公开(公告)号：US20240043887A1

公开(公告)日：2024-02-08

申请号：US18268788

申请日：2021-12-24

Applicant: API CORPORATION

Inventor： Yasuhisa ASANO ,  Suguru SHINODA ,  Junichi ENOKI ,  Ryoma MIYAKE ,  Haruka SASANO

IPC: C12P17/12 ,  C12N9/02 ,  C12N15/70

CPC classification number: C12P17/12 ,  C12N9/0071 ,  C12N15/70 ,  C12Y114/11002 ,  C12R2001/19

Abstract: The present invention aims to provide a mutant L-pipecolic acid hydroxylase for highly productive and highly selective production of cis-5-hydroxy-L-pipecolic acid by hydroxylation of L-pipecolic acid, and to provide a novel method for industrially producing cis-5-hydroxy-L-pipecolic acid from L-pipecolic acid with high productivity at low cost. The present invention provides a mutant L-pipecolic acid hydroxylase having an amino acid sequence that a particular amino acid mutation(s) is/are introduced in the amino acid sequence of SEQ ID NO:2.