公开(公告)号：US20110027827A1

公开(公告)日：2011-02-03

申请号：US12512337

申请日：2009-07-30

Applicant: Zhanyou Chi ,  Yubin Zheng ,  Ben Lucker ,  Shulin Chen

Inventor： Zhanyou Chi ,  Yubin Zheng ,  Ben Lucker ,  Shulin Chen

IPC: C12P1/00 ,  C12M1/00 ,  C12M1/34 ,  C12M1/38

CPC classification number: C12P7/649 ,  C12M21/02 ,  C12M23/18 ,  C12M43/02 ,  C12M43/08 ,  C12N1/12 ,  C12P7/6463 ,  Y02E50/13 ,  Y02E50/343

Abstract: Disclosed is a culture system for the production of algae biomass to obtain lipid, protein and carbohydrate. By integrating heterotrophic processes with a phototrophic process in parallel, this system provides year around production in colder climates. By integrating heterotrophic processes with a phototrophic process in series, this system creates a two-stage, separated mixed-trophic algal process that uses organic carbon and nutrients for the production of seed in the heterotrophic process, followed by release of cultured seed in large-scale phototrophic culture for cell biomass accumulation. Organic carbon source including waste materials can be used to feed the heterotrophic process. The production capacity ratio between the heterotrophic and the phototrophic processes can be adjusted according to season and according to the availability of related resources. The systems are used for producing and harvesting an algal biofuel feedstock as well as other potential high-value products. The sequence and approach enhances utilization of carbon and nutrient waste-streams, provides an effective method for controlling contamination, adds flexibility in regard to production and type of available products, and supplies greater economic viability due to maximized use of available growth surface areas.

Abstract translation: 公开了一种用于生产藻类生物质以获得脂质，蛋白质和碳水化合物的培养系统。 通过将异养过程与光合过程并行整合，该系统在较冷的气候条件下提供一年的生产。 通过将异养过程与光合作用进行串联整合，该系统创建了一个两阶段分离的混合营养藻类过程，其使用有机碳和营养物质在异养过程中产生种子，随后将大量培养的种子释放， 用于细胞生物量积累的大规模光养殖培养。 有机碳源（包括废料）可用于饲养异养过程。 异养和光养养过程之间的生产能力比可以根据季节和相关资源的可用性进行调整。 该系统用于生产和收获藻类生物燃料原料以及其他潜在的高价值产品。 序列和方法提高了碳和营养废物流的利用率，为控制污染提供了有效的方法，增加了可用产品的生产和类型的灵活性，并且由于最大限度地利用可用的生长表面积而提供了更大的经济可行性。

公开(公告)号：US20120083023A1

公开(公告)日：2012-04-05

申请号：US13225656

申请日：2011-09-06

Applicant: Xiaochen YU ,  Yubin ZHENG ,  Shulin CHEN

Inventor： Xiaochen YU ,  Yubin ZHENG ,  Shulin CHEN

IPC: C12P1/02

CPC classification number: C12P7/6409 ,  C10G2300/1014 ,  C10L1/02 ,  C10L1/026 ,  C12N1/22 ,  C12P7/649 ,  C13K1/02 ,  C13K13/002 ,  C13K13/007 ,  Y02E50/13 ,  Y02P30/20

Abstract: Oleaginous yeast strains are used to hydrolyze biomass (e.g. wheat straw) that has been pretreated using dilute acid, in order to produce lipids. The lipids may be used as feedstock for producing biofuels.

Abstract translation: 油性酵母菌株用于水解已经使用稀酸预处理的生物质（例如小麦秸秆），以产生脂质。 脂质可用作生产生物燃料的原料。

公开(公告)号：US08802409B2

公开(公告)日：2014-08-12

申请号：US13225656

申请日：2011-09-06

Applicant: Xiaochen Yu ,  Yubin Zheng ,  Shulin Chen

Inventor： Xiaochen Yu ,  Yubin Zheng ,  Shulin Chen

IPC: C12P1/02

CPC classification number: C12P7/6409 ,  C10G2300/1014 ,  C10L1/02 ,  C10L1/026 ,  C12N1/22 ,  C12P7/649 ,  C13K1/02 ,  C13K13/002 ,  C13K13/007 ,  Y02E50/13 ,  Y02P30/20

Abstract: Oleaginous yeast strains are used to hydrolyze biomass (e.g. wheat straw) that has been pretreated using dilute acid, in order to produce lipids. The lipids may be used as feedstock for producing biofuels.

