公开(公告)号：US09334509B2

公开(公告)日：2016-05-10

申请号：US14637429

申请日：2015-03-04

Applicant: API CORPORATION

Inventor： Hiroshi Kawabata ,  Ryoma Miyake ,  Kuniko Asada ,  Ryouhei Katou

IPC: C12P7/62 ,  C12N9/18 ,  C12P41/00 ,  C12N9/14

CPC classification number: C12P7/62 ,  C12N9/14 ,  C12N9/18 ,  C12P41/005 ,  Y02P20/52

Abstract: It is an object of the present invention to provide a novel hydrolase, which is used when dialkyl 2-vinylcyclopropane-1,1-dicarboxylate is hydrolyzed with an enzyme, so as to efficiently obtain (1S,2S)-1-alkoxycarbonyl-2-vinylcyclopropanecarboxylic acid that is useful as an intermediate for synthesizing therapeutic agents for hepatitis C. According to the present invention, there is provided a hydrolase protein, which consists of the amino acid sequence shown in any one of SEQ ID NOS. 2 to 5 and which has activity of catalyzing, at higher selectivity than the protein consisting of the amino acid sequence shown in SEQ ID NO. 1, a reaction of producing (1S,2S)-1-ethoxycarbonyl-2-vinylcyclopropanecarboxylic acid from diethyl 2-vinylcyclopropane-1,1 -dicarboxylate.

Abstract translation: 本发明的一个目的是提供一种新的水解酶，其在2-乙烯基环丙烷-1,1-二羧酸二烷基酯用酶水解时使用，以有效地获得（1S，2S）-1-烷氧基羰基-2 - 乙烯基环丙烷羧酸，其可用作合成丙型肝炎治疗剂的中间体。根据本发明，提供了水解酶蛋白质，其由SEQ ID NOS中任一项所示的氨基酸序列组成。 2至5，并且其具有以比由SEQ ID NO。所示的氨基酸序列组成的蛋白质更高的选择性催化的活性。 1，从2-乙烯基环丙烷-1,1-二羧酸二乙酯生产（1S，2S）-1-乙氧基羰基-2-乙烯基环丙烷甲酸的反应。