公开(公告)号：US20230357753A1

公开(公告)日：2023-11-09

申请号：US18026264

申请日：2021-09-09

Applicant: AMGEN INC.

Inventor： Huong Thi Ngoc Le ,  Jasmine Tat ,  Ewelina Zasadzinska

IPC: C12N15/10 ,  G16B25/10 ,  C12M1/36

CPC classification number: C12N15/1034 ,  C12M41/48 ,  G16B25/10

Abstract: Methods and systems for determining a minimum number of cell line clones necessary to produce a product having a set of target product attributes are disclosed. An example method includes generating at least one cell line capable of expressing a polypeptide; measuring, using one or more analytical instruments, a plurality of measured product attribute values of a plurality of clones of a candidate cell line; receiving inputs, via a user interface, representing a set of target product attribute values for a product; projecting, by one or more processors based upon the plurality of measured values, a minimum number of subject clones of the product using the candidate cell line necessary to produce a subset of the subject clones having product attributes that satisfy one or more conditions associated with the set of target values; and generating the projected minimum number of subject clones of the product using the candidate cell line.

公开(公告)号：US20230086042A1

公开(公告)日：2023-03-23

申请号：US17909062

申请日：2021-03-04

Applicant: AMGEN INC.

Inventor： Yu Yuan ,  Tony Y. Wang ,  Kim H. Le ,  Christopher Tan ,  Jasmine Tat ,  Thorsten Dzidowski

IPC: G06T7/00

Abstract: A method of facilitating a growth assessment for a cell line includes generating an image of a well that contains a medium that was inoculated with at least one cell of the cell line. The method also includes generating a down-sampled segmentation map comprising pixels that indicate an inferred presence or absence of a cell colony in corresponding portions of the well image. Generating the down-sampled segmentation map includes inputting the well image to a fully convolutional neural network having a plurality of convolutional layers. The method also includes (i) determining, by inputting colony size information (including the down-sampled segmentation map and/or a pixel count derived therefrom) to a cell growth assessment algorithm, a growth classification or score for the cell line, and causing a display of the growth classification or score, and/or (II) causing a display of the colony size information to facilitate a manual cell growth assessment.