公开(公告)号：US20220259580A1

公开(公告)日：2022-08-18

申请号：US17416480

申请日：2019-12-09

Applicant: BASF SE

Inventor： Kai-Uwe Baldenius ,  Michael Breuer ,  Corinna Ruffer ,  Melanie Weingarten ,  Reinhard Zschoche ,  Stefan Seemayer ,  Bernd Nidetzky ,  Katharina Schmoelzer ,  Michael Puhl

IPC: C12N9/24 ,  C12P19/14 ,  C12P19/18 ,  C12N1/20

Abstract: The present disclosure relates to methods for producing human milk oligosaccharide (HMO) core structures using glycosidases from family GH20 hexosaminidases. In particular, the present disclosure provides methods for producing lacto-N-triose II (LNT II) and/or lacto-N-tetraose (LNT) by reacting glucosamine-oxazoline and/or lacto-N-biose-oxazoline with lactose catalysed by an enzyme of the glycoside hydrolase family 20 (GH20) according to the classification of the Carbohydrate-Active-Enzymes (CAZy) database. Specific optimized enzymes are identified to catalyse the reactions.

公开(公告)号：US10533201B2

公开(公告)日：2020-01-14

申请号：US15509993

申请日：2015-06-30

Applicant: BASF SE

Inventor： Vaidotas Navickas ,  Kai-Uwe Baldenius ,  Michael Breuer ,  Patricia Wildberger ,  Martin Pfeiffer ,  Bernd Nidetzky

IPC: C07H11/04 ,  C12P19/02 ,  C12P19/26 ,  C12N9/10 ,  C12N9/16 ,  C07H1/02

Abstract: The present invention relates to methods of producing sugar phosphates by enzymatic transphosphorylation, wherein the phosphoryl transfer from phosphate donor substrates to sugar substrates is catalyzed by an enzyme having alpha-glucose-1-phosphatase activity. Furthermore, the present invention relates to a biocatalyst having sucrose phosphorylase as well as alpha-glucose-1-phosphatase activity and its use. The production of an enzyme having alpha-glucose-1-phosphatase activity and its use in the transphosphorylation of sugar substrates is also presented herein.