公开(公告)号：US20130115611A1

公开(公告)日：2013-05-09

申请号：US13673574

申请日：2012-11-09

Applicant: Applied Biosystems, LLC

Inventor： Stephen J. Gunstream

IPC: G01N21/64

CPC classification number: C12Q1/6851 ,  G01N21/274 ,  G01N21/6428 ,  G01N21/6486 ,  G01N2021/6439 ,  G01N2021/6441 ,  G01N2201/12 ,  G01N2201/12746 ,  G16B30/00 ,  C12Q2545/113 ,  C12Q2545/101

Abstract: The present teachings relate to a method of generating calibration information during a real-time polymerase chain reaction (RT-PCR) or other amplification reaction. A sample well plate or other support can contain one or more dyes or other reference materials that are subjected to the same RT-PCR thermal cycles or other conditions used to conduct amplification or other reactions on a biological sample. A set of maxima values and a set of minimum values, and/or other calibration information useful for adjusting emission data for sample dyes can be recorded, for example, for 10 cycles, 20 cycles, or each cycle of a complete RT-PCR run. Such testing of dye response under realistic operating conditions can enable more accurate characterization of plate, dye, filter, or instrument response and therefore more accurate calibration corrections and other and/or adjustments.

公开(公告)号：US20150368702A1

公开(公告)日：2015-12-24

申请号：US14750463

申请日：2015-06-25

Applicant: APPLIED BIOSYSTEMS, LLC

Inventor： Stephen J. Gunstream

IPC: C12Q1/68 ,  G01N21/64

CPC classification number: C12Q1/6851 ,  G01N21/274 ,  G01N21/6428 ,  G01N21/6486 ,  G01N2021/6439 ,  G01N2021/6441 ,  G01N2201/12 ,  G01N2201/12746 ,  G16B30/00 ,  C12Q2545/113 ,  C12Q2545/101

Abstract: The present teachings relate to a method of generating calibration information during a real-time polymerase chain reaction (RT-PCR) or other amplification reaction. A sample well plate or other support can contain one or more dyes or other reference materials that are subjected to the same RT-PCR thermal cycles or other conditions used to conduct amplification or other reactions on a biological sample. A set of maxima values and a set of minimum values, and/or other calibration information useful for adjusting emission data for sample dyes can be recorded, for example, for 10 cycles, 20 cycles, or each cycle of a complete RT-PCR run. Such testing of dye response under realistic operating conditions can enable more accurate characterization of plate, dye, filter, or instrument response and therefore more accurate calibration corrections and other and/or adjustments.

Abstract translation: 本教导涉及在实时聚合酶链反应（RT-PCR）或其他扩增反应期间产生校准信息的方法。 样品孔板或其它载体可以含有一种或多种经受相同RT-PCR热循环或用于在生物样品上进行扩增或其他反应的其他条件的染料或其它参考物质。 可以记录一组最大值和一组最小值，和/或可用于调整样品染料的发射数据的其他校准信息，例如10个循环，20个循环或完整RT-PCR运行的每个循环 。 在真实的操作条件下对染料响应的这种测试可以实现板，染料，过滤器或仪器响应的更精确的表征，因此可以进行更精确的校准校正和其他和/或调整。

公开(公告)号：US20180327826A1

公开(公告)日：2018-11-15

申请号：US15979129

申请日：2018-05-14

Applicant: APPLIED BIOSYSTEMS, LLC

Inventor： Stephen J. Gunstream

IPC: C12Q1/6851 ,  G01N21/64 ,  G06F19/22 ,  G01N21/27

CPC classification number: C12Q1/6851 ,  G01N21/274 ,  G01N21/6428 ,  G01N21/6486 ,  G01N2021/6439 ,  G01N2021/6441 ,  G01N2201/12 ,  G01N2201/12746 ,  G06F19/22 ,  C12Q2545/113 ,  C12Q2545/101

Abstract: The present teachings relate to a method of generating calibration information during a real-time polymerase chain reaction (RT-PCR) or other amplification reaction. A sample well plate or other support can contain one or more dyes or other reference materials that are subjected to the same RT-PCR thermal cycles or other conditions used to conduct amplification or other reactions on a biological sample. A set of maxima values and a set of minimum values, and/or other calibration information useful for adjusting emission data for sample dyes can be recorded, for example, for 10 cycles, 20 cycles, or each cycle of a complete RT-PCR run. Such testing of dye response under realistic operating conditions can enable more accurate characterization of plate, dye, filter, or instrument response and therefore more accurate calibration corrections and other and/or adjustments.

公开(公告)号：US20160040222A1

公开(公告)日：2016-02-11

申请号：US14798709

申请日：2015-07-14

Applicant: APPLIED BIOSYSTEMS, LLC

Inventor： Stephen J. Gunstream

IPC: C12Q1/68 ,  G06F19/22 ,  G01N21/64

CPC classification number: C12Q1/6851 ,  G01N21/274 ,  G01N21/6428 ,  G01N21/6486 ,  G01N2021/6439 ,  G01N2021/6441 ,  G01N2201/12 ,  G01N2201/12746 ,  G16B30/00 ,  C12Q2545/113 ,  C12Q2545/101

Abstract: Systems and methods are provided for calibrating emission data or other information signals collected during a polymerase chain reaction (PCR), amplification reaction, assay, process, or other reaction. Calibration of multiple detectable materials can be achieved during a single cycle or run, or during a plurality of runs of the reaction. A reading from every well, container, or other support region of a sample support does not have to be taken. Interpolation can be used to determine values for emission data or other information signals that were not taken, or are unknown, using detected emission data, or other detected information signals. By calibrating the detected emission data and the interpolated data, a more accurate reading of emission data or information signal can be obtained.

Abstract translation: 提供了系统和方法，用于校准在聚合酶链式反应（PCR），扩增反应，测定，过程或其他反应期间收集的排放数据或其他信息信号。 可以在单个循环或运行期间或在多次反应运行期间实现多个可检测材料的校准。 来自样品支架的每个孔，容器或其他支撑区域的读数不必采取。 内插可用于确定未使用或未知的使用检测到的发射数据或其他检测到的信息信号的发射数据或其他信息信号的值。 通过校准检测到的发射数据和内插数据，可以获得更准确的发射数据或信息信号读数。