颗粒链的排列方向。 其中，可以通过调节反应温度或加入单链DNA探针而不与纳米颗粒共轭来调节纳米颗粒链的纳米颗粒间距离。

公开(公告)号：US20130273610A1

公开(公告)日：2013-10-17

申请号：US13488748

申请日：2012-06-05

Applicant: Yen-Pei Lu ,  Ming-Yu Lin ,  Yu-Sheng Lai ,  Yuh-Shyong Yang ,  Hsuen-Li Chen ,  Yu-Cheng Ou

Inventor： Yen-Pei Lu ,  Ming-Yu Lin ,  Yu-Sheng Lai ,  Yuh-Shyong Yang ,  Hsuen-Li Chen ,  Yu-Cheng Ou

IPC: C12P19/34

CPC classification number: C12P19/34 ,  C12Q1/6834 ,  C12Q2525/151 ,  C12Q2525/307 ,  C12Q2563/155

Abstract: A method of manufacturing a nanoparticle chain is disclosed. The method comprises the steps of: providing a single-stranded circular primer with a determined length, and amplifying the single-stranded circular primer into single-stranded DNA nanotemplate by an isothermal nucleotide amplification reaction such that an end of the single-stranded DNA nanotemplate is fixed to a surface of a substrate; and adding a single-stranded DNA probe conjugated with nanoparticle at one end of which, and attaching the single-stranded DNA probe to the corresponding sequence on the single-stranded DNA nanotemplate to form a nanoparticles chain. The method of manufacturing a nanoparticle chain further comprises providing a fluid, and the flowing direction of the fluid controls the aligning direction of the nanoparticle chain. Wherein, the inter-nanoparticle distance of the nanoparticle chain can be adjusted by adjusting a reaction temperature or adding the single-stranded DNA probe without conjugating with nanoparticles.

Abstract translation: 公开了一种制造纳米颗粒链的方法。 该方法包括以下步骤：提供具有确定长度的单链环状引物，并通过等温核苷酸扩增反应将单链环状引物扩增成单链DNA纳米模板，使得单链DNA纳米模板的末端 固定在基板的表面上; 并在其一端加入与纳米颗粒缀合的单链DNA探针，并将单链DNA探针连接到单链DNA纳米模板上的相应序列上以形成纳米颗粒链。 制造纳米颗粒链的方法还包括提供流体，流体的流动方向控制纳米颗粒链的排列方向。 其中，可以通过调节反应温度或加入单链DNA探针而不与纳米颗粒共轭来调节纳米颗粒链的纳米颗粒间距离。