公开(公告)号：US20150065360A1

公开(公告)日：2015-03-05

申请号：US14538379

申请日：2014-11-11

Applicant: Advanced Liquid Logic, Inc.

Inventor： Allen E. Eckhardt ,  Jonathan Benton ,  Deborah Boles

IPC: C12N15/10 ,  C12Q1/70 ,  C12Q1/68

CPC classification number: C12N15/1089 ,  C12Q1/6869 ,  C12Q1/689 ,  C12Q1/701 ,  C12Q1/703 ,  G16B30/00 ,  G16B40/00 ,  C12Q2537/165 ,  C12Q2565/301

Abstract: The systems and methods of the invention provide a guided approach to pyrosequencing (i.e., hybrid pyrosequencing). A de novo nucleic acid sequence may compared to a library of possible results and the next nucleotide to be dispensed is selected based on the comparison of the de novo sequence and the library of possible results. In another example, at least the first nucleotide to be dispensed is selected based on a query of a database(s) of non-sequence parameters (e.g., incidence of infection, diagnostic symptoms, sample source) and subsequent dispensations determined based on a comparison of the de novo sequence and the library of possible results (e.g., candidate sequences). The systems and methods of the invention may be performed using a droplet actuator.

Abstract translation: 本发明的系统和方法提供了焦磷酸测序（即，杂化焦磷酸测序）的指导方法。 从头核酸序列可以与可能结果的文库进行比较，并且基于从头序列与可能结果库的比较来选择待分配的下一个核苷酸。 在另一个实例中，基于对非序列参数（例如，感染发生率，诊断症状，样本来源）的数据库的查询和基于比较确定的随后分配来选择至少要分配的第一核苷酸 的新生序列和可能结果的库（例如候选序列）。 本发明的系统和方法可以使用液滴致动器来执行。