Abstract translation: 油性酵母菌株用于水解已经使用稀酸预处理的生物质（例如小麦秸秆），以产生脂质。 脂质可用作生产生物燃料的原料。

公开(公告)号：US09322038B2

公开(公告)日：2016-04-26

申请号：US14130986

申请日：2012-07-06

Applicant: Xiaochen Yu ,  Jijiao Zeng ,  Yubin Zheng ,  Mahesh Bule ,  Shulin Chen

Inventor： Xiaochen Yu ,  Jijiao Zeng ,  Yubin Zheng ,  Mahesh Bule ,  Shulin Chen

IPC: C12P19/02 ,  C12P19/14 ,  C12P7/64 ,  D21C1/08

CPC classification number: C12P7/6409 ,  C12P7/6463 ,  C12P2203/00 ,  Y02E50/343

Abstract: Methods for producing lipids from lignocellulosic biomass are provided. Sugars produced by pretreatment of lignocellulosic biomass are utilized by heterotrophic oleaginous fungi or yeast to increase their biomass and to produce lipids without prior detoxification of the pretreated biomass. After the fungi/yeast are cultured with the sugars, solid residues from the pretreated biomass are combined with the fungi/yeast under conditions which allow simultaneous 1) enzymatic degradation of cellulose and/or hemicellulose to produce sugars and 2) fermentation of the sugars for further increases in biomass and lipid production.

Abstract translation: 提供了从木质纤维素生物质生产脂质的方法。 由异养油性真菌或酵母通过预处理木质纤维素生物质产生的糖增加其生物量并产生脂质，而不预先处理的生物质的解毒。 在将真菌/酵母与糖一起培养后，来自预处理的生物质的固体残余物与真菌/酵母在允许同时进行1）酶促降解纤维素和/或半纤维素以产生糖的条件下进行组合，以及2）发酵糖 生物量和脂质生产进一步增加。

公开(公告)号：US20140234919A1

公开(公告)日：2014-08-21

申请号：US14130986

申请日：2012-07-06

Applicant: Xiaochen Yu ,  Jijiao Zeng ,  Yubin Zheng ,  Mahesh Bule ,  Shulin Chen

Inventor： Xiaochen Yu ,  Jijiao Zeng ,  Yubin Zheng ,  Mahesh Bule ,  Shulin Chen

IPC: C12P7/64

CPC classification number: C12P7/6409 ,  C12P7/6463 ,  C12P2203/00 ,  Y02E50/343

Abstract: Methods and systems for producing lipids which may be used as biofuel are provided. Sugars produced by pretreatment of lignocellulosic biomass are utilized by heterotrophic oleaginous fungi or yeast to increase their biomass and to produce lipids without prior detoxification of the pretreated biomass. After the fungi/yeast are cultured with the sugars, solid residues from the pretreated biomass are combined with the fungi/yeast under conditions which allow simultaneous 1) enzymatic degradation of cellulose and/or hemicellulose to produce sugars and 2) fermentation of the sugars for further increases in biomass and lipid production.

Abstract translation: 提供了可用作生物燃料的生产脂质的方法和系统。 由异养油性真菌或酵母通过预处理木质纤维素生物质产生的糖增加其生物量并产生脂质，而不预先处理的生物质的解毒。 在将真菌/酵母与糖一起培养后，来自预处理的生物质的固体残余物与真菌/酵母在允许同时进行1）酶促降解纤维素和/或半纤维素以产生糖的条件下进行组合，以及2）发酵糖 生物量和脂质生产进一步增加。