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    BIOLOGICAL STERILIZATION INDICATOR DEVICES AND METHODS OF USE
    1.
    发明申请
    BIOLOGICAL STERILIZATION INDICATOR DEVICES AND METHODS OF USE 审中-公开
    生物灭菌指示剂装置及其使用方法

    公开(公告)号：US20150337354A1

    公开(公告)日：2015-11-26

    申请号：US14377203

    申请日：2013-02-11

    Applicant: Francois AHIMOU ,  Sailaja CHANDRAPATI ,  Kurt J. HALVERSON ,  Heather M. WEBB ,  Michael G. WILLIAMS ,  3M INNOVATIVE PROPERTIES COMPANY

    Inventor： FRANCOIS AHIMOU ,  SAILAJA CHANDRAPATI ,  KURT J. HALVERSON ,  HEATHER M. WEBB ,  MICHAEL G. WILLIAMS

    IPC: C12Q1/22 ,  G01N21/64

    CPC classification number: C12Q1/22 ,  G01N21/6428 ,  G01N2201/061

    Abstract: A biological sterilization indicator device is provided. The device comprises a body, a plurality of test microorganisms, and an oxygen-modulated first fluorescent sensor. The body comprises a first layer attached to a second layer, forming at least one isolatable microchamber and at least one primary passageway that provides fluidic communication between ambience and the at least one microchamber. The microchamber has an isolated volume of about 0.5 microliters to about 9.5 microliters. The plurality of test microorganisms and the oxygen-modulated first fluorescent sensor are disposed in the microchamber. A method of using the device to determine the effectiveness of a sterilization process is also provided.

    Abstract translation: 提供生物灭菌指示装置。 该装置包括主体，多个测试微生物和氧调制的第一荧光传感器。 主体包括附接到第二层的第一层，形成至少一个可隔离的微室和至少一个主通道，其提供环境与至少一个微室之间的流体连通。 微室具有约0.5微升至约9.5微升的隔离体积。 多个测试微生物和氧调制的第一荧光传感器设置在微室中。 还提供了使用该装置来确定灭菌过程的有效性的方法。

    METHOD OF DETECTING GAS-PRODUCING MICROBIAL COLONIES
    2.
    发明申请
    METHOD OF DETECTING GAS-PRODUCING MICROBIAL COLONIES 有权
    检测气体生产微生物群落的方法

    公开(公告)号：US20150339510A1

    公开(公告)日：2015-11-26

    申请号：US14652379

    申请日：2013-12-13

    Applicant: 3M INNOVATIVE PROPERTIES COMPANY

    Inventor： PHILLIP A. BOLEA ,  MICHAEL G. WILLIAMS

    IPC: G06K9/00 ,  G06T7/60 ,  G06T7/00

    CPC classification number: G06K9/00147 ,  G06K9/00134 ,  G06K9/0014 ,  G06T7/149 ,  G06T7/62 ,  G06T7/70 ,  G06T2207/10016 ,  G06T2207/10056 ,  G06T2207/10152 ,  G06T2207/20124 ,  G06T2207/30242

    Abstract: A first method comprises using an imaging device to produce a plurality of images of a culture device, analyzing a first image to identify a microorganism colony at a first location, analyzing a second image to identify a gas bubble at a second location, and determining whether the first location is proximate the second location. A second method comprises analyzing an image of a culture device to detect gas bubbles and classifying the gas bubbles according to a size parameter associated with each of the gas bubbles. A third method comprises analyzing a first area of an image of a culture device to detect a first number of gas bubbles, analyzing a second area of the image to detect a second number of gas bubbles, and comparing the first number of gas bubbles to the second number of gas bubbles.

    Abstract translation: 第一种方法包括使用成像装置产生培养装置的多个图像，分析第一图像以在第一位置识别微生物菌落，分析第二图像以识别第二位置处的气泡，以及确定是否 第一个位置靠近第二个位置。 第二种方法包括分析培养装置的图像以检测气泡并根据与每个气泡相关联的尺寸参数对气泡进行分类。 第三种方法包括分析培养装置的图像的第一区域以检测第一数量的气泡，分析图像的第二区域以检测第二数量的气泡，并将第一数量的气泡与 第二数量的气泡。

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