公开(公告)号：US20190391149A1

公开(公告)日：2019-12-26

申请号：US16458986

申请日：2019-07-01

Applicant: ACADEMIA SINICA

Inventor： Chi-Huey WONG ,  Chung-Yi WU ,  Chi-Hui LIANG ,  An-Suei YANG

IPC: G01N33/569 ,  G01N33/543 ,  G01N33/53

Abstract: Glycan arrays that can detect and distinguish between various sub-types and strains of influenza virus are provided. Methods for using the glycan arrays with assays using nanoparticle amplification technique are disclosed. Sandwich assays using gold nanoparticles conjugated to phage particles comprising influenza virus-specific antibodies for detecting multiple serotypes using a single reaction are provided. Plurality of glycans directed to specific target HA of influenza virus comprises the array. Detector molecules comprising noble metals conjugated to (a) phage display particles expressing antibodies against hemagglutinin and (b) neuraminidase binding agents are disclosed.

公开(公告)号：US20160166661A1

公开(公告)日：2016-06-16

申请号：US14906663

申请日：2014-07-26

Applicant: ACADEMIA SINICA

Inventor： Chi-Huey WONG ,  Chung-Yi WU ,  Hsien-Yeh HSU ,  Shih-Fen LIAO ,  Chi-Hui LIANG

IPC: A61K39/00

CPC classification number: A61K39/0002 ,  A61K36/074 ,  A61K39/0011 ,  A61K2039/55572 ,  A61K2039/575

Abstract: Immunogenic compositions, cancer vaccines and methods for treating cancer comprising FMS, the fucose-enriched polysaccharide fraction from Reishi F3, are provided. Compositions comprise fucose-enriched Reishi polysaccharide fraction (FMS) MW=˜35 kDa, wherein the FMS is isolated by size-exclusion chromatography from Reishi F3, and the FMS comprises polysaccharides having primarily a backbone selected from 1,4-mannan and 1,6-α-galactan, wherein the backbone is linked to a terminal fucose-containing side-chain Immunogenic compositions comprising glycolipid adjuvants are provided. Antibodies generated by immunogenic compositions disclosed herein bind cancer cells comprising antigens Globo H, Globo H, Gb3, Gb4, Gb5 (SSEA-3) and SSEA-4 on the cell surface.

Abstract translation: 提供免疫原性组合物，癌症疫苗和治疗癌症的方法，其包括FMS，来自Reishi F3的岩藻糖富集的多糖部分。 组合物包含岩藻糖富集的赖斯多糖级分（FMS）MW =〜35kDa，其中通过来自Reishi F3的尺寸排阻色谱法分离FMS，并且FMS包含主要选自1,4-甘露聚糖和1， 6-α-半乳聚糖，其中所述主链与包含末端岩藻糖的侧链连接，提供包含糖脂佐剂的免疫原性组合物。 由本文公开的免疫原性组合物产生的抗体结合细胞表面上包含抗原Globo H，Globo H，Gb 3，Gb 4，Gb 5（SSEA-3）和SSEA-4的癌细胞。