公开(公告)号：US20230204508A1

公开(公告)日：2023-06-29

申请号：US18084997

申请日：2022-12-20

Applicant: ACADEMIA SINICA

Inventor： Pei-Kuen WEI ,  Sheng-Hann WANG ,  Ting-Wei CHANG

IPC: G01N21/552

CPC classification number: G01N21/554 ,  G01N2021/177

Abstract: A detection method and a detection system for detecting objects of interest attached to a surface of a plurality of reporters, wherein the plurality of reporters are flowing in a microfluidic chip and illuminated by a light source. The detection method has following steps: obtaining a plurality of local surface plasmon resonance (LSPR) spectral images of each the plurality of the reporters individually, wherein each of the LSPR spectral images has a brightness of a long wavelength band (BA) and a brightness of a short wavelength band (BB); calculating a spectral image brightness contrast γ for each of the LSPR spectral images, wherein





γ
=



B
A

-

B
B




B
A

+

B
B




;




and, defining a positive threshold for |γ|≥0.1.