公开(公告)号：US20160377620A1

公开(公告)日：2016-12-29

申请号：US15258979

申请日：2016-09-07

Applicant: ABBOTT LABORATORIES

Inventor： Brian C. Rodgers ,  Graham J. Burch

IPC: G01N33/576

CPC classification number: G01N33/5767 ,  C07K16/109 ,  G01N33/56966 ,  G01N33/56983 ,  G01N33/686 ,  G01N2333/186 ,  G01N2469/10 ,  G01N2469/20

Abstract: An in vitro method that allows detection of hepatitis C by detecting hepatitis C virus (HCV) core protein and antibodies to HCV core protein (anti-core antibodies) in a single assay is provided. Cross-reactivity is eliminated in the method preferably by utilizing short peptides, each of which has an amino acid sequence that corresponds to an immunodominant region of the native core protein but which does not wholly encompass the epitope bound by the antibodies utilized in the method. The method can be used to detect the presence of HCV in a subject, and/or to determine the suitability of donor blood or blood products for transfusion purposes. Also provided are diagnostic kits for carrying out the method and a process for selecting suitable capture peptides and monoclonal antibodies for use in the combination method.

公开(公告)号：US09470686B2

公开(公告)日：2016-10-18

申请号：US14480978

申请日：2014-09-09

Applicant: Abbott Laboratories

Inventor： Brian C. Rodgers ,  Graham J. Burch

IPC: C07K14/005 ,  C12Q1/70 ,  G01N33/569 ,  C07K16/10 ,  G01N33/576 ,  G01N33/68

CPC classification number: G01N33/5767 ,  C07K16/109 ,  G01N33/56966 ,  G01N33/56983 ,  G01N33/686 ,  G01N2333/186 ,  G01N2469/10 ,  G01N2469/20

Abstract: An in vitro method that allows detection of hepatitis C by detecting hepatitis C virus (HCV) core protein and antibodies to HCV core protein (anti-core antibodies) in a single assay is provided. Cross-reactivity is eliminated in the method preferably by utilizing short peptides, each of which has an amino acid sequence that corresponds to an immunodominant region of the native core protein but which does not wholly encompass the epitope bound by the antibodies utilized in the method. The method can be used to detect the presence of HCV in a subject, and/or to determine the suitability of donor blood or blood products for transfusion purposes. Also provided are diagnostic kits for carrying out the method and a process for selecting suitable capture peptides and monoclonal antibodies for use in the combination method.

Abstract translation: 提供了通过在单次测定中检测丙型肝炎病毒（HCV）核心蛋白和HCV核心蛋白（抗核心抗体）抗体来检测丙型肝炎的体外方法。 优选通过利用短肽来消除交叉反应性，所述短肽具有对应于天然核心蛋白的免疫显性区域但不完全包含由该方法中使用的抗体结合的表位的氨基酸序列。 该方法可用于检测受试者中HCV的存在，和/或确定供体血液或血液制品对于输血目的的适用性。 还提供了用于进行该方法的诊断试剂盒和用于选择合适的捕获肽和用于组合方法的单克隆抗体的方法。

公开(公告)号：US20150177242A1

公开(公告)日：2015-06-25

申请号：US14480978

申请日：2014-09-09

Applicant: Abbott Laboratories

Inventor： Brian C. Rodgers ,  Graham J. Burch

IPC: G01N33/569 ,  G01N33/68

CPC classification number: G01N33/5767 ,  C07K16/109 ,  G01N33/56966 ,  G01N33/56983 ,  G01N33/686 ,  G01N2333/186 ,  G01N2469/10 ,  G01N2469/20

Abstract: An in vitro method that allows detection of hepatitis C by detecting hepatitis C virus (HCV) core protein and antibodies to HCV core protein (anti-core antibodies) in a single assay is provided. Cross-reactivity is eliminated in the method preferably by utilizing short peptides, each of which has an amino acid sequence that corresponds to an immunodominant region of the native core protein but which does not wholly encompass the epitope bound by the antibodies utilized in the method. The method can be used to detect the presence of HCV in a subject, and/or to determine the suitability of donor blood or blood products for transfusion purposes. Also provided are diagnostic kits for carrying out the method and a process for selecting suitable capture peptides and monoclonal antibodies for use in the combination method.

Abstract translation: 提供了通过在单次测定中检测丙型肝炎病毒（HCV）核心蛋白和HCV核心蛋白（抗核心抗体）抗体来检测丙型肝炎的体外方法。 优选通过利用短肽来消除交叉反应性，所述短肽具有对应于天然核心蛋白的免疫显性区域但不完全包含由该方法中使用的抗体结合的表位的氨基酸序列。 该方法可用于检测受试者中HCV的存在，和/或确定供体血液或血液制品对于输血目的的适用性。 还提供了用于进行该方法的诊断试剂盒和用于选择合适的捕获肽和用于组合方法的单克隆抗体的方法。