公开(公告)号：US20090164130A1

公开(公告)日：2009-06-25

申请号：US11917023

申请日：2006-06-09

Applicant: Anand T. N. Kumar ,  David Alan Boas ,  Adrew K. Dunn

Inventor： Anand T. N. Kumar ,  David Alan Boas ,  Adrew K. Dunn

IPC: G01N21/64 ,  G06F19/00

CPC classification number: G01N21/6456 ,  G01N21/39 ,  G01N21/4795 ,  G01N21/6408 ,  G01N2021/1787 ,  G01N2201/0697 ,  G01N2201/0846 ,  G01N2201/103

Abstract: Methods, apparatus (100), and computer program products for determining lifetimes and distribution of fluorophores (102) embedded in samples (104). Fluorophores are placed into the sample, light from a source (110) selected to excite the fluorophores illuminates the sample, light emitted from the excited fluorophores is detected by a device (138), and a time-domain analysis is performed on the detected emitted light to determine a three-dimensional distribution of the fluorophores in the sample.

Abstract translation: 用于确定嵌入样品（104）中的荧光团（102）的寿命和分布的方法，装置（100）和计算机程序产品。 将荧光团放置在样品中，来自选择用于激发荧光团的源（110）的光照射样品，由装置（138）检测从激发的荧光团发射的光，并且对所检测到的发射 以确定样品中荧光团的三维分布。

公开(公告)号：US09921156B2

公开(公告)日：2018-03-20

申请号：US14765690

申请日：2014-01-31

Applicant: Anand T. N. Kumar

Inventor： Anand T. N. Kumar

IPC: G01N21/64 ,  G01N33/483

CPC classification number: G01N21/6408 ,  G01N33/4833 ,  G01N2021/6439 ,  G01N2201/06113 ,  G01N2201/12

Abstract: A system and method for determining fluorescence decay in a biological sample is provided. The method includes acquiring optical signal data from at least a part of a biological sample undergoing fluorescence, assembling the optical signal data into a set of spatial time-series data, and converting the set of spatial time-series data into a set of spatial frequency time-series data. The method also includes applying a spatial filter to the set of spatial frequency time-series data to yield a set of filtered frequency-series data, the spatial filter configured to separate, from the set of frequency-series data, fluorescence signals consistent with a non-diffuse component and converting the set of filtered frequency-series data into a set of filtered time-series data. The method further includes determining, using the filtered time-series data, a fluorescence signature consistent with fluorescence decay of at least one fluorophore, and generating a report indicative of the fluorescence signature of the biological sample.