公开(公告)号：US20170088824A1

公开(公告)日：2017-03-30

申请号：US15302903

申请日：2015-04-09

Applicant: ADEKA CORPORATION

Inventor： Toshihiko TAKEHANA ,  Seiji KOIKE ,  Tomohisa KUZUYAMA

IPC: C12N9/04 ,  C12N15/52 ,  C12P5/00

CPC classification number: C12N9/0006 ,  C12N15/52 ,  C12P5/007 ,  C12Y101/01034 ,  C12Y101/01088

Abstract: The object of the present invention is to efficiently produce useful terpenoid compounds, and specifically, to provide a method for preparing squalene, which is an important intermediate of terpenoid. The object can be solved by a hydroxymethylglutaryl CoA reductase (HMGR) comprising:(a) an amino acid other than alanine (A) at the 10th position in an Sα2 amino acid sequence of HMGR, (b) an amino acid other than proline (P) at the 1st position from the carboxyl terminal in the DKK region of the HMG-CoA binding site of HMGR, (c) an amino acid other than alanine (A) at the 1st position in an Lα2 amino acid sequence of HMGR, and (d) an amino acid other than glutamic acid (E) at the 6th position in an Lα2 amino acid sequence of HMGR of the present invention.

公开(公告)号：US20190078120A1

公开(公告)日：2019-03-14

申请号：US16080928

申请日：2017-03-03

Applicant: NIIGATA UNIVERSITY ,  ADEKA CORPORATION

Inventor： Tsutomu SATO ,  Kotone OKUNO ,  Toshihiko TAKEHANA ,  Seiji KOIKE

IPC: C12P7/04 ,  C12N9/90 ,  C12N1/32 ,  C12N1/20 ,  C12N9/88 ,  C12N9/04

Abstract: An object of the present invention is to provide a method for preparing ambrein, which can easily obtain the ambrein. The object can be solved by a mutated tetraprenyl-β-curcumene cyclase wherein a fourth amino acid residue of a DXDD motif, aspartic acid, is substituted with an amino acid other than aspartic acid, (a) having a QXXXGX(W/F) motif, and a QXXXX(G/A)X(F/W/Y) motif on the N-terminal side, and a QXXXGX(F/W/Y) motif, and a QXXXGXW motif and a QXXXGX(F/W) motif on the C-terminal side, and not having a QXXXGXW motif at a position separated by 170 amino acid residues or more on the C-terminal side, with respect to the DXDD motif, (b) having 40% or more identity with the amino acid sequence of SEQ ID NO: 1 or SEQ ID NO: 13, (c) exhibiting ambrein production activity using squalene as a substrate.