公开(公告)号：US20210106997A1

公开(公告)日：2021-04-15

申请号：US17077828

申请日：2020-10-22

Applicant: Agency for Science, Technology and Research

Inventor： Jackie Y. Ying ,  Min-Han Tan ,  Yoke San Daniel Lee ,  Igor Cima ,  Yeon Joon Park ,  Wai Min Phyo

IPC: B01L3/00 ,  C12N5/071 ,  G01N33/50 ,  G01N33/574 ,  C12Q1/6886

Abstract: A column-based device and method for retrieving cells of interest were enclosed. The said device comprises a column comprising (i) an inner wall defining an inner chamber with inlet and outlet openings, (ii) a perforated plug disposed adjacent to the outlet opening, (iii) a sleeve insert with a channel and disposed within the chamber and adjacent to the perforated plug, and (iv) a filtering means housed within sleeve insert sandwiched between two sealing means. In particular, Tumor-derived endothelial cell clusters (TECCs) as characterized multiple nuclei, expression of endothelial markers (PECAM1, VWF and CDH5), and non-expression of leukocyte, megakaryocyte and platelets markers, may be retrieved using the disclosed device. Also encompassed are methods, reagents and kits for the diagnosis and prognosis of cancers by detecting for the presence of TECCs isolated from blood samples using the claimed device.

公开(公告)号：US20220001385A1

公开(公告)日：2022-01-06

申请号：US17360014

申请日：2021-06-28

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Jackie Y. Ying ,  Min-Han Tan ,  Yoke San Daniel Lee ,  Igor Cima ,  Yeon Joon Park ,  Wai Min Phyo

IPC: B01L3/00 ,  C12N5/071 ,  G01N33/50 ,  G01N33/574 ,  C12Q1/6886

Abstract: A device and method for retrieving cells of interest, in particular rare cells, were disclosed. Tumor-derived endothelial cell clusters (TECCs) retrieved using the disclosed device and method can be used for the diagnosis and prognosis of cancer.

公开(公告)号：US11186865B2

公开(公告)日：2021-11-30

申请号：US15541295

申请日：2016-01-21

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Min-Han Tan ,  Igor Cima

IPC: C12Q1/68 ,  C12Q1/686 ,  C12Q1/6806 ,  C12Q1/6876

Abstract: A method of simultaneously analyzing RNA and DNA in a sample, the method comprising the step (a) contacting the sample with a reverse primer from a first primer pair directed to a target RNA region to effect reverse transcription of RNA into cDNA with a reverse transcriptase; (b) subsequently contacting the sample with (i) a forward primer from the first primer pair directed to a second cDNA region, (ii) a forward and a reverse primers from a second primer pair targeted to a DNA region, and (ii) a DNA polymerase to simultaneously amplify the target cDNA and target DNA region; and (c) analyzing the amplified target cDNA region and/or amplified target DNA region. Also encompassed are uses of the method to analyze gene expression and mutations, kits comprising primers, enzymes, buffers.

公开(公告)号：US20180008982A1

公开(公告)日：2018-01-11

申请号：US15544761

申请日：2016-01-21

Applicant: Agency for Science, Technology and Research

Inventor： Jackie Y. Ying ,  Min-Han Tan ,  Yoke San Daniel Lee ,  Igor Cima ,  Yeon Joon Park ,  Wai Min Phyo

IPC: B01L3/00 ,  C12Q1/68 ,  G01N33/574

CPC classification number: B01L3/502761 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2300/042 ,  B01L2300/0681 ,  C12N5/069 ,  C12Q1/6886 ,  C12Q2600/156 ,  G01N33/5064 ,  G01N33/5094 ,  G01N33/574 ,  G01N33/57419 ,  G01N33/57492

Abstract: A column-based device and method for retrieving cells of interest were enclosed. The said device comprises a column comprising (i) an inner wall defining an inner chamber with inlet and outlet openings, (ii) a perforated plug disposed adjacent to the outlet opening, (iii) a sleeve insert with a channel and disposed within the chamber and adjacent to the perforated plug, and (iv) a filtering means housed within sleeve insert sandwiched between two sealing means. In particular, Tumor-derived endothelial cell clusters (TECCs) as characterized multiple nuclei, expression of endothelial markers (PECAM1, VWF and CDH5), and non-expression of leukocyte, megakaryocyte and platelets markers, may be retrieved using the disclosed device. Also encompassed are methods, reagents and kits for the diagnosis and prognosis of cancers by detecting for the presence of TECCs isolated from blood samples using the claimed device.

公开(公告)号：US11701657B2

公开(公告)日：2023-07-18

申请号：US17077828

申请日：2020-10-22

Applicant: Agency for Science, Technology and Research

Inventor： Jackie Y. Ying ,  Min-Han Tan ,  Yoke San Daniel Lee ,  Igor Cima ,  Yeon Joon Park ,  Wai Min Phyo

IPC: B01L3/00 ,  C12N5/071 ,  G01N33/50 ,  G01N33/574 ,  C12Q1/6886

CPC classification number: B01L3/502761 ,  C12N5/069 ,  C12Q1/6886 ,  G01N33/5064 ,  G01N33/5094 ,  G01N33/574 ,  G01N33/57419 ,  G01N33/57492 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2300/042 ,  B01L2300/0681 ,  C12Q2600/156

Abstract: A column-based device and method for retrieving cells of interest were enclosed. The said device comprises a column comprising (i) an inner wall defining an inner chamber with inlet and outlet openings, (ii) a perforated plug disposed adjacent to the outlet opening, (iii) a sleeve insert with a channel and disposed within the chamber and adjacent to the perforated plug, and (iv) a filtering means housed within sleeve insert sandwiched between two sealing means. In particular, Tumor-derived endothelial cell clusters (TECCs) as characterized multiple nuclei, expression of endothelial markers (PECAM1, VWF and CDH5), and non-expression of leukocyte, megakaryocyte and platelets markers, may be retrieved using the disclosed device. Also encompassed are methods, reagents and kits for the diagnosis and prognosis of cancers by detecting for the presence of TECCs isolated from blood samples using the claimed device.

公开(公告)号：US11084034B2

公开(公告)日：2021-08-10

申请号：US15544761

申请日：2016-01-21

Applicant: Agency for Science, Technology and Research

Inventor： Jackie Y. Ying ,  Min-Han Tan ,  Yoke San Daniel Lee ,  Igor Cima ,  Yeon Joon Park ,  Wai Min Phyo

IPC: C12N5/071 ,  G01N33/50 ,  G01N33/574 ,  B01L3/00 ,  C12Q1/6886

Abstract: A column-based device and method for retrieving cells of interest were enclosed. The said device comprises a column comprising (i) an inner wall defining an inner chamber with inlet and outlet openings, (ii) a perforated plug disposed adjacent to the outlet opening, (iii) a sleeve insert with a channel and disposed within the chamber and adjacent to the perforated plug, and (iv) a filtering means housed within sleeve insert sandwiched between two sealing means. In particular, Tumor-derived endothelial cell clusters (TECCs) as characterized multiple nuclei, expression of endothelial markers (PECAM1, VWF and CDH5), and non-expression of leukocyte, megakaryocyte and platelets markers, may be retrieved using the disclosed device. Also encompassed are methods, reagents and kits for the diagnosis and prognosis of cancers by detecting for the presence of TECCs isolated from blood samples using the claimed device